Creatinine Biosensor Based on Graphite Paste Electrode Modified with Dibenzo-30-crown-10 and Rice Husk

Abstract

A novel electrochemical creatinine biosensor based on graphite paste electrode modified with dibenzo-30-crown-10 (DB30C10) and rice husk (RH) has been developed for selective and quantitative recognition of creatinine by immobilizing enzyme creatinase for monitoring the amperometric response. Creatinase immobilization on electrode was investigated using amperometric method and factors affecting its immobilization such as potential as well as pH was discussed. The performance of the developed creatinine biosensor was evaluated and the obtained creatinine biosensor exhibited shorter response time (3 s), wider range 0.1-30 µM and the detection limit were found to be 1 × 10 -7 M. About 90 % of the enzyme activity is retained for about 60 days.