Creatinine Deiminase Adsorption onto Silicalite-Modified pH-FET for Creation of New Creatinine-Sensitive Biosensor

Abstract

In the work, silicalite particles were used for the surface modification of pH-sensitive field-effect transistors (pH-FETs) with the purpose of developing new creatinine-sensitive biosensor. Creatinine deiminase (CD) adsorbed on the surface of silicalite-coated pH-FET served as a bioselective membrane. The biosensor based on CD immobilized in glutaraldehyde vapor (GA) was taken as control. The creatinine-sensitive biosensor obtained by adsorption on silicalite was shown to have better analytical characteristics (two- to threefold increased sensitivity to creatinine, three- to fourfold lesser response and recovery times, a decrease of the detection limit of creatinine determination to 5 μM, etc.).Additionally, the biosensor based on CD adsorbed on silicalite (Sil/CD) was characterized by high signal reproducibility (relative standard deviation (RSD) for creatinine measurement = 2.6 %) and stability during storage (over 13 months). It was experimentally confirmed that the proposed biosensor was not sensitive either to high concentrations of sodium chloride or to the macromolecular protein fractions and can be used for direct quantitative analysis of creatinine in the blood serum.It was concluded that the method of CD adsorption on silicalite is well-suited for the creation of creatinine-sensitive biosensor with improved working characteristics.

Electrochemical Creatinine (Bio)Sensors for Point-of-Care Diagnosis of Renal Malfunction and Chronic Kidney Disorders

“…Furthermore, to reduce such interference, ion-selective field-effect transistors (ISFET) are constructed with a thin layer of enzymes immobilized over the ion-selective membrane, thereby making them enzyme-sensitive FETs. ISFETs can be based on NH 4 + -selective FETs or pH-sensitive FETs [79,80]. Operational/storage stability, sensitivity, and potential inhibition to interference are determined by the design of the electrode surface and enzyme immobilization technique.…”

Overexpression and one‐step renaturation‐purification of the tagged creatinine deiminase of Corynebacterium glutamicum in Escherichia coli cells

“…Commercially available CDI has been used to develop different approaches for creatinine assay. However, the cost of the enzyme is rather high, possibly, due to a low protein production by the recombinant strain and a complex purification procedure (Gottschalk et al, 2007; Marchenko et al, 2016; Zakalskiy et al, 2019).…”

Biosensors Based on Nano-Gold/Zeolite-Modified Ion Selective Field-Effect Transistors for Creatinine Detection

“…We have previously shown the development of new methods for potentiometric [ 20 , 21 ], amperometric [ 22 ] and conductometric [ 8 , 23 ] transducers based on immobilization of enzymes on zeolites. In our recent study, owing to the unique advantages such as easiness, fastness, inexpensiveness and ability to analyse the biological content as electrical signal of electrochemical biosensors with an additional advantage of miniaturized silicon-based semiconductor nature of field-effect transistor (FET)-based sensors, we have developed a novel approach for constructing an ion selective field-effect transistor (ISFET) device for creatinine monitoring using silicalite [ 24 ]. The objective of this study was to combine the advantages of using zeolites with different properties (i.e.…”