Creation of a neovagina in patients with Rokitansky syndrome using peritoneum from the pouch of Douglas: an analysis of 48 cases

Marques, Herbert de Sousa; Santos, Francisca Lopes dos; Lopes-Costa, Pedro Vitor; Santos, Adaniel Sousa dos; Silva, Benedito Borges da

doi:10.1016/j.fertnstert.2007.07.1321

Cited by 26 publications

(3 citation statements)

References 19 publications

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“…Various studies have evaluated the functional and anatomic evaluation of several operative (7,8,(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30) and nonoperative (31, 32) vaginoplasty methods in patients with vaginal agenesis due to MRKH syndrome. However, no ''ideal''…”

Section: Discussionmentioning

confidence: 99%

“…Various authors have reported on the use of several artificial or biologic materials to cover the neovaginal cavity and induce epithelialization. Autologous human amniotic membranes (14,16,21,30), homologous amniotic membranes (20), peritoneal layers of the pouch of Douglas (13), artificial dermis and recombinant basic fibroblast growth factor (7), split-thickness skin graft from the buttocks (29), oxidized cellulose (18), acellular human dermal allograft (26) and autologous in vitro-cultured vaginal tissue (27) are some of the applied tissues that have been named.…”

Section: Mean ± Sd (Range)mentioning

confidence: 99%

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Functional and anatomic results of amnion vaginoplasty in young women with Mayer-Rokitansky-Küster-Hauser syndrome

Fotopoulou

Sehouli

Gehrmann

et al. 2010

Fertility and Sterility

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Section: Discussionmentioning

confidence: 99%

Section: Mean ± Sd (Range)mentioning

confidence: 99%

Functional and anatomic results of amnion vaginoplasty in young women with Mayer-Rokitansky-Küster-Hauser syndrome

Fotopoulou

Sehouli

Gehrmann

et al. 2010

Fertility and Sterility

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“…The procedure consists in the creation of a vaginal tunnel that is subsequently covered by a full-thickness skin graft [3]. In the modified McIndoe technique other materials are used to line the neovaginal cavity, such as autologous human amniotic membranes [4], peritoneal layers of the pouch of Douglas [5], and intestinal grafts [6]. In 2007, we presented the first case of autologous in vitro cultured vaginal tissue transplantation for the epithelialization of the neovagina walls [7].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Human Vaginal Mucosa Cells for Autologous In Vitro Cultured Vaginal Tissue Transplantation in Patients with MRKH Syndrome

Nodale

Vescarelli

D'Amici

et al. 2014

BioMed Research International

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Mayer-Rokitansky-Küster-Hauser (MRKH) is a rare syndrome characterized by congenital aplasia of the uterus and vagina. The most common procedure used for surgical reconstruction of the neovagina is the McIndoe vaginoplasty, which consists in creation of a vaginal canal covered with a full-thickness skin graft. Here we characterized the autologous in vitro cultured vaginal tissue proposed as alternative material in our developed modified McIndoe vaginoplasty in order to underlie its importance in autologous total vaginal replacement. To this aim human vaginal mucosa cells (HVMs) were isolated from vaginal mucosa of patients affected by MRKH syndrome and characterized with respect to growth kinetics, morphology, PAS staining, and expression of specific epithelial markers by immunofluorescence, Western blot, and qRT-PCR analyses. The presence of specific epithelial markers along with the morphology and the presence of mucified cells demonstrated the epithelial nature of HMVs, important for an efficient epithelialization of the neovagina walls and for creating a functional vaginal cavity. Moreover, these cells presented characteristics of effective proliferation as demonstrated by growth kinetics assay. Therefore, the autologous in vitro cultured vaginal tissue might represent a highly promising and valid material for McIndoe vaginoplasty.

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Vulvo-perineoplasty/Creatsas Vaginoplasty

Creatsas

Creatsa

2014

Female Genital Tract Congenital Malformations

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Creation of a neovagina in patients with Rokitansky syndrome using peritoneum from the pouch of Douglas: an analysis of 48 cases

Cited by 26 publications

References 19 publications

Functional and anatomic results of amnion vaginoplasty in young women with Mayer-Rokitansky-Küster-Hauser syndrome

Functional and anatomic results of amnion vaginoplasty in young women with Mayer-Rokitansky-Küster-Hauser syndrome

Characterization of Human Vaginal Mucosa Cells for Autologous In Vitro Cultured Vaginal Tissue Transplantation in Patients with MRKH Syndrome

Vulvo-perineoplasty/Creatsas Vaginoplasty

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