Creation of an Acellular Vaginal Matrix for Potential Vaginal Augmentation and Cloacal Repair

Greco, Karin Vicente; Jones, Lauren G.; Obiri-Yeboa, Irene; Ansari, Tahera

doi:10.1016/j.jpag.2018.05.003

Cited by 25 publications

(21 citation statements)

References 21 publications

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“…30 In another study combining Triton X-100 with SD and DNase/RNase, Greco et al created an acellular porcine vaginal matrix that could be used for vaginal augmentation and cloacal repair. 31 Importantly, the authors reported retention of the ECM’s collagen and elastin, 50% of GAGs and an intact base membrane (positive for laminin and collagen IV). 31 The decellularised scaffolds were seeded with adipose-derived stem cells (ADSCs) and vaginal epithelial cells showing attachment and growth.…”

Section: Methods To Produce Decellularised Matricesmentioning

confidence: 97%

“…31 Importantly, the authors reported retention of the ECM’s collagen and elastin, 50% of GAGs and an intact base membrane (positive for laminin and collagen IV). 31 The decellularised scaffolds were seeded with adipose-derived stem cells (ADSCs) and vaginal epithelial cells showing attachment and growth. 31 This study highlights the importance of combining different chemical agents to obtain efficient decellularisation.…”

Section: Methods To Produce Decellularised Matricesmentioning

confidence: 97%

“…31 The decellularised scaffolds were seeded with adipose-derived stem cells (ADSCs) and vaginal epithelial cells showing attachment and growth. 31 This study highlights the importance of combining different chemical agents to obtain efficient decellularisation.…”

Section: Chemical Methodsmentioning

confidence: 99%

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Decellularised scaffolds: just a framework? Current knowledge and future directions

et al. 2020

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The use of decellularised matrices as scaffolds offers the advantage of great similarity with the tissue to be replaced. Moreover, decellularised tissues and organs can be repopulated with the patient’s own cells to produce bespoke therapies. Great progress has been made in research and development of decellularised scaffolds, and more recently, these materials are being used in exciting new areas like hydrogels and bioinks. However, much effort is still needed towards preserving the original extracellular matrix composition, especially its minor components, assessing its functionality and scaling up for large tissues and organs. Emphasis should also be placed on developing new decellularisation methods and establishing minimal criteria for assessing the success of the decellularisation process. The aim of this review is to critically review the existing literature on decellularised scaffolds, especially on the preparation of these matrices, and point out areas for improvement, finishing with alternative uses of decellularised scaffolds other than tissue and organ reconstruction. Such uses include three-dimensional ex vivo platforms for idiopathic diseases and cancer modelling.

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Section: Methods To Produce Decellularised Matricesmentioning

confidence: 97%

Section: Methods To Produce Decellularised Matricesmentioning

confidence: 97%

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Decellularised scaffolds: just a framework? Current knowledge and future directions

et al. 2020

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“…ECM products that are obtained from decellularization of female reproductive organs have salient roles in the field of reproductive medicine, with research focusing mainly on those that are derived from the uterus [ 51 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 ], ovary [ 50 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 ], cervix [ 93 ], vagina [ 94 , 95 ], and placenta [ 96 , 97 , 98 , 99 , 100 , 101 , 102 , 103 , 104 , 105 ]. The variable methods that are used for decellularization are dependent on the tissue type and its characteristics.…”

Section: Deriving Ecm Hydrogels From Reproductive Organsmentioning

confidence: 99%

Future Challenges and Opportunities of Extracellular Matrix Hydrogels in Female Reproductive Medicine

Francés-Herrero

Rodríguez-Eguren

Gómez-Álvarez

et al. 2022

IJMS

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Bioengineering and reproductive medicine have progressed shoulder to shoulder for several decades. A key point of overlap is the development and clinical translation of technologies to support reproductive health, e.g., scaffold-free constructs, polymeric scaffolds, bioprinting or microfluidics, and hydrogels. Hydrogels are the focus of intense study, and those that are derived from the extracellular matrix (ECM) of reproductive tissues and organs are emerging as promising new players given their results in pre-clinical models. This literature review addresses the recent advances in the use of organ-specific ECM hydrogels in reproductive medicine, considering the entire female reproductive tract. We discuss in-depth papers describing the development of ECM hydrogels, their use in in vitro models, and their in vivo application in preclinical studies. We also summarize the functions of hydrogels, including as grafts, carriers for cell transplantation, or drug depots, and present the potential and possible scope for use of ECM hydrogels in the near future based on recent scientific advances.

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“…In the future we hope that tissue engineered vagina (20) becomes available at this point in the operation and confines vaginal replacement with bowel to the history books.…”

Section: Factors In the Initial Assessment Which Affect Treatmentmentioning

confidence: 99%

Cloacal Malformations: Technical Aspects of the Reconstruction and Factors Which Predict Surgical Complexity

2019

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Cloacal malformations are rare anomalies which occur in one in 50,000 live births. Anatomically these anomalies are defined by the presence of a single perineal orifice. There is however a substantial range in their complexity. Defining these differences is key to surgical planning and timely referral of selected cases to centers with the capabilities to manage the most challenging cases. Traditionally the common channel length as measured during cysto-vaginoscopy has been used to differentiate between patients that can be repaired with a reproducible operation and those requiring a more complex repair. The quality and range of imaging available has advanced and thus a more detailed anatomic picture is now possible to help with pre-operative planning. Cross sectional imaging with 3D reconstruction has enhanced the understanding of the anatomic variations in these patients. In addition, the sacral ratio, previously thought to only have an influence on long term continence predictions, has been shown to not only forecast the presence of urological anomalies, but also the complexity of the malformation. In principle, cloacal malformations have two major components to the reconstruction. First, the rectum needs to be separated from the urogenital tract and second, the urogenital sinus needs to be managed to create a urethral orifice and vaginal introitus. The length of the urethra has been shown to be vital in deciding between the two main surgical maneuvers; a total urogenital mobilization (TUM) and a urogenital separation. The technical demands of a urogenital separation are significant and discussed here in detail. The need for vaginal replacement adds further complexity to the care of these patients and has also been shown to be related to the length of the urethra. Predicting complexity in an accurate and non-invasive way will facilitate the care of the most complex cloacal malformations and improve outcomes.

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Creation of an Acellular Vaginal Matrix for Potential Vaginal Augmentation and Cloacal Repair

Cited by 25 publications

References 21 publications

Decellularised scaffolds: just a framework? Current knowledge and future directions

Decellularised scaffolds: just a framework? Current knowledge and future directions

Future Challenges and Opportunities of Extracellular Matrix Hydrogels in Female Reproductive Medicine

Cloacal Malformations: Technical Aspects of the Reconstruction and Factors Which Predict Surgical Complexity

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