CREB-binding Protein/p300 Co-activation of Crystallin Gene Expression

Chen, Qin; Dowhan, Dennis H.; Liang, Diana H.; Moore, David D.; Overbeek, Paul A.

doi:10.1074/jbc.m201821200

Cited by 80 publications

(72 citation statements)

References 34 publications

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“…Maf family members also frequently cooperate with members of other transcription factor families (Ho et al, 1996,Huang et al, 2002,Rajaram and Kerppola, 2004,Sevinsky et al, 2004,Aramata et al, 2005 and can also interact with transcriptional coactivators, such as CREB-binding protein and p300 to activate gene transcription (Chen et al, 2002). In our hands co-transfection of c-Maf with either Ets-2 or c-Jun expression plasmids did not show synergistic effects between these transcription factors on the CD13 proximal promoter (data not shown).…”

Section: Discussionmentioning

confidence: 52%

CD13/APN transcription is regulated by the proto-oncogene c-Maf via an atypical response element

Mahoney

Petrović

Schacke

et al. 2007

Gene

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Angiogenic growth factors induce the transcription of the cell surface peptidase CD13/APN in activated endothelial cells of the tumor vasculature. Inhibition of CD13/APN abrogates endothelial invasion and morphogenesis in vitro and tumor growth in vivo suggesting a critical functional role for CD13 in angiogenesis. Experiments to identify the transcription factors responsible for this regulation demonstrated that exogenous expression of the proto-oncogene c-Maf, but not other bZip family members tested, potently activates transcription from a critical regulatory region of the CD13 proximal promoter between −115 and −70 bp which is highly conserved among mammalian species. Using promoter mutation, EMSA and ChIP analyses we established that both endogenous and recombinant c-Maf directly interact with an atypical Maf response element contained within this active promoter region via its basic DNA/leucine zipper domain. However full activity of c-Maf requires the amino-terminal transactivation domain, and site-directed mutation of putative phosphorylation sites within the transactivation domain (serines 15 and 70) shows that these sites behave in a dramatic cell type-specific manner. Therefore, this atypical response element predicts a broader range of c-Maf target genes than previously appreciated and thus impacts its regulation of multiple myeloma as well as endothelial cell function and angiogenesis.

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Section: Discussionmentioning

confidence: 52%

CD13/APN transcription is regulated by the proto-oncogene c-Maf via an atypical response element

Mahoney

Petrović

Schacke

et al. 2007

Gene

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“…Recently, it has become apparent that Prox1 probably interacts with other proteins while performing its functions. Prox1 can bind to CBP/p300, a transcriptional cofactor, and work synergistically with Maf to activate the mouse ␤B2-crystallin promoter (48). It is possible that Maf and Prox1, respectively, bind to adjacent cis elements, PL2 and OL2, and cooperatively recruit CBP/p300 to the chicken ␤B1-crystallin promoter as they do for the mouse ␤B2-crystallin promoter.…”

Section: Discussionmentioning

confidence: 99%

“…Prox1 and Mafs Cooperate to Activate the Chicken ␤B1-Crystallin Promoter-It has been shown that Prox1 and the c-Maf short form synergistically activate the mouse ␤B2-crystallin promoter in transfection assays (48). Here we investigate whether Prox1 and Mafs can cooperatively activate the ␤B1-crystallin promoter in transfection assays.…”

Section: Figmentioning

confidence: 99%

Mafs, Prox1, and Pax6 Can Regulate Chicken βB1-Crystallin Gene Expression

Cui

Tomarev

Piatigorsky

et al. 2004

Journal of Biological Chemistry

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During lens fiber cell differentiation, the regulation of crystallin gene expression is coupled with dramatic morphological changes. Here we report that Mafs, Prox1, and Pax6, which are essential transcription factors for normal lens development, bind to three functionally important cis elements, PL1, PL2, and OL2, in the chicken ␤B1-crystallin promoter and may cooperatively direct the transcription of this lens fiber cell preferred gene. Gel shift assays demonstrated that Mafs bind to the MARE-like sequences in the PL1 and PL2 elements, whereas Prox1, a sequence-specific DNA-binding protein like its Drosophila homolog Prospero, interacts with the OL2 element. Furthermore, Pax6, a known repressor of the chicken ␤B1-crystallin promoter, binds to all three of these cis elements. In transfection assays, Mafs and Prox1 activated the chicken ␤B1-crystallin promoter; however, their transactivation ability was repressed when co-transfected with Pax6. Taken together with the known spatiotemporal expression patterns of Mafs, Prox1, and Pax6 in the developing lens, we propose that Pax6 occupies and represses the chicken ␤B1-crystallin promoter in lens epithelial cells, and is displaced by Prox1 and Mafs, which activate the promoter, in differentiating cortical fiber cells.

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“…Both p300 and CBP interact with lens-regulatory factors such as CREB (Chrivia et al, 1993), Pax6 (Hussain and Habener, 1999) and c-Maf (Q. Chen et al, 2002). Mutations in human CBP and less frequently in its homologue gene p300, cause Rubinstein-Taybi syndrome, which manifests with a variety of ocular defects including cataracts (van Genderen et al, 2000).…”

Section: Histone Modifications and Histone-modifying Enzymes-postransmentioning

confidence: 99%

Genetic and epigenetic mechanisms of gene regulation during lens development

Cvekl

Duncan

2007

Progress in Retinal and Eye Research

141

124

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Recent studies demonstrated a number of links between chromatin structure, gene expression, extracellular signaling and cellular differentiation during lens development. Lens progenitor cells originate from a pool of common progenitor cells, the pre-placodal region (PPR) which is formed due to a complex exchange of extracellular signals between the neural plate, naïve ectoderm and mesendoderm. A specific commitment to the lens program over alternate choices such as the formation of olfactory epithelium or the anterior pituitary is manifested by the formation of a thickened surface ectoderm, the lens placode. Mouse lens progenitor cells are characterized by the expression of a complement of lens lineage-specific transcription factors including Pax6, Six3 and Sox2, controlled by FGF and BMP signaling, followed later by c-Maf, Mab21like1, Prox1 and FoxE3. Proliferation of lens progenitors together with their morphogenetic movements results in the formation of the lens vesicle. This transient structure, comprised of lens precursor cells, is polarized with its anterior cells retaining their epithelial morphology and proliferative capacity, whereas the posterior lens precursor cells initiate terminal differentiation forming the primary lens fibers. Lens differentiation is marked by expression and accumulation of crystallins and other structural proteins. The transcriptional control of crystallin genes is characterized by the reiterative use of transcription factors required for the establishment of lens precursors in combination with more ubiquitously expressed factors (e.g. AP-1, AP-2α, CREB and USF) and recruitment of histone acetyltransferases (HATs) CBP and p300, and chromatin remodeling complexes SWI/SNF and ISWI. These studies have poised the study of lens development at the forefront of efforts to understand the connections between development, cell signaling, gene transcription and chromatin remodeling.

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CREB-binding Protein/p300 Co-activation of Crystallin Gene Expression

Cited by 80 publications

References 34 publications

CD13/APN transcription is regulated by the proto-oncogene c-Maf via an atypical response element

CD13/APN transcription is regulated by the proto-oncogene c-Maf via an atypical response element

Mafs, Prox1, and Pax6 Can Regulate Chicken βB1-Crystallin Gene Expression

Genetic and epigenetic mechanisms of gene regulation during lens development

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