CREB-binding proteins (CBP) as a transcriptional coactivator of GATA-2

Jiang, Huijie; Liu, Linde; Yang, Shiming; Tomomi, Takahashi; Nakano, Tsutomu

doi:10.1007/s11427-008-0038-4

Cited by 6 publications

(9 citation statements)

References 32 publications

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“…These transcription factors physically interact with Creb1 and its cofactor Crebbp, respectively (Hong et al. 2006; Jiang et al. 2008), which were also 1.9‐fold up–regulated in expression.…”

Section: Resultsmentioning

confidence: 99%

The exceptional properties of 9‐methyl‐β‐carboline: stimulation, protection and regeneration of dopaminergic neurons coupled with anti‐inflammatory effects

Polanski

Enzensperger

Reichmann

et al. 2010

Journal of Neurochemistry

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J. Neurochem. (2010) 113, 1659–1675. Abstract β‐Carbolines (BCs) are potential endogenous and exogenous neurotoxins that may contribute to the pathogenesis of Parkinson’s disease. However, we recently demonstrated protective and stimulatory effects of 9‐methyl‐BC (9‐me‐BC) in primary dopaminergic culture. In the present study, treatment with 9‐me‐BC unmasked a unique tetrad of effects. First, tyrosine hydroxylase (TH) expression was stimulated in pre‐existing dopa decarboxylase immunoreactive neurons and several TH‐relevant transcription factors (Gata2, Gata3, Creb1, Crebbp) were up‐regulated. Neurite outgrowth of TH immunoreactive (THir) neurons was likewise stimulated. The interaction with tyrosine kinases (protein kinase A and C, epidermal growth factor‐receptor, fibroblast growth factor‐receptor and neural cell adhesion molecule) turned out to be decisive for these observed effects. Second, 9‐me‐BC protected in acute toxicity models THir neurons against lipopolysaccharide and 2,9‐dime‐BC+ toxicity. Third, in a chronic toxicity model when cells were treated with 9‐me‐BC after chronic rotenone administration, a pronounced regeneration of THir neurons was observed. Fourth, 9‐me‐BC inhibited the proliferation of microglia induced by toxin treatment and installed an anti‐inflammatory environment by decreasing the expression of inflammatory cytokines and receptors. Finally, 9‐me‐BC lowered the content of α‐synuclein protein in the cultures. The presented results warrant the exploration of 9‐me‐BC as a novel potential anti‐parkinsonian medication, as 9‐me‐BC interferes with several known pathogenic factors in Parkinson’s disease as outlined above. Further investigations are currently under way.

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Section: Resultsmentioning

confidence: 99%

The exceptional properties of 9‐methyl‐β‐carboline: stimulation, protection and regeneration of dopaminergic neurons coupled with anti‐inflammatory effects

Polanski

Enzensperger

Reichmann

et al. 2010

Journal of Neurochemistry

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“…As a result, recent attempt have been made to therapeutically target CBP/p300 for prostate cancer (36). Moreover, p300/CBP function is also pivotal for transcriptional function of the pioneer factor GATA2 (10, 37). Concordantly, curcumin resulted in reduced occupancy of GATA2 to regulatory loci.…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, GATA2 and FOXA1 function as pioneer factors for multiple nuclear receptors and are associated with poor prognosis (1, 43–45). In tumors wherein CBP function is known to be necessary for GATA2 activity (37), curcumin may prove promising therapeutic agent. Conversely, in tumors wherein GATA2 function is repressed by HDAC3 (46), curcumin mediated-CBP repression may increase response to HDAC3-targeted therapies.…”

Section: Discussionmentioning

confidence: 99%

Targeting Pioneering Factor and Hormone Receptor Cooperative Pathways to Suppress Tumor Progression

2012

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Nuclear receptors and pioneer factors drive the development and progression of prostate cancer. In this disease, aggressive disease phenotypes and hormone therapy failures result from resurgent activity of androgen receptor (AR) and the upregulation of coactivator protein p300 and pioneer factors (e.g. GATA2 and FOXA1). Thus, a major current emphasis in the field is to identify mechanisms by which castrate-resistant AR activity and pioneer factor function can be combinatorially suppressed. Here we show that the turmeric spice isoflavone curcumin suppresses p300 and CBP occupancy at sites of AR function. Curcumin reduced the association of histone acetylation and pioneer factors, thereby suppressing AR residence and downstream target gene expression. Histone deacetylase inhibitors reversed the effects of curcumin on AR activity, further underscoring the impact of curcumin on altering the chromatin landscape. These functions precluded pioneer factor occupancy, leading ultimately to a suppression of ligand-dependent and ligand-independent AR residence on chromatin. Moreover, these functions were conserved even in cells with heightened pioneer factor activity, thus identifying a potential strategy to manage this subclass of tumors. Biological relevance was further identified using in vivo xenograft models mimicking disease progression. Curcumin cooperated in vivo with androgen deprivation as indicated by illustrated by a reduction in tumor growth and delay to the onset of castrate-resistant disease. Together, our results demonstrate the combinatorial impact of targeting AR and histone modification in prostate cancer, setting the stage for further development of curcumin as a novel agent to target AR signaling.

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“…GATA may also squelch promoter activity by competing for common co-activators with other transcription factors known to be critical for LHb gene expression in the gonadotrope. For example, CREB-binding protein is known to be an important co-activator for GATA as well as both SF-1 and LRH-1 (Monte et al 1998, Robert et al 2006, Jiang et al 2008. As yet another possibility, GATA may be recruiting inhibitory FOG2 present in LbT2 cells to the promoter as proposed by Tremblay on the MIS promoter (Tremblay et al 2001).…”

Section: Discussionmentioning

confidence: 98%

GATA transcription factors regulate LHβ gene expression

Lo¹,

Zheng²,

Gong³

et al. 2011

Journal of Molecular Endocrinology

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The GATA family of transcription factors are critical determinants of cell differentiation as well as regulation of adult gene expression throughout the reproductive axis. Within the anterior pituitary gland, GATA factors have been shown to increase glycoprotein a-subunit gene promoter activity; however, nothing has been known about the impact of these factors on expression of the gonadotropin b-subunits. In this study, we demonstrate expression of both GATA2 and GATA4 in primary mouse gonadotropes and the gonadotrope cell line, LbT2. Based on the transient transfection in fibroblast cells, GATA factors increase LH b-subunit gene (LHb) promoter activity alone and in synergy with the orphan nuclear receptors steroidogenic factor-1 (SF-1) and liver receptor homologue-1 (LRH-1). The GATA response was localized to a DNA regulatory region at position K101 in the rat LHb gene promoter which overlaps with a previously described cis-element for pituitary homeobox-1 (Pitx1) and is flanked by two SF-1/LRH-1 regulatory sites. As determined by gel shift, GATA and Pitx1 can compete for binding to this element. Furthermore, mutation analysis revealed a requirement for both the GATA/Pitx1 and the SF-1/LRH-1 cis-elements in order to achieve synergy. These studies identify a novel role for GATA transcription factors in the pituitary and reveal additional molecular mechanisms by which precise modulation of LHb gene expression can be achieved.

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CREB-binding proteins (CBP) as a transcriptional coactivator of GATA-2

Cited by 6 publications

References 32 publications

The exceptional properties of 9‐methyl‐β‐carboline: stimulation, protection and regeneration of dopaminergic neurons coupled with anti‐inflammatory effects

The exceptional properties of 9‐methyl‐β‐carboline: stimulation, protection and regeneration of dopaminergic neurons coupled with anti‐inflammatory effects

Targeting Pioneering Factor and Hormone Receptor Cooperative Pathways to Suppress Tumor Progression

GATA transcription factors regulate LHβ gene expression

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