GATA transcription factors regulate LHβ gene expression

Lo, Ann; Zheng, Weiming; Gong, Yimei; Crochet, John R.; Halvorson, Lisa M.

doi:10.1530/jme-10-0137

Cited by 31 publications

(19 citation statements)

References 47 publications

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“…Further, bmp7 is involved in androgen-dependent development of secondary sexual characteristics in medaka [66]. GATA binding protein 2 ( gata2 , scaffold 9) is a zinc finger transcription factor that is expressed in a sexually dimorphic fashion during mouse gonadogenesis [67], and is known to regulate the expression of gonadotropins including lhb [68, 69], found in the LG13 ZW interval. Cyp27b1 (scaffold 66) encodes 25-Hydroxyvitamin D3 1-alpha-hydroxylase , the rate-limiting enzyme in synthesis of the active form of Vitamin D3; female Cyp27b1 knock out mice have extensive reproductive defects, including delayed puberty, uterine hypoplasia, altered estrogen and gonadotropin levels, and infertility [70–72].…”

Section: Resultsmentioning

confidence: 99%

Polygenic sex determination in the cichlid fish Astatotilapia burtoni

et al. 2016

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BackgroundThe East African riverine cichlid species Astatotilapia burtoni serves as an important laboratory model for sexually dimorphic physiology and behavior, and also serves as an outgroup species for the explosive adaptive radiations of cichlid species in Lake Malawi and Lake Victoria. An astounding diversity of genetic sex determination systems have been revealed within the adaptive radiation of East African cichlids thus far, including polygenic sex determination systems involving the epistatic interaction of multiple, independently segregating sex determination alleles. However, sex determination has remained unmapped in A. burtoni. Here we present mapping results supporting the presence of multiple, novel sex determination alleles, and thus the presence of polygenic sex determination in A. burtoni.ResultsUsing mapping in small families in conjunction with restriction-site associated DNA sequencing strategies, we identify associations with sex at loci on linkage group 13 and linkage group 5–14. Inheritance patterns support an XY sex determination system on linkage group 5–14 (a chromosome fusion relative to other cichlids studied), and an XYW system on linkage group 13, and these associations are replicated in multiple families. Additionally, combining our genetic data with comparative genomic analysis identifies another fusion that is unassociated with sex, with linkage group 8–24 and linkage group 16–21 fused in A. burtoni relative to other East African cichlid species.ConclusionsWe identify genetic signals supporting the presence of three previously unidentified sex determination alleles at two loci in the species A. burtoni, strongly supporting the presence of polygenic sex determination system in the species. These results provide a foundation for future mapping of multiple sex determination genes and their interactions. A better understanding of sex determination in A. burtoni provides important context for their use in behavioral studies, as well as studies of the evolution of genetic sex determination and sexual conflicts in East African cichlids.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3177-1) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Polygenic sex determination in the cichlid fish Astatotilapia burtoni

et al. 2016

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“…The ability of SF1 to act cooperatively with other transcription factors is well documented. SF1 synergizes with several transcription factors, including GATA transcription factors (73)(74)(75)(76), cAMP regulatory element binding proteins (77)(78)(79), AP1 family members ( 80 ), and ␤ -catenin ( 81 ). Signifi cantly, the likelihood of a physical interaction between SF1 and SREBP1 is supported by studies demonstrating that both SREBP1 and SREBP2 interact with hepatic nuclear factor 4 ( 82 ) and with the liver receptor homolog (LRH)1 ( 83 ).…”

Section: Discussionmentioning

confidence: 99%

cAMP-stimulated transcription of DGKθ requires steroidogenic factor 1 and sterol regulatory element binding protein 1

Cai

Sewer

2013

Journal of Lipid Research

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( 4 ). DGK -null mice exhibit several neural abnormalities, including a higher resistance of electroconvulsive shock ( 5 ) and increased cyclooxygenase 2 and tyrosine hydroxylase expression ( 6 ), suggesting a role for DGK in regulating synaptic activity. Mice lacking DGK ␣ ( 7 ) or DGK ( 8 ) exhibit enhanced T cell function and demonstrate a role for these kinases in controlling DAG metabolism during the immune response. DGK isoforms have been implicated in various other cellular processes including inhibition of Rap1 signaling ( 9 ) and retinoblastoma-mediated cell cycle control ( 10 ). DGK is activated by nerve growth factor in PC12 cells ( 11 ) and thrombin in IIC9 fi broblasts ( 12, 13 ), whereas DGK promotes myogenesis in C2C12 cells ( 14 ) and DGK ␥ plays a role in regulating the cell cycle in CHO-K cells ( 15 ).To date, 10 mammalian DGKs have been identifi ed that are divided into fi ve groups based on functional domains ( 16,17 ). However, all isoforms contain cysteine-rich zinc fi nger-like structures, a conserved catalytic region ( 18-21 ). DGK , the sole member of group V, is comprised of three cysteine-rich domains (CRDs), a proline/glycine-rich domain at its N terminus, and a pleckstrin homology (PH) with an overlapping Ras-binding domain ( 22 ). While the functions of many of the other domains in DGK are unclear, the catalytic activity requires all domains of the enzyme ( 23 ). It has been postulated that the CRDs of the enzyme are required both for correct folding of the protein and for substrate presentation ( 23 ). Mutation of the CRD of DGK diminishes DAG-induced translocation of the enzyme to the plasma membrane ( 24 ); whereas the interaction between DGK and the nuclear receptor steroidogenic factor 1 (SF1) requires the PH domain ( 25 ). Abstract Diacylglycerol kinase (DGK) is

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“…The average decrease in Gata4 mRNA levels using siRNAs directed against Gata4 was 67 +/−2% and 70 +/− 2% in absence and presence of GnRH, respectively (data not shown). We have previously demonstrated a 60-70% decrease in GATA protein expression using an identical protocol (Lo et al 2011). …”

Section: Resultsmentioning

confidence: 97%

“…5B) significantly increased the stimulatory effect of GnRH on Adcyap1 promoter activity. Note that the GATA response is lower in the gonadotrope cell line as these cells express high levels of endogenous GATA protein (Lo et al 2011). …”

Section: Resultsmentioning

confidence: 99%

“…During development, GATA2 directs cells towards the gonadotrope lineage and away from the thyrotrope lineage through reciprocal interactions with Pit1 (Dasen, et al 1999). Functional GATA cis-elements have also been identified in the promoter regions of the common α - and Fshb -subunit genes, as well as the Gnrh gene (Lo, et al 2011; Pincas, et al 2001a; Steger, et al 1994). Our laboratory has recently demonstrated that the adult mouse pituitary expresses GATA4 in addition to GATA2 and that both of these factors stimulate gonadotropin Lhb gene expression in the LβT2 gonadotrope cell line (Lo et al 2011).…”

Section: Introductionmentioning

confidence: 99%

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GATA augments GNRH-mediated increases in Adcyap1 gene expression in pituitary gonadotrope cells

Thomas¹,

Crawford²,

Grafer³

et al. 2013

Journal of Molecular Endocrinology

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Pituitary adenylate cyclase-activating polypeptide 1 (PACAP or ADCYAP1) regulates gonadotropin biosynthesis and secretion, both alone and in conjunction with gonadotropin-releasing hormone (GnRH). Initially identified as a hypothalamic-releasing factor, ADCYAP1 subsequently has been identified in pituitary gonadotropes, suggesting it may act as an autocrine-paracrine factor in this tissue. GnRH has been shown to increase pituitary Adcyap1 gene expression though the interaction of CREB and jun/fos with CRE/AP-1 cis-elements in the proximal promoter. For the current studies, we were interested in identifying additional transcription factors and cognate cis-elements which regulate Adcyap1 gene promoter activity and chose to focus on the GATA family of transcription factors known to be critical for both pituitary cell differentiation and gonadotropin subunit expression. By transient transfection and EMSA analysis, we demonstrate that GATA2 and GATA4 stimulate Adcyap1 promoter activity via a GATA cis-element located at position −191 in the rat Adcyap1 gene promoter. Furthermore, we show that addition of GATA2 or GATA4 significantly augments GnRH-mediated stimulation of Adcyap1 gene promoter activity in the gonadotrope LβT2 cell line. Conversely, blunting GATA expression with specific siRNA inhibits the ability of GnRH to stimulate ADCYAP1 mRNA levels in these cells. These data demonstrate a complex interaction between GnRH and GATA on ADCYAP1 expression, providing important new insights into the regulation of gonadotrope function.

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GATA transcription factors regulate LHβ gene expression

Cited by 31 publications

References 47 publications

Polygenic sex determination in the cichlid fish Astatotilapia burtoni

Polygenic sex determination in the cichlid fish Astatotilapia burtoni

cAMP-stimulated transcription of DGKθ requires steroidogenic factor 1 and sterol regulatory element binding protein 1

GATA augments GNRH-mediated increases in Adcyap1 gene expression in pituitary gonadotrope cells

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