Crimean-Congo Hemorrhagic Fever Virus, Mongolia, 2013–2014

Voorhees, Matthew A.; Padilla, Susana; Jamsransuren, Dulamjav; Koehler, Jeffrey W.; Delp, Korey L.; Adiyadorj, Dolgorkhand; Baasandagwa, Uyanga; Jigjav, Battsetseg; Olschner, Scott P; Minogue, Timothy D.; Schoepp, Randal J.

doi:10.3201/eid2412.180175

Cited by 17 publications

(13 citation statements)

References 22 publications

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“…Roughly 26% of Mongolia’s population of three million residents engage in traditional pastoral herding. This subset of the population is at an increased risk of exposure to both zoonotic and vector-borne infectious diseases [ 7 , 8 , 9 ]. Tick-borne rickettsioses in particular have a significant impact on the health of this at-risk population: peak tick bite rates occur during economically productive months (increased risk of disease), and low healthcare-seeking rates despite the presence of symptoms delay treatment [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

“…Characterization of Rickettsia species is also important due to serious clinical diseases in neighboring countries [ 16 , 17 , 18 , 19 , 20 , 21 ]. A previous study by our group collected and tested Dermacentor nuttalli and Hyalomma asiaticum tick pools for the presence of Crimean-Congo hemorrhagic fever virus by real-time RT-PCR [ 9 ]. Here, we utilized this same sample set to assess the presence and distribution of different Rickettsia spp.…”

Section: Introductionmentioning

confidence: 99%

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Molecular Characteristics of Rickettsia in Ticks Collected along the Southern Border of Mongolia

et al. 2020

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Tick-borne infections are a significant threat to public health, particularly in regions where individuals frequently enter tick habitats. Roughly 26% of the population in Mongolia practice nomadic pastoralism and are considered at high risk of exposure to ticks and the diseases they carry. This study tested ticks from Mongolia’s southern border for Rickettsia spp. to better understand the epidemiology of tick-borne diseases in the region. Dermacentor nuttalli and Hyalomma asiaticum ticks (n = 4022) were pooled and tested for Rickettsia spp. by real-time PCR. Melt-curve analyses and Sanger sequencing were used to identify Rickettsia species. Approximately 64% of the 786 tick pools tested positive for Rickettsia bacteria. Melt curve analyses identified four different Rickettsia species circulating in these tick pools. Amplicon sequencing of the ompA gene identified Rickettsia spp. that closely resembled R. raoultii and R. sibirica. Dermacentor nuttalli ticks from Govi-Altai had the highest maximum likelihood estimation infection rate 48.4% (95% CI: 41.7–56.5%), while Hyalommaasiaticum collected in Omnogovi had a rate of 7.6% (95% CI: 6.2–9.2%). The high detection of Rickettsia suggests a substantial risk of infection in southern Mongolia. Further studies are necessary to investigate the clinical burden of tick-borne diseases in Mongolia.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular Characteristics of Rickettsia in Ticks Collected along the Southern Border of Mongolia

et al. 2020

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“…Mongolia, a vast landlocked country, has up to 26% of the three million residents engaging in traditional pastoral herding. This subset of the population is at an increased risk of exposure to zoonotic and vectorborne infectious diseases [7][8][9]. Tick-borne rickettsioses can have a signi cant impact on health and livelihood within this at-risk population, with peak tick bite counts occurring during productive months paired with low healthcare seeking rates despite presence of symptoms [10].…”

Section: Introductionmentioning

confidence: 99%

“…Characterization of Rickettsia species is especially important, since they are implicated in serious clinical diseases in neighboring countries [16][17][18][19][20][21]. Previous studies by our group collected and tested Dermacentor nuttalli and Hyalomma asiaticum tick pools for the presence of Crimean-Congo hemorrhagic fever virus by real-time RT-PCR [9]. Here, we utilized this same sample set to assess the presence of different Rickettsia spp.…”

Section: Introductionmentioning

confidence: 99%

Molecular Characteristics of Rickettsia in Ticks Collected Along the Southern Border of Mongolia

Fricken

Voorhees

Koehler

et al. 2020

Preprint

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Introduction: Tick-borne infectious dDermacentor and iseases represent a significant threat to public health, particularly in regions where individuals frequently enter tick habitats. This is especially true in Mongolia, where 26% of the population are pastoral herders whose lifestyle results in high risk of tick-borne diseases, which include Rickettsial diseases. In this study, ticks collected along Mongolia’s southern border were tested for the presence of Rickettsia spp. DNA to better understand the risk of this bacterial infection in the region. Methods: Dermacentor and Hyalomma ticks (n = 4,022) collected across southern Mongolia (aimags Bayankhongor, Dornogovi, Govi-Altai, Khovd, and Omnogovi) were pooled and tested for Rickettsia spp. by real-time PCR. Subsequent melt-curve analyses and Sanger sequencing were used to identify specific Rickettsia species. Results: Approximately 64% of the 786 tick pools tested positive for Rickettsia bacteria. Melt curve analyses identified between four and six different Rickettsia species circulating in these tick pools. Amplicon sequencing of the ompA gene from selected positive samples identified Rickettsia spp. that closely resembled R. raoultii and R. sibirica. Detection rates varied greatly by sampling region and tick genus. Dermacentor ticks from the Govi-Altai region had the highest maximum likelihood estimation (MLE) infection rate of 48.4% (95% CI: 41.7-56.5%) while Hyalomma ticks collected in Omnogovi had an MLE rate of 7.6% (95% CI: 6.2-9.2%). Conclusions: Multiple Rickettsia species were found to circulate at high rates within native tick species in southern Mongolia. Further studies are required to investigate the clinical burden of disease associated with these Rickettsia spp. in exposed Mongolian populations.

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“…Dermacentor nuttalli (Acari: Ixodidate), is widely distributed across northern China, Russia and Mongolia [1], and is generally found in arid grassland areas suitable for grazing cattle and sheep [2], D. nuttalli responsible for spreading a variety of diseases, including spotted fever, tick-borne rickettsia, Crimeahemorrhagic fever and Babesiosis [2][3][4][5], in addition to being an important vector of Spirochetes and forest encephalitis [6,7]. Humans may experience symptoms similar to viral infections after being bitten by ticks, such as headaches, muscle pain, fevers, enlarged local lymph nodes or plaques, as well as severe liver and kidney damage and central nervous system damage [8,9].…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity Analysis of Dermacentor Nuttalli within Inner Mongolia, China

Zheng

Lin

Cai

et al. 2020

Preprint

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BackgroundTicks (Arthropoda, Ixodida), after mosquitoes, are the next most prevalent vector of infectious diseases; and are responsible for spreading a multitude of pathogens and threatening the health and welfare of animals and human beings. Yet, given the history of tick-borne pathogen infections in the Inner Mongolia Autonomous region of China, neither the genetic diversity nor the spatial distribution of haplotypes within ticks has been studied. MethodsWe characterized the haplotype distribution of Dermacentor nuttalli in four main pastoral areas of the Inner Mongolia Autonomous region, by sampling 109 individuals (recovered from sheep) in April-August 2019. The 16S rRNA gene, Cytochrome c Oxidase Subunit Ⅰ, and the Internal Transcribed Spacer 2 region were amplified and sequenced from extracted DNA.Results 82 haplotypes were identified, the most prevalent of which was H32. 12 sequences (11 of all sequences) represented the most abundant haplotypes, with a highly scattered distribution. Total haplotype diversity was 0.98318, while total nucleotide diversity was 0.11369. Neutrality tests revealed negative results in the four locations analyzed, which is indicative of an excess of recently derived haplotypes. Fixation index values (FST) indicate that the degree of genetic differentiation amongst some sampled populations were small, while others were moderate. Conclusion The genetic diversity of D.nuttalli populations in our region can likely adapt to different geographical environments, thereby leading to genetic diversity, and creating genetic differentiation amongst different populations. However, genetic differentiation is cryptic and does not form the pedigree geographical structure.

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Crimean-Congo Hemorrhagic Fever Virus, Mongolia, 2013–2014

Cited by 17 publications

References 22 publications

Molecular Characteristics of Rickettsia in Ticks Collected along the Southern Border of Mongolia

Molecular Characteristics of Rickettsia in Ticks Collected along the Southern Border of Mongolia

Molecular Characteristics of Rickettsia in Ticks Collected Along the Southern Border of Mongolia

Genetic Diversity Analysis of Dermacentor Nuttalli within Inner Mongolia, China

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