CRISP1 as a novel CatSper regulator that modulates sperm motility and orientation during fertilization

Ernesto, Juan; Muñoz, Mariana Weigel; Battistone, María Agustina; Vasen, Gustavo; Martínez-López, Pablo; Orta, Gerardo; Figueiras-Fierro, D.; Vega-Beltrán, José Luis de la; Moreno, Ignacio A.; Guidobaldi, Héctor Alejandro; Giojalas, Laura Cecilia; Darszon, Alberto; Cohen, Débora J.; Cuasnicú, Patricia S.

doi:10.1083/jcb.201412041

Cited by 85 publications

(110 citation statements)

References 77 publications

(146 reference statements)

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“…However, there has been little research in relation to the pathogenic mechanism of Cd, limited by the difficulty of recording the sperm membrane current, which reflects the channel activity. Here, we agree with Zhou’s opinion that cadmium was likely to affect sperm motility through the level of calcium entry, altered by calcium channel properties [44-47]. However, we believe CatSper, which is located in the principal piece of the sperm flagellum, was the foremost binding site of cadmium, causing toxicity.…”

Section: Discussionsupporting

confidence: 29%

Exposure to Cadmium Impairs Sperm Functions by Reducing CatSper in Mice

Wang

Chang

Peng

et al. 2017

Cell Physiol Biochem

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Background: Cadmium (Cd), a common environmental heavy metal and endocrine disruptor, is known to exert toxic effects on the testes. However, the mechanisms accounting for its toxicity in mature spermatozoa remain unclear. Methods: Adult male C57BL/6 mice were orally administered with CdCl₂ for 5 weeks at 3 mg·kg^-1·day^-1. Additionally, mouse spermatozoa were incubated in vitro with different doses of CdCl₂ (0, 10, 50, 250 µM). Several sperm functions including the sperm motility, viability and acrosome reaction (AR) ratio were then examined. Furthermore, the current and expression levels of both the sperm-specific Ca²⁺ channel (CatSper) and the sperm-specific K⁺ channel (KSper) were evaluated by patch-clamping and western blotting, respectively. Results: Our data showed that the motility, viability and AR of sperm exposed to cadmium significantly decreased in vivo and in vitro. Interestingly, these changes were correlated with changes in CatSper but not KSper. Conclusion: The findings indicate sperm dysfunction during both chronic and acute cadmium exposure as well as a specific role for CatSper in the reproductive toxicity of cadmium.

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Section: Discussionsupporting

confidence: 29%

Exposure to Cadmium Impairs Sperm Functions by Reducing CatSper in Mice

Wang

Chang

Peng

et al. 2017

Cell Physiol Biochem

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“…And (iv) what molecule(s) guide the acrosome‐reacted spermatozoon to the oocyte‐cumulus complex? A chemotactic response toward CRISP1, a protein secreted by the cumulus cells, was observed in the mouse (Ernesto et al, ), so other molecules released by the cumulus layer may chemically attract sperm toward the egg.…”

Section: Discussionmentioning

confidence: 99%

An intact acrosome is required for the chemotactic response to progesterone in mouse spermatozoa

Guidobaldi

Hirohashi

Cubilla

et al. 2017

Molecular Reproduction Devel

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Mammalian sperm become fertilization-competent in the oviduct, during a process known as capacitation that involves the acquisition of the ability to exocytose the acrosome but also the chemotactic responses – both of which contribute to successful fertilization. Chemotaxis is used by spermatozoa to orient and to locate the egg; the acrosome reaction facilitates sperm binding to and fusing with the egg membrane. Mammalian spermatozoa are able to sense picomolar concentrations of progesterone, which drives chemotactic behavior. The state of the acrosome during the chemotactic response, however, is unknown. Genetically modified mouse spermatozoa were employed in a chemotaxis assay under fluorescence microscopy to evaluate the acrosome status while swimming, allowing us to elucidate the acrosome integrity of sperm responding to progesterone-induced chemotaxis. We first showed that wild-type mouse spermatozoa chemotactically respond to a gradient of progesterone, and that the genetic modifications employed do not affect the chemotactic behavior of sperm to progesterone. Next, we found that acrosome-intact, but not acrosome-reacted, spermatozoa orient and respond to picomolar concentrations of progesterone and that chemotaxis normally occurs prior to the acrosome reaction. Our results suggest that premature commitment to acrosome exocytosis leads to navigation failure, so proper control and timing of the acrosome reaction is required for fertilization success and male fertility.

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“…Reptile CRISPs have been reported to regulate a range of ion channel types and in vitro and in vivo methods identified mouse CRISP4 as an inhibitor of transient receptor potential M8 channel (TRPM8) activity [85]. Moreover, mouse CRISP1 was recently reported to be capable of modulating the principal sperm Ca 2+ channel CatSper involved in hyperactivation [86], supporting the idea that CRISPs act as endogenous protein ion channel regulators.…”

Section: Gamete Fusion (Plasmogamy)mentioning

confidence: 98%

Fertilization Mechanisms in Flowering Plants

2016

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Compared to the animal kingdom, fertilization is particularly complex in flowering plants (angiosperms). Sperm cells of angiosperms have lost their motility and require transportation as a passive cargo by the pollen tube cell to the egg apparatus (egg cell and accessory synergid cells). Sperm cell release from the pollen tube occurs after intensive communication between the pollen tube cell and the receptive synergid, culminating in the lysis of both interaction partners. Following release of the two sperm cells they interact and fuse with two dimorphic female gametes (egg and central cell) forming the major seed components embryo and endosperm, respectively. This process is known as double fertilization. Here we review the current understanding of the processes of sperm cell reception, gamete interaction, their pre-fertilization activation and fusion as well as the mechanisms plants use to prevent the fusion of egg cells with multiple sperm cells. The role of Ca2+ is highlighted in these various processes and comparisons are drawn between fertilization mechanisms in flowering plants and other eukaryotes including mammals.

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CRISP1 as a novel CatSper regulator that modulates sperm motility and orientation during fertilization

Abstract: CRISP1 is expressed by cumulus cells and plays a role in fertilization by modulating sperm orientation, hyperactivation, and key Ca2+ channels in sperm.

Cited by 85 publications

References 77 publications

Exposure to Cadmium Impairs Sperm Functions by Reducing CatSper in Mice

Exposure to Cadmium Impairs Sperm Functions by Reducing CatSper in Mice

An intact acrosome is required for the chemotactic response to progesterone in mouse spermatozoa

Fertilization Mechanisms in Flowering Plants

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