CRISPR-based genetic control strategies for insect pests

Yan, Ying; Aumann, Roswitha A.; Häcker, Irina; Schetelig, Marc F.

doi:10.1016/j.jia.2022.11.003

Cited by 20 publications

(9 citation statements)

References 146 publications

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“…So far, gene editing has been used mostly to test hypotheses about gene function (see above). But gene editing could in principle also be used in practical insect pest control as a means of introducing fertility-limiting genes into the pest population through the sterile insect technique (SIT) ( 204 , 205 ).…”

Section: Male Reproduction As a Target For Lepidopteran Pest Controlmentioning

confidence: 99%

“…As described in section 9, gene editing using CRISPR/Cas9 has already proved of great value in experimental investigations of gene function in insects and is an obvious choice to use in constructing genetic sexing lines for SIT in Lepidoptera. Reflecting the considerable interest in using the CRISPR-Cas9 technique in pest control ( 205 ), several recent publications have reviewed its application to lepidopteran pests ( 204 , 215 – 217 ). Table 3 lists several examples.…”

Section: Male Reproduction As a Target For Lepidopteran Pest Controlmentioning

confidence: 99%

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Dichotomous sperm in Lepidopteran insects: a biorational target for pest management

Seth,

Yadav,

Reynolds

2023

Front. Insect Sci.

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Lepidoptera are unusual in possessing two distinct kinds of sperm, regular nucleated (eupyrene) sperm and anucleate (apyrene) sperm (‘parasperm’). Sperm of both types are transferred to the female and are required for male fertility. Apyrene sperm play ‘helper’ roles, assisting eupyrene sperm to gain access to unfertilized eggs and influencing the reproductive behavior of mated female moths. Sperm development and behavior are promising targets for environmentally safer, target-specific biorational control strategies in lepidopteran pest insects. Sperm dimorphism provides a wide window in which to manipulate sperm functionality and dynamics, thereby impairing the reproductive fitness of pest species. Opportunities to interfere with spermatozoa are available not only while sperm are still in the male (before copulation), but also in the female (after copulation, when sperm are still in the male-provided spermatophore, or during storage in the female’s spermatheca). Biomolecular technologies like RNAi, miRNAs and CRISPR-Cas9 are promising strategies to achieve lepidopteran pest control by targeting genes directly or indirectly involved in dichotomous sperm production, function, or persistence.

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Section: Male Reproduction As a Target For Lepidopteran Pest Controlmentioning

confidence: 99%

Section: Male Reproduction As a Target For Lepidopteran Pest Controlmentioning

confidence: 99%

Dichotomous sperm in Lepidopteran insects: a biorational target for pest management

Seth,

Yadav,

Reynolds

2023

Front. Insect Sci.

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“…These findings are timely since the technological and financial hurdles to developing new methods to sterilized vertebrates may diminish in coming years 41 – 43 . Sea lamprey are currently chemosterilized in a contained laboratory using highly specialized protective equipment 20 , which limits the geographic scope of its deployment, increases labor, facility, and animal transportation costs, and applicability to other pests.…”

Section: Discussionmentioning

confidence: 97%

“…While these ideas are not new 11 , they have often been overlooked in www.nature.com/scientificreports/ sea lamprey control during the past 20 years 12 -meanwhile being deployed and shown effective for insect pests such as the Mediterranean fruit fly, screw worm, and tsetse flies 40 . These findings are timely since the technological and financial hurdles to developing new methods to sterilized vertebrates may diminish in coming years [41][42][43] . Sea lamprey are currently chemosterilized in a contained laboratory using highly specialized protective equipment 20 , which limits the geographic scope of its deployment, increases labor, facility, and animal transportation costs, and applicability to other pests.…”

Section: Discussionmentioning

confidence: 97%

A decade-long study demonstrates that a population of invasive sea lamprey (Petromyzon marinus) can be controlled by introducing sterilized males

Johnson,

Lewandoski,

Jubar

et al. 2024

Sci Rep

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The release of sterilized insects to control pest populations has been used successfully during the past 6 decades, but application of the method in vertebrates has largely been overlooked or met with failure. Here, we demonstrate for the first time in fish, that a small population of sea lamprey (Petromyzon marinus; Class Agnatha), arguably one of the most impactful invasive fish in the world, can be controlled by the release of sterilized males. Specifically, the release of high numbers of sterile males (~ 1000's) into a geographically isolated population of adult sea lamprey resulted in the first multiyear delay in pesticide treatment since treatments began during 1966. Estimates of percent reduction in recruitment of age-1 sea lamprey due to sterile male release ranged from 7 to 99.9% with the precision of the estimate being low because of substantial year-to-year variability in larval density and distribution. Additional monitoring that accounts for recruitment variability in time and space would reduce uncertainty in the degree to which sterile male release reduces recruitment rates. The results are relevant to vertebrate pest control programs worldwide, especially as technical opportunities to sterilize vertebrates and manipulate sex ratios expand.

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“…Among the three types of CRISPR systems, Type II is the most extensively studied. The system includes the Cas9 enzyme, a cluster of crRNA that encodes guide RNAs, and an accompanying tracrRNA that assists in breaking down the crRNA cluster into distinct units [2]. By expressing human designed Cas9 enzyme and the necessary RNA components, CRISPR-Cas reconstitutes the RNA-guided nuclease function in mammalian cells.…”

Section: Crispr/cas Systemmentioning

confidence: 99%

CRISPR/Cas system: A powerful tool for de-extinction

Shen

2023

TNS

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The rapid advances in CRISPR technology have opened up new avenues of research and provided hope for a future where extinct species can be brought back to life. De-extinction, the process of resurrecting extinct species by using genetic engineering techniques, is one potential application of CRISPR technology that has gained increasing attention in recent years. The idea is to recover the genetic information of extinct species from preserved tissue samples and recreate them using modern genetic engineering techniques. George Church, a geneticist at Harvard University, is one of the leading scientists exploring the potential of CRISPR for de-extinction. He has spearheaded efforts to resurrect the woolly mammoth, which went extinct over 4,000 years ago, by inserting mammoth DNA into the genome of the Asian elephant. While this project is still in its early stages, it has sparked renewed interest in using CRISPR to bring back other extinct species, such as the Pyrenean ibex, the passenger pigeon, and the Tasmanian tiger. This review paper aims to explore the potential of CRISP technology for de-extinction, including its technical and ethical challenges, and the progress that has been made in various de-extinction projects involving CRISPR technology.

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CRISPR-based genetic control strategies for insect pests

Cited by 20 publications

References 146 publications

Dichotomous sperm in Lepidopteran insects: a biorational target for pest management

Dichotomous sperm in Lepidopteran insects: a biorational target for pest management

A decade-long study demonstrates that a population of invasive sea lamprey (Petromyzon marinus) can be controlled by introducing sterilized males

CRISPR/Cas system: A powerful tool for de-extinction

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