CRISPR/Cas9‐mediated gene editing of <i>GmJAGGED1</i> increased yield in the low‐latitude soybean variety Huachun 6

Cai, Zhandong; Xian, Peiqi; Cheng, Yanbo; Ma, Qibin; Lian, Tengxiang; Nian, Hai; Ge, Liangfa

doi:10.1111/pbi.13673

Cited by 32 publications

(19 citation statements)

References 6 publications

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“…GmJAG in low-latitude variety Huachun 6 was edited by CRISPR/Cas9-based genome-editing technology, the offspring showed the same phenotype as that of ln mutant which just exists in the high latitude soybean varieties. Furthermore, the yield of gmjag was higher than Huachun 6 ( Cai et al, 2021 ). Another gene that can regulate the narrow-leaf phenotype of soybean is CTP ( Chicken Toes-Like Leaf and Petalody Flower ).…”

Section: Leaf Shape and Sizementioning

confidence: 81%

“…Ln is a homologous gene of Arabidopsis JAGGED ( JAG ), which regulates lateral organ development and the number of seeds per pod (NSPP) ( Jeong et al, 2013 ). Interestingly, ln widely exists in the high latitude soybean varieties, but it does not exist in the low latitude soybean varieties and is hardly found in wild soybean ( Cai et al, 2021 ). GmJAG in low-latitude variety Huachun 6 was edited by CRISPR/Cas9-based genome-editing technology, the offspring showed the same phenotype as that of ln mutant which just exists in the high latitude soybean varieties.…”

Section: Leaf Shape and Sizementioning

confidence: 99%

“…Furthermore, soybean populations are geographically structured, and some agronomic traits show geographic distribution patterns ( Zhou et al, 2015 ). For example, soybean varieties from northern China tend to exhibit narrow leaf shapes, while varieties from southern China exhibit ovate leaf shapes ( Zhou et al, 2015 ; Cai et al, 2021 ). The key regulatory gene responsible for this phenomenon may be Ln , which was not found in 383 re-sequenced accessions collected from low latitudes ( Cai et al, 2021 ).…”

Section: Organ Trait Selection During the Domestication Process Of So...mentioning

confidence: 99%

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The Organ Size and Morphological Change During the Domestication Process of Soybean

Zhou

Wang²,

Mao³

et al. 2022

Front. Plant Sci.

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Soybean is one of the most important legume crops that can provide the rich source of protein and oil for human beings and livestock. In the twenty-one century, the total production of soybean is seriously behind the needs of a growing world population. Cultivated soybean [Glycine max (L.) Merr.] was domesticated from wild soybean (G. soja Sieb. and Zucc.) with the significant morphology and organ size changes in China around 5,000 years ago, including twisted stems to erect stems, small seeds to large seeds. Then it was spread worldwide to become one of the most popular and important crops. The release of the reference soybean genome and omics data provides powerful tools for researchers and breeders to dissect the functional genes and apply the germplasm in their work. Here, we summarized the function genes related to yield traits and organ size in soybean, including stem growth habit, leaf size and shape, seed size and weight. In addition, we also summarized the selection of organ traits during soybean domestication. In the end, we also discussed the application of new technology including the gene editing on the basic research and breeding of soybean, and the challenges and research hotspots in the future.

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Section: Leaf Shape and Sizementioning

confidence: 81%

Section: Leaf Shape and Sizementioning

confidence: 99%

Section: Organ Trait Selection During the Domestication Process Of So...mentioning

confidence: 99%

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The Organ Size and Morphological Change During the Domestication Process of Soybean

Zhou

Wang²,

Mao³

et al. 2022

Front. Plant Sci.

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“…Knockout of FAD2 genes by CRISPR/Cas9 leads to increases in oleic acid and total monounsaturated fatty acid composition with concurrent decreases in undesirable polyunsaturated linoleic and linolenic fatty acid content [ 12 ]. CRISPR/Cas9-mediated gene editing of GmJAGGED1 increased yield in a low-latitude soybean variety [ 13 ]. With the help of genome editing, a remarkable work to domesticate wild allotetraploid rice de novo into a new staple cereal has been reported recently.…”

Section: Introductionmentioning

confidence: 99%

Epigenome and Epitranscriptome: Potential Resources for Crop Improvement

Hou

Wan

2021

IJMS

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Crop breeding faces the challenge of increasing food demand, especially under climatic changes. Conventional breeding has relied on genetic diversity by combining alleles to obtain desired traits. In recent years, research on epigenetics and epitranscriptomics has shown that epigenetic and epitranscriptomic diversity provides additional sources for crop breeding and harnessing epigenetic and epitranscriptomic regulation through biotechnologies has great potential for crop improvement. Here, we review epigenome and epitranscriptome variations during plant development and in response to environmental stress as well as the available sources for epiallele formation. We also discuss the possible strategies for applying epialleles and epitranscriptome engineering in crop breeding.

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“… Jeong et al (2012) cloned a soybean locus ln and proved its pleiotropy for narrow-leaf and higher seed number. Cai et al (2021) used CRISPR/Cas9 technology to edit gene Ln and its homologous genes in soybean cultivar “Huachun 6” to create a new material carrying gene ln , which is available for the future field breeding. To confirm whether the difference of SNPP between Ln and ln genotypes is caused by ovule number per pistil, Fang et al (2013) found that ovate leaflet cultivar “Han 2296” with two-seeded pods has two to three ovules per ovary, and narrow leaflet cultivar “Lvbaoshi” with four-seeded pods has three to four ovules per ovary.…”

Section: Introductionmentioning

confidence: 99%

Integrated Bioinformatics Analyses of PIN1, CKX, and Yield-Related Genes Reveals the Molecular Mechanisms for the Difference of Seed Number Per Pod Between Soybean and Cowpea

et al. 2021

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There is limited advancement on seed number per pod (SNPP) in soybean breeding, resulting in low yield in China. To address this issue, we identified PIN1 and CKX gene families that regulate SNPP in Arabidopsis, analyzed the differences of auxin and cytokinin pathways, and constructed interaction networks on PIN1, CKX, and yield-related genes in soybean and cowpea. First, the relative expression level (REL) of PIN1 and the plasma membrane localization and phosphorylation levels of PIN1 protein were less in soybean than in cowpea, which make auxin transport efficiency lower in soybean, and its two interacted proteins might be involved in serine hydrolysis, so soybean has lower SNPP than cowpea. Then, the CKX gene family, along with its positive regulatory factor ROCK1, had higher REL and less miRNA regulation in soybean flowers than in cowpea ones. These lead to higher cytokinin degradation level, which further reduces the REL of PIN1 and decreases soybean SNPP. We found that VuACX4 had much higher REL than GmACX4, although the two genes essential in embryo development interact with the CKX gene family. Next, a tandem duplication experienced by legumes led to the differentiation of CKX3 into CKX3a and CKX3b, in which CKX3a is a key gene affecting ovule number. Finally, in the yield-related gene networks, three cowpea CBP genes had higher RELs than two soybean CBP genes, low RELs of three soybean-specific IPT genes might lead to a decrease in cytokinin synthesis, and some negative and positive SNPP regulation were found, respectively, in soybean and cowpea. These networks may explain the SNPP difference in the two crops. We deduced that ckx3a or ckx3a ckx6 ckx7 mutants, interfering CYP88A, and over-expressed DELLA increase SNPP in soybean. This study reveals the molecular mechanism for the SNPP difference in the two crops, and provides an important idea for increasing soybean yield.

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CRISPR/Cas9‐mediated gene editing of GmJAGGED1 increased yield in the low‐latitude soybean variety Huachun 6

Cited by 32 publications

References 6 publications

The Organ Size and Morphological Change During the Domestication Process of Soybean

The Organ Size and Morphological Change During the Domestication Process of Soybean

Epigenome and Epitranscriptome: Potential Resources for Crop Improvement

Integrated Bioinformatics Analyses of PIN1, CKX, and Yield-Related Genes Reveals the Molecular Mechanisms for the Difference of Seed Number Per Pod Between Soybean and Cowpea

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