CRISPR/Cas9-mediated mutagenesis of the dihydroflavonol-4-reductase-B (DFR-B) locus in the Japanese morning glory Ipomoea (Pharbitis) nil

Watanabe, Kenta; Kobayashi, Anna; Endo, Masaki; Sage-Ono, Kimiyo; Toki, Seiichi; Ono, Michiyuki

doi:10.1038/s41598-017-10715-1

Cited by 122 publications

(62 citation statements)

References 55 publications

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“…Considering the ease of use of the new genome editing tools 64 and especially of the CRISPR/Cas9 system 65 , more and more transformed material will be available. To our knowledge, there are five scientific publications related to successful genome editing of ornamental plants with trait of commercial interest, including ipomoea [66][67][68] , petunia 69 , and torenia 70 . CRISPR/Cas9-mediated mutagenesis of the EPHEMERAL1 locus delayed petal senescence in transformed ipomoea 66 .…”

Section: Resultsmentioning

confidence: 99%

“…CRISPR/Cas9-mediated mutagenesis of the EPHEMERAL1 locus delayed petal senescence in transformed ipomoea 66 . Flower color changes was induced using CRISPR/Cas9 technology 67,68,70 . As an example, ipomoea with pale-yellow petals was produced for the first time by successful knockout of InCCD4, involved in carotenoid degradation, using CRISP/Cas9 system 68 .…”

Section: Resultsmentioning

confidence: 99%

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Overview and detectability of the genetic modifications in ornamental plants

Boutigny¹,

Dohin²,

Pornin³

et al. 2020

Hortic Res

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The market of ornamental plants is extremely competitive, and for many species genetic engineering can be used to introduce original traits of high commercial interest. However, very few genetically modified (GM) ornamental varieties have reached the market so far. Indeed, the authorization process required for such plants has a strong impact on the profitability of the development of such products. Considering the numerous scientific studies using genetic modification on ornamental species of interest, a lot of transformed material has been produced, could be of commercial interest and could therefore be unintentionally released on the market. The unintentional use of GM petunia in breeding programs has indeed recently been observed. This review lists scientific publications using GM ornamental plants and tries to identify whether these plants could be detected by molecular biology tools commonly used by control laboratories.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Overview and detectability of the genetic modifications in ornamental plants

Boutigny¹,

Dohin²,

Pornin³

et al. 2020

Hortic Res

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“…Recent studies reported that high-quality genome sequence (Hoshino et al 2016) and the success of targeted mutagenesis by CRISPR/Cas9 system (Watanabe et al 2017) in I. nil. These information and technology would enable advanced carotenoid metabolic engineering so more progressive studies using I. nil will be conducted.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of carotenogenic genes in the Japanese morning glory Ipomoea (Pharbitis) nil

Watanabe

Oda-Yamamizo

Sage-Ono

et al. 2017

Plant Biotechnology

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Japanese morning glory, Ipomoea nil, has several coloured flowers except yellow, because it can accumulate only trace amounts of carotenoids in the petal. To make the petal yellow with carotenoids, we introduced five carotenogenic genes (geranylgeranyl pyrophosphate synthase, phytoene synthase, lycopene β-cyclase and β-ring hydroxylase from Ipomoea obscura var. lutea and bacterial phytoene desaturase from Pantoea ananatis) to white-flowered I. nil cv. AK77 with a petal-specific promoter by Rhizobium (Agrobacterium)-mediated transformation method. We succeeded to produce transgenic plants overexpressing carotenogenic genes. In the petal of the transgenic plants, mRNA levels of the carotenogenic genes were 10 to 1,000 times higher than those of non-transgenic control. The petal colour did not change visually; however, carotenoid concentration in the petal was increased up to about ten-fold relative to non-transgenic control. Moreover, the components of carotenoids in the petal were diversified, in particular, several β-carotene derivatives, such as zeaxanthin and neoxanthin, were newly synthesized. This is the first report, to our knowledge, of changing the component and increasing the amount of carotenoid in petals that lack ability to biosynthesize carotenoids.

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“…Overexpression of FT has been used in many plant species to induce advanced flowering [120,121], thus enabling a more rapid and refined approach to breeding. CRISPR/Cas has also been used successfully to target dihydroflavonol-4-reductase-B (DFR-B), encoding an anthocyanin biosynthesis enzyme that is responsible for the color of the plant's stems, leaves, and flowers [122]. Moreover, CRISPR/Cas9 system was employed to specifically induce targeted mutagenesis of GmFT2a, an integrator in the photoperiod flowering pathway in soybean [123].…”

Section: Use Of Gened Tools For Growth Yield Fiber and Seed Qualitmentioning

confidence: 99%

Targeted Genome Editing for Cotton Improvement

Khan¹,

Khan²,

Mubarik³

et al. 2018

Past, Present and Future Trends in Cotton Breeding

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Conventional tools induce mutations randomly throughout the cotton genome-making breeding difficult and challenging. During the last decade, progress has been made to edit the gene of interest in a very precise manner. Targeted genome engineering with engineered nucleases (ENs) specifically zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeat (CRISPR) RNA-guided nucleases (e.g., Cas9) has been described as a "game-changing technology" for diverse fields as human genetics and plant biotechnology. In eukaryotic systems, ENs create double-strand breaks (DSBs) at the targeted DNA sequence which are repaired by nonhomologous end joining (NHEJ) or homologydirected recombination (HDR) mechanisms. ENs have been used successfully for targeted mutagenesis, gene knockout, and multisite genome editing (GenEd) in model plants and crop plants such as cotton, rice, and wheat. Recently, cotton genome has also been edited for targeted mutagenesis through CRISPR/Cas for improved lateral root formation. In addition, an efficient and fast method has been developed to evaluate guide RNAs transiently in cotton. The targeted disruption of undesirable genes or metabolic pathway can be achieved to increase quality of cotton. Undesirable metabolites like gossypol in cottonseed can be targeted efficiently using ENs for seed-specific low-gossypol cotton. Moreover, ENs are also helpful in gene stacking for herbicide resistance, insect resistance, and abiotic stress tolerance.

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CRISPR/Cas9-mediated mutagenesis of the dihydroflavonol-4-reductase-B (DFR-B) locus in the Japanese morning glory Ipomoea (Pharbitis) nil

Cited by 122 publications

References 55 publications

Overview and detectability of the genetic modifications in ornamental plants

Overview and detectability of the genetic modifications in ornamental plants

Overexpression of carotenogenic genes in the Japanese morning glory <i>Ipomoea</i> (<i>Pharbitis</i>) <i>nil</i>

Targeted Genome Editing for Cotton Improvement

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