CRISPR-mediated knockout of nicotinic acetylcholine receptor (nAChR) α6 subunit confers high levels of resistance to spinosyns in Spodoptera frugiperda

Shi, Tailong; Tang, Ping; Wang, Xingliang; Yang, Yihua; Wu, Yingliang

doi:10.1016/j.pestbp.2022.105191

Cited by 10 publications

(7 citation statements)

References 40 publications

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“…Emamectin benzoate also had a relative high toxicity compared to the 4 other insecticides evaluated here. The toxicity of emamectin benzoate is similar to that of broflanilide and spinetoram (Shi et al 2022, Wang et al 2022b), indicating that these 3 insecticides could be highly effective for suppressing S. frugiperda populations in China. The toxicity of chlorantraniliprole, chlorfenapyr, spinosad, indoxacarb was lower than emamectin benzoate, but still greater than for many older insecticides such as pyrethroids (Zhao et al 2020, Wang et al 2023).…”

Section: Discussionmentioning

confidence: 87%

Susceptibility and diagnostic concentration for Bacillus thuringiensis toxins and newer chemical insecticides in Spodoptera frugiperda (Lepidoptera: Noctuidae) from China

Guan,

Zhang,

Lin

et al. 2023

Journal of Economic Entomology

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The fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae), is a cosmopolitan pest that exploits more than 350 host plants, including economically important crops such as corn, cotton and rice. Control of S. frugiperda largely relies on transgenic crops producing insecticidal proteins from Bacillus thuringiensis (Bt) and spraying synthetic insecticides. Here, we established the susceptibility and diagnostic concentration for 2 Bt toxins and 5 newer insecticides in invasive populations of S. frugiperda from southeastern China. Concentrations causing 50% mortality (LC50) in ten field populations sampled in 2022 ranged from 2.13 to 19.29 and 22.43 to 71.12 ng/cm2 for Cry1Fa and Vip3Aa, and 0.83 to 5.30, 2.83 to 9.94, 0.04 to 0.23, 4.59 to 8.40, and 1.49 to 6.79 mg/liter for chlorantraniliprole, chlorfenapyr, emamectin benzoate, indoxacarb, and spinosad, respectively. Relative to the susceptible strain YJ-19, the largest resistance ratio in the field populations was 5.1, 1.6, 6.2, 3.9, 4.6, 2.2, and 3.6 for Cry1Fa, Vip3Aa, chlorantraniliprole, chlorfenapyr, emamectin benzoate, indoxacarb, and spinosad, respectively, indicating that the field populations were generally susceptible to these Bt toxins and insecticides. Based on the pooled response of the field populations, the diagnostic concentration for resistance monitoring, estimated as ca. twice the LC99, was 400 and 1,500 ng/cm2 for Cry1Fa and Vip3Aa, and 2, 40, 60, 60, and 100 mg/liter for emamectin benzoate, chlorantraniliprole, chlorfenapyr, spinosad, and indoxacarb, respectively. These results provide useful information for monitoring resistance to key Bt toxins and insecticides for the control of S. frugiperda in China.

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Section: Discussionmentioning

confidence: 87%

Susceptibility and diagnostic concentration for Bacillus thuringiensis toxins and newer chemical insecticides in Spodoptera frugiperda (Lepidoptera: Noctuidae) from China

Guan,

Zhang,

Lin

et al. 2023

Journal of Economic Entomology

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“… †† The Sf α 6‐KO strain was produced by knocking out the nAChRα6 gene in the YJ‐19 strain, which conferred 307‐ and 517‐fold resistance to spinosad and spinetoram (IRAC MoA Group 5), respectively (Shi et al. , 2022). …”

Section: Resultsmentioning

confidence: 99%

“…The 4946E, 4790M, and 4790K strains of P. xylostella were generated by introgression of PxRyR mutations (G4946E, I4790M, or I4790K) into the IPP-S strain, which conferred from 16-fold to more than 2000-fold resistance to diamides (IRAC MoA Group 28) (Jiang et al, 2021). † † The Sfα6-KO strain was produced by knocking out the nAChRα6 gene in the YJ-19 strain, which conferred 307-and 517-fold resistance to spinosad and spinetoram (IRAC MoA Group 5), respectively (Shi et al, 2022). P. xylostella, and S. frugiperda.…”

Section: Discussionmentioning

confidence: 99%

“…For each field-sampled population, more than 30 egg masses and/or 200 larvae were collected and reared to adulthood. For assessing cross-resistance, we used the spinosyns-resistant strain Sfα6-KO that was produced by CRISPR-mediated knockout of the nAChRα6 gene in YJ-19 (Shi et al, 2022). Larvae were reared on an artificial diet with soybean flour and wheatgerm as major ingredients (Shen & Wu, 1995) and adults were supplied with 10% sugar water during oviposition on cheesecloth.…”

Section: Insectsmentioning

confidence: 99%

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Baseline susceptibility of Helicoverpa armigera, Plutella xylostella, and Spodoptera frugiperda to the meta‐diamide insecticide broflanilide

et al. 2022

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Broflanilide is a novel meta-diamide insecticide that acts as a γ -aminobutyric acid-gated chloride channel allosteric modulator. With its unique mode of action, broflanilide has no known cross-resistance with existing insecticides and is expected to be an effective tool for the management of insecticide resistance. Establishing the baseline susceptibility to this insecticide is an essential step for developing and implementing effective resistance management strategies. Here we evaluated the baseline susceptibility to broflanilide for 3 cosmopolitan lepidopteran pest species, Helicoverpa armigera, Plutella xylostella, and Spodoptera frugiperda. Broflanilide exhibited high activity against populations sampled in the major distribution range of these pests in China, with median lethal concentrations (LC 50 ) ranging between 0.209 and 0.684, 0.076 and 0.336, and 0.075 and 0.219 mg/L for H. armigera, P. xylostella, and S. frugiperda, respectively. Amongpopulation variability in susceptibility to broflanilide was moderate for H. armigera (3.3fold), P. xylostella (4.4-fold), and S. frugiperda (2.9-fold). The recommended diagnostic concentrations for H. armigera, P. xylostella, and S. frugiperda were 8, 4, and 2 mg/L, respectively. Little or no cross-resistance to broflanilide was detected in 3 diamide-resistant strains of P. xylostella and 1 spinosyns-resistant strain of S. frugiperda. Our results provide critical information for the development of effective resistance management programs to sustain efficacy of broflanilide against these key lepidopteran pests.

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“…The two wild-type strains used were the WH-S S. exigua strain, which was originally collected from Wuhan (Hubei, China) in 1998 and provided to us by the Wuhan Institute of Vegetables ( 68 ), and the YJ-19 S. frugiperda strain, which we collected from maize in Yuanjiang (Yunan, China) in 2019 ( 69 ). Both strains had been maintained in the laboratory without exposure to insecticides since their collection from the field.…”

Section: Methodsmentioning

confidence: 99%

Divergent amplifications of CYP9A cytochrome P450 genes provide two noctuid pests with differential protection against xenobiotics

Shi,

Liu,

et al. 2023

Proc. Natl. Acad. Sci. U.S.A.

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Here, we provide mechanistic support for the involvement of the CYP9A subfamily of cytochrome P450 monooxygenases in the detoxification of host plant defense compounds and chemical insecticides in Spodoptera exigua and Spodoptera frugiperda . Our comparative genomics shows that a large cluster of CYP9A genes occurs in the two species but with significant differences in its contents, including several species-specific duplicates and substantial sequence divergence, both between orthologs and between duplicates. Bioassays of CRISPR-Cas9 knockouts of the clusters show that, collectively, the CYP9As can detoxify two furanocoumarin plant defense compounds (imperatorin and xanthotoxin) and insecticides representing three different chemotypes (pyrethroids, avermectins, and oxadiazines). However, in vitro metabolic assays of heterologously expressed products of individual genes show several differences between the species in the particular CYP9As with activities against these compounds. We also find that the clusters show tight genetic linkage with high levels of pyrethroid resistance in field strains of the two species. We propose that their divergent amplifications of the CYP9A subfamily have not only contributed to the development of the broad host ranges of these species over long evolutionary timeframes but also supplied them with diverse genetic options for evolving resistance to chemical insecticides in the very recent past.

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CRISPR-mediated knockout of nicotinic acetylcholine receptor (nAChR) α6 subunit confers high levels of resistance to spinosyns in Spodoptera frugiperda

Cited by 10 publications

References 40 publications

Susceptibility and diagnostic concentration for Bacillus thuringiensis toxins and newer chemical insecticides in Spodoptera frugiperda (Lepidoptera: Noctuidae) from China

Susceptibility and diagnostic concentration for Bacillus thuringiensis toxins and newer chemical insecticides in Spodoptera frugiperda (Lepidoptera: Noctuidae) from China

Baseline susceptibility of Helicoverpa armigera, Plutella xylostella, and Spodoptera frugiperda to the meta‐diamide insecticide broflanilide

Divergent amplifications of CYP9A cytochrome P450 genes provide two noctuid pests with differential protection against xenobiotics

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