Critical comparison of performances of superficially porous particles and sub-2μm particles under optimized ultra-high pressure conditions

Zhang, Yuan; Wang, Xiaoli; Mukherjee, Partha; Petersson, Patrik

doi:10.1016/j.chroma.2009.03.071

Cited by 83 publications

(65 citation statements)

References 33 publications

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“…The total porosity of the Cortecs column (0.549) was (as expected) around 15-25% lower than the fully porous particle packed columns (range 0.635-0.721). This is similar to the observations of Zhang et al [23] and Liekens et al [14] when comparing HALO (core shell) and Acquity (fully porous) columns. Clearly, the Cortecs and TITAN columns have the lowest internal porosity ( int = 0.357 and 0.367 respectively), although the former (shell) column value was only slightly lower.…”

Section: Chemicals and Reagentssupporting

confidence: 81%

Comparison of the kinetic performance and retentivity of sub-2μm core–shell, hybrid and conventional bare silica phases in hydrophilic interaction chromatography

Heaton

McCalley

2014

Journal of Chromatography A

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Section: Chemicals and Reagentssupporting

confidence: 81%

Comparison of the kinetic performance and retentivity of sub-2μm core–shell, hybrid and conventional bare silica phases in hydrophilic interaction chromatography

Heaton

McCalley

2014

Journal of Chromatography A

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“…To this end, various techniques have been introduced, including the use of sub-2 μm particles, high temperature, monolithic columns and superfi cially porous stationary phases (Carr et al, 2009;Zhang et al, 2009;Poppe, 1997;Plumb et al, 2007;McNeff et al, 2007;Fekete et al, 2010;Guillarme et al, 2009). The term UHPLC, which stands for ultra high-performance liquid chromatography, may be used to indicate any one of these techniques.…”

Section: Chromatographymentioning

confidence: 99%

Systematic LC‐MS/MS bioanalytical method development that incorporates plasma phospholipids risk avoidance, usage of incurred sample and well thought‐out chromatography

Jemal

Ouyang

Xia

2009

Biomedical Chromatography

184

124

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This treatise summarizes the underlying principle and the road map for systematic LC-MS/MS bioanalytical method development. The three themes that have recently emerged as central to building quality during method development-phospholipids, incurred sample and sound chromatographic considerations-are the main focus of this article. In order to reduce the bioanalytical risk associated with plasma phospholipids, a dual approach involving extraction and chromatography is recommended. The use of incurred sample during method development is essential to avoid interference arising from drug-related components. This is different from the current practice of incurred sample reanalysis, which tests reproducibility during method application. LC column/mobile phase optimization is needed to achieve appropriate peak shape, sensitivity and the separation of the analyte from interfering metabolites and phospholipids. Related to sound chromatographic considerations, we lay out facts and myths related to UPLC, vis-à-vis HPLC. Incorporation of quality during method development avoids the costly experience of finding out by chance about the invalidity of a method after it has been used in support of a number of pivotal clinical and non-clinical studies. To this end, we put forth an outline of a protocol for a systematic LC-MS/MS bioanalytical method development.

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“…Due to the reduced diffusion path for analytes, superficially porous particles are expected to have superior mass transfer properties compared with the fully porous particles and therefore provide similar separation efficiencies compared to sub-2 lm particles but at much lower pressures [12]. However, Gritti et al pointed out that the reduced diffusion path length is not the sole factor for high column efficiency.…”

Section: Introductionmentioning

confidence: 97%

Monolithic Silica for Fast HPLC: Current Success and Promising Future

Deeb

2011

Chromatographia

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As an approach for fast HPLC, monolithic silica has proven to be highly effective. It is especially successful for routinely obtaining fast isocratic HPLC analyses of small drug molecules. The low cost of monolithic compared with other approaches, such as UPLC, makes it more convenient for everyday application. It is also the more developed and widely applied technique compared with superficially porous particles. It offers the possibility for gaining high plate numbers through column coupling, but not at the expense of run time if a proper flow program is subsequently applied. Good precision and batch reproducibility are now achieved with commercially available monolithic silica columns. The application of monolithic silica columns is already well developed in various fields. It invades the field of bio-analysis and proteomics. Hundreds of analytical methods have already been successfully transferred to or developed on monolithic silica columns. An updated strategy based on Snyder's method for rapid method development using monolithic column has been provided. However, more is still to be expected from monolithic silica in term of chemistry variation, application, and instrument compatibility. The future of monolithic silica is promising when considering the high demand for fast chromatographic analysis.

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Critical comparison of performances of superficially porous particles and sub-2μm particles under optimized ultra-high pressure conditions

Cited by 83 publications

References 33 publications

Comparison of the kinetic performance and retentivity of sub-2μm core–shell, hybrid and conventional bare silica phases in hydrophilic interaction chromatography

Comparison of the kinetic performance and retentivity of sub-2μm core–shell, hybrid and conventional bare silica phases in hydrophilic interaction chromatography

Systematic LC‐MS/MS bioanalytical method development that incorporates plasma phospholipids risk avoidance, usage of incurred sample and well thought‐out chromatography

Monolithic Silica for Fast HPLC: Current Success and Promising Future

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