“…Surprisingly, only three previous studies have reported positive results with period lengthening to translation inhibitors: anisomycin lengthened the period to 31 hr or longer in Aplysia (28), and cycloheximide lengthened period up to 36 hr in Euglena (29) and up to 33 hr in Chlamydomonas (30). Possibly, expression of circadian rhythmicity is not 4 observable with constant inhibitor treatment at higher concentrations where period lengthening occurs (16,38,55 (35,43), Neurospora (36,40,43), Gonyaulax (38,42,(45)(46)(47), Phaseolus (39), Aplysia (31,34), and hamster (33,41) and for anisomycin pulses in Gonyaulax (37,38,42,45,47), Aplysia (27,28,32,34), chick pineal (49), and hamster (33,48). On the basis of these data, it has been suggested that translation is a requirement for the circadian pacemaker mechanism (24, 27, 28, 30-32, 35, 36, 40, 43, 44, 47, 49, 50), or more specifically, that translation is a phasedependent requirement (24,28,32,35,36,40,…”