Critical Review: digital resolution biomolecular sensing for diagnostics and life science research

Huang, Qinglan; Li, Nantao; Zhang, Hanyuan; Che, Congnyu; Sun, Fu; Xiong, Yanyu; Canady, Taylor D.; Cunningham, Brian T.

doi:10.1039/d0lc00506a

Cited by 42 publications

(26 citation statements)

References 288 publications

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“…In conclusion, nanophotonic biosensors have made substantial progress and demonstrated sufficient sensitivity for the observation of even individual molecular binding events 147,148 . However, translating these exciting scientific advances into biosensor devices for everyday life requires further efforts on multiple fronts.…”

Section: Future Perspectives and Challengesmentioning

confidence: 98%

Advances and applications of nanophotonic biosensors

et al. 2022

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Section: Future Perspectives and Challengesmentioning

confidence: 98%

Advances and applications of nanophotonic biosensors

et al. 2022

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“…For a recent review of digital-resolution biomolecular detection technologies, we refer the reader to Refs. [ 34 ].…”

Section: Introductionmentioning

confidence: 99%

Single-step, wash-free digital immunoassay for rapid quantitative analysis of serological antibody against SARS-CoV-2 by photonic resonator absorption microscopy

Zhao

Che

Wang

et al. 2021

Talanta

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“…These limitations can however be circumvented by employing large arrays of independently addressable transducers . Consistent with recent usage in both SEE and single-molecule optical measurements, − we employ the term “digital sensing” to refer to this combination of single-entity, real-time, and parallelized detection. This terminology evokes the high degree of integration characteristic of modern electronics.…”

Section: Introductionmentioning

confidence: 99%

Single-Entity Electrochemistry for Digital Biosensing at Ultralow Concentrations

Lemay

Moazzenzade

2021

Anal. Chem.

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Quantifying ultralow analyte concentrations is a continuing challenge in the analytical sciences in general and in electrochemistry in particular. Typical hurdles for affinity sensors at low concentrations include achieving sufficiently efficient mass transport of the analyte, dealing with slow reaction kinetics, and detecting a small transducer signal against a background signal that itself fluctuates slowly in time. Recent decades have seen the advent of methods capable of detecting single analytes ranging from the nanoscale to individual molecules, representing the ultimate mass sensitivity to these analytes. However, single-entity detection does not automatically translate into a superior concentration sensitivity. This is largely because electrochemical transducers capable of such detection are themselves miniaturized, exacerbating mass transport and binding kinetic limitations. In this Perspective, we discuss how these challenges can be tackled through so-called digital sensing: large arrays of separately addressable single-entity detectors that provide real-time information on individual binding events. We discuss the advantages of this approach and the barriers to its implementation.

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Critical Review: digital resolution biomolecular sensing for diagnostics and life science research

Abstract: One of the frontiers in the field of biosensors is the ability to quantify specific target molecules with enough precision to count individual units in a test sample, and to...

Cited by 42 publications

References 288 publications

Advances and applications of nanophotonic biosensors

Advances and applications of nanophotonic biosensors

Single-step, wash-free digital immunoassay for rapid quantitative analysis of serological antibody against SARS-CoV-2 by photonic resonator absorption microscopy

Single-Entity Electrochemistry for Digital Biosensing at Ultralow Concentrations

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