Critical roles of mecamylamine-sensitive mushroom body neurons in insect olfactory learning

Kuramochi

et al. 2013

Neuroscience Letters

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The molecular and neural basis of protein synthesis-dependent long-term memory (LTM) has been the subject of extensive studies in vertebrates and invertebrates. In crickets and honey bees, it has been demonstrated that nitric oxide (NO) signaling plays critical roles in LTM formation, but no experimental system appropriate for electrophysiological study of neural mechanisms by which production of NO leads to LTM formation has been established in insects. We have reported that cockroaches, as do dogs and humans, exhibit conditioning of salivation, i.e., they exhibit an increased level of salivation in response to an odor paired with sucrose reward. Salivary conditioning can be monitored by activity changes of salivary neurons in rigidly immobilized animals and thus is useful for the study of brain mechanisms of learning and memory. In the present study, we found that injection of cycloheximide, a protein synthesis inhibitor, into the hemolymph before multiple conditioning trials impairs formation of 1-day memory, but not that of 30-min memory. This indicates that formation of 1-day memory requires protein synthesis but that of earlier memory does not. We also found that injection of L-NAME, an inhibitor of NO synthase, before multiple conditioning impairs formation of 1-day memory but not that of 30-min memory. We thus conclude that NO signaling participates in the formation of protein synthesis-dependent LTM but not that of earlier memory in salivary conditioning. Salivary conditioning in cockroaches should become a pertinent system for the study of neural mechanisms by which activation of NO synthase leads to LTM formation.(250 words) ３

Section: Discussionmentioning

confidence: 77%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Participation of NO signaling in formation of long-term memory in salivary conditioning of the cockroach

Kuramochi

et al. 2013

Neuroscience Letters

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Arch Insect Biochem Physiol

CHARACTERIZATION OF NICOTINE ACETYLCHOLINE RECEPTOR SUBUNITS IN THE COCKROACH Periplaneta americana MUSHROOM BODIES REVEALS A STRONG EXPRESSION OF β1 SUBUNIT: INVOLVEMENT IN NICOTINE‐INDUCED CURRENTS

Taillebois

Thany

2016

Nicotinic acetylcholine receptors are ligand-gated ion channels expressed in many insect structures, such as mushroom bodies, in which they play a central role. We have recently demonstrated using electrophysiological recordings that different native nicotinic receptors are expressed in cockroach mushroom bodies Kenyon cells. In the present study, we demonstrated that eight genes coding for cockroach nicotinic acetylcholine receptor subunits are expressed in the mushroom bodies. Quantitative real-time polymerase chain reaction (PCR) experiments demonstrated that β1 subunit was the most expressed in the mushroom bodies. Moreover, antisense oligonucleotides performed against β1 subunit revealed that inhibition of β1 expression strongly decreases nicotine-induced currents amplitudes. Moreover, co-application with 0.5 μM α-bungarotoxin completely inhibited nicotine currents whereas 10 μM d-tubocurarine had a partial effect demonstrating that β1-containing neuronal nicotinic acetylcholine receptor subtypes could be sensitive to the nicotinic acetylcholine receptor antagonist α-bungarotoxin.

J of Comparative Neurology

Serotonin‐immunoreactive sensory neurons in the antenna of the cockroach Periplaneta americana

Watanabe

Shimohigashi

Yokohari

2013

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The antennae of insects contain a vast array of sensory neurons that process olfactory, gustatory, mechanosensory, hygrosensory, and thermosensory information. Except those with multimodal functions, most sensory neurons use acetylcholine as a neurotransmitter. Using immunohistochemistry combined with retrograde staining of antennal sensory neurons in the cockroach Periplaneta americana, we found serotonin-immunoreactive sensory neurons in the antenna. These were selectively distributed in chaetic and scolopidial sensilla and in the scape, the pedicel, and first 15 segments of the flagellum. In a chaetic sensillum, A single serotonin-immunoreactive sensory neuron cohabited with up to four serotonin-negative sensory neurons. Based on their morphological features, serotonin-immunopositive and -negative sensory neurons might process mechanosensory and contact chemosensory modalities, respectively. Scolopidial sensilla constitute the chordotonal and Johnston's organs within the pedicel and process antennal vibrations. Immunoelectron microscopy clearly revealed that serotonin-immunoreactivities selectively localize to a specific type of mechanosensory neuron, called type 1 sensory neuron. In a chordotonal scolopidial sensillum, a serotonin-immunoreactive type 1 neuron always paired with a serotonin-negative type 1 neuron. Conversely, serotonin-immunopositive and -negative type 1 neurons were randomly distributed in Johnston's organ. In the deutocerebrum, serotonin-immunoreactive sensory neuron axons formed three different sensory tracts and those from distinct types of sensilla terminated in distinct brain regions. Our findings indicate that a biogenic amine, serotonin, may act as a neurotransmitter in peripheral mechanosensory neurons.