Croatian Society of Medical Biochemistry and Laboratory Medicine: National recommendations for blood collection, processing, performance and reporting of results for coagulation screening assays prothrombin time, activated partial thromboplastin time

Bronić, Ana; Herak, Désirée Coen; Margetić, Sandra; Milić, Marija

doi:10.11613/bm.2019.020503

Cited by 27 publications

(13 citation statements)

References 53 publications

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“…Coagulation biomarkers (prothrombin time (PT), activated partial thromboplastin time (aPTT), fibrinogen (Fbg), D-dimers)) were measured on BCS XP coagulation analyser (Siemens, Marburg, Germany), by the following methods: PT – photo-optical method (Dade Innovin %), APTT – photo-optical method (Dade Actin FS), Fbg – modification of the Clauss method (Multifibren U), and D-dimers – particle-enhanced immunoturbidimetric assay (Innovance D-dimer)). An aPTT-ratio was calculated according to the National recommendations for blood collection, processing, performance and reporting of results for coagulation screening assays prothrombin time, activated partial thromboplastin time, thrombin time, fibrinogen and D-dimer ( 19 ). The recommendations state that the aPTT ratio is calculated as the ratio of patient’s aPTT divided by the mean value of the reference interval for the particular system (reagent/coagulometer) used.…”

Section: Methodsmentioning

confidence: 99%

Women in sports: the applicability of common national reference intervals for inflammatory and coagulation biomarkers (HemSter Study)

Biljak

Vidranski²,

Ružić

et al. 2021

Biochem. med. (Online)

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Introduction Intensive physical activity causes functional and metabolic changes in the athlete’s organism. The study aimed to verify the common national available reference intervals (RIs) for common inflammatory and screening coagulation tests in a population of healthy young female athletes. Materials and methods One hundred and twenty-one female athletes (age range: 16–34), from various sports disciplines (water polo, handball, volleyball, football, basketball), were included in the study. All participants completed the international physical activity short-form questionnaire. Blood samples were collected between 8–10 am, after an overnight fast, before any physical activity. Reference intervals were determined according to Clinical & Laboratory Standards Institute EP28-A3C Guidelines. Results Calculated RIs for white blood cell count (WBC), prothrombin time (PT), and activated partial thromboplastin time (APTT) ratio were in accordance with the common national RIs. Calculated RI for C-reactive protein (CRP) was lower (< 2.9 mg/L) than the proposed cut-off for a healthy population (< 5.0 mg/L). Reference interval for fibrinogen was higher (1.9–4.4 g/L), than the available RIs (1.8–3.5 g/L). D-dimer cut-off value was set at 852 µg/L fibrinogen equivalent units (FEU), higher than the proposed 500 µg/L FEU for venous thromboembolism (VTE) exclusion. Conclusions The applicability of the available RIs for WBC count, PT, and APTT-ratio was confirmed. However, RIs for CRP and fibrinogen differed significantly than the available common national RIs for the healthy non-athletes’ population. A higher cut-off for D-dimers should be extensively verified before implementation for VTE diagnosis exclusion in a group of healthy young female athletes.

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Section: Methodsmentioning

confidence: 99%

Women in sports: the applicability of common national reference intervals for inflammatory and coagulation biomarkers (HemSter Study)

Biljak

Vidranski²,

Ružić

et al. 2021

Biochem. med. (Online)

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“…CBC was performed within half an hour from blood sampling. Tubes with sodium citrate were centrifuged two times at 1500 x g for 15 min, as per national recommendations [33], and serum tubes with gel were centrifuged at 2000 g for 10 min, as recommended by the manufacturer. WBC counts were performed on a Sysmex XN-1000 analyzer (Sysmex Corporation, Kobe, Japan) as part of the CBC.…”

Section: Measurement Of Hematological and Biochemical Parametersmentioning

confidence: 99%

Uric acid and uric acid to creatinine ratio in the assessment of chronic obstructive pulmonary disease: Potential biomarkers in multicomponent models comprising IL-1beta

et al. 2020

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Chronic obstructive pulmonary disease (COPD) is a complex and heterogeneous disease, with oxidative stress and inflammation implicated in its development. Uric acid (UA) could exert anti-oxidative, pro-oxidative or pro-inflammatory effects, depending on the specific context. It was recently shown that soluble UA, and not just its crystals, could activate the nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome, leading to interleukin (IL)-1β secretion. We aimed to assess the differences in blood levels of UA and its ratio with creatinine (UCR) between COPD patients and healthy subjects, as well as their association with disease severity, smoking status, common COPD comorbidities and therapy regimes. The diagnostic characteristics of UA and UCR were also explored. This study included 109 stable COPD patients and 95 controls and measured white blood cells (WBC), C-reactive protein (CRP), fibrinogen (Fbg), IL-1β, creatinine (CREAT) and UA. All of the parameters were increased in COPD patients, except for CREAT. UA and UCR were positively associated with WBC, CRP and IL-1β. COPD smokers had lower UA and UCR values. Common COPD therapy did not affect UA or UCR, while patients with cardiovascular diseases (CVD) had higher UA, but not UCR, levels. Patients with higher UCR values showed worse disease-related outcomes (lung function, symptoms, quality of life, history of exacerbations, BODCAT and BODEx). Also, UCR differentiated patients with different severity of airflow limitation as well as symptoms and exacerbations. The great individual predictive potential of UCR and IL-1β was observed with their odds ratios (OR) being 2.09 and 5.53, respectively. Multiparameter models of UA and UCR that included IL-1β were able to correctly classify 86% and 90% of cases, respectively. We suggest that UA might be a useful biomarker when combined with IL-1β, while UCR might be even more informative and useful in overall COPD assessments.

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“…If samples were stored frozen for a certain period of time, the thawing procedure may affect sample integrity, at least for some analytes (e.g. coagulation parameters) [17]. For this reason, if samples were stored frozen, authors should provide the detailed description about how samples were thawed and the number of freeze thaw cycles.…”

Section: Preanalytical Conditions (Conditions Prior To the Basal Timementioning

confidence: 99%

The CRESS checklist for reporting stability studies: on behalf of the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) Working Group for the Preanalytical Phase (WG-PRE)

Cornes

Šimundić

Cadamuro

et al. 2020

Clinical Chemistry and Laboratory Medicine (CCLM)

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To ensure that clinical laboratories produce results that are both accurate and of clinical utility it is essential that only samples of adequate quality are analysed. Although various studies and databases assessing the stability of analytes in different settings do exist, guidance on how to perform and report stability studies is lacking. This results in studies that often do not report essential information, thus compromising transferability of the data. The aim of this manuscript is to describe the Checklist for Reporting Stability Studies (CRESS) against which future studies should be reported to ensure standardisation of reporting and easy assessment of transferability of studies to other healthcare settings. The EFLM WG-PRE (European Federation of Clinical Chemistry and Laboratory Medicine Working Group for the Preanalytical Phase) produced the CRESS checklist following a detailed literature review and extensive discussions resulting in consensus agreement. The checklist consists of 20 items covering all the aspects that should be considered when producing a report on a stability study including details of what should be included for each item and a rationale as to why. Adherence to the CRESS checklist will ensure that studies are reported in a transparent and replicable way. This will allow other laboratories to assess whether published data meet the stability criteria required in their own particular healthcare scenario. The EFLM WG-PRE encourage researchers and authors to use the CRESS checklist as a guide to planning stability studies and to produce standardised reporting of future stability studies.

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Croatian Society of Medical Biochemistry and Laboratory Medicine: National recommendations for blood collection, processing, performance and reporting of results for coagulation screening assays prothrombin time, activated partial thromboplastin time

Cited by 27 publications

References 53 publications

Women in sports: the applicability of common national reference intervals for inflammatory and coagulation biomarkers (HemSter Study)

Women in sports: the applicability of common national reference intervals for inflammatory and coagulation biomarkers (HemSter Study)

Uric acid and uric acid to creatinine ratio in the assessment of chronic obstructive pulmonary disease: Potential biomarkers in multicomponent models comprising IL-1beta

The CRESS checklist for reporting stability studies: on behalf of the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) Working Group for the Preanalytical Phase (WG-PRE)

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