Croceivirga radicis gen. nov., sp. nov., isolated from a rotten tropical mangrove root

Abstract

A novel bacterial strain, designated HSG9, was isolated from aisolated from a rotten tropical mangrove root. Cells of strain HSG9 were aerobic, Gram-stain-negative, yellow, oxidase-negative and catalase-positive. Growth was observed in 0.5-9 % sea salt (optimum 3 %, w/v), at 10-42 °C (optimum 25-35 °C) and at pH 6.0-8.0 (optimum 7.0-8.0). Gelatin, esterase and Tweens 20, 40, 60 and 80 were hydrolysed, but starch, protein, cellulose and casein were not. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain HSG9 formed an independent lineage related to the family Flavobacteriaceae. The dominant fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and iso-C15 : 1 G. The respiratory quinone was identified as MK-6 and the polar lipids were phosphatidylethanolamine, three unidentified phospholipids and an unidentified lipid. The DNA G+C content was 37.1 mol%. The combined genotypic and phenotypic data indicated that strain HSG9 represents a novel species of a new genus within the family Flavobacteriaceae, for which the name Croceivirgaradicis gen. nov., sp. nov. is proposed. The type strain is HSG9 (=MCCC 1A06690=KCTC 52589).

Analysis of 1,000 Type-Strain Genomes Improves Taxonomic Classification of Bacteroidetes

“…This solution appears to be preferable compared to a recent proposal that only transferred Spongiibacterium to Flagellimonas (Choi et al, 2018). Additional supermatrix analyses indeed confirmed that Flagellimonas and Spongiibacterium are nested within Muricauda , whereas Croceitalea (Lee et al, 2008; Yoon and Oh, 2012; Su et al, 2017) and Croceivirga (Hu et al, 2017) form independent lineages (Supplementary Data Sheet 1). Whereas, the SH tests indicated a significant conflict between the best supermatrix and best 16S rRNA gene ML trees, the latter also showed Spongiibacterium and Flagellimonas nested within Muricauda (Supplementary Table 1).…”

Croceivirga litoralis sp. nov., isolated from coastal surface water, and reclassification of Muricauda lutea as Croceivirga lutea comb. nov.

“…in 2017 [1], and belongs to the family Flavobacteriaceae [2], which is an important lineage of the phylum Bacteroidetes [3]. At the time of writing, the genus Croceivirga comprises only one species, Croceivirga radices , which was isolated from a rotten tropical mangrove root [1]. Generally, members of the genus Croceivirga are Gram-stain-negative, rod-shaped and chemoheterotrophic.…”

“…Cell growth requires NaCl and oxygen. The respiratory quinone is menaquinone with six isoprene units (MK-6) [1]. In this study, a polyphasic taxonomic approach was used to determine the taxonomic position of strain ASW18 T , considered to represent a novel species of the genus Croceivirga .…”

“…iso-C 15 : 0 (33.8 %), iso-C 17 : 0 3-OH (27.8 %) and iso-C 15 : 1 G (10.7 %) were the major Table 1. Differential characteristics between strain ASW18 T and the reference strains Strains: 1, ASW18 T ; 2, Croceivirga radices MCCC 1A06690 T [1]; 3, Muricauda lutea MCCC 1K03195 T [5]. The results from tests with API strips (bioMérieux), GNⅢ MicroPlates (Biolog) and for polar lipids of the three strains are from this study; other data for the reference strains are taken from the original publications [1,5].…”

Paramesonia marina gen. nov., sp. nov., isolated from deep-sea water of the Indian Ocean

“…At the time of writing, the family Flavobacteriaceae comprises more than 150 genera. Flavobacteriaceae is widespread in the aquatic environment and especially in marine environments including seawater, marine sediment, marine plants, marine animals and so on [5][6][7][8][9][10]. The species of this family are generally Gram-stain-negative, rod-shaped, chemoheterotrophic and contain menaquinone 6 (MK-6) as the sole respiratory quinone [11,12].…”