Crop Breeding for Sustainable Agriculture, Genomics Interventions in

Kulwal, Pawan L.; Thudi, Mahendar; Varshney, Rajeev K.

doi:10.1007/978-1-4614-5797-8_271

Cited by 3 publications

(1 citation statement)

References 62 publications

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“…This understanding has made it possible to employ techniques such as marker-assisted selection (MAS), QTL analysis, and AM studies, or to directly compute and employ genomic selection (GS) to produce high-value genotypes for breeding programs. (Kulwal et al, 2011).…”

Section: Genomic Assisted Breeding For Developing Climate Resilient C...mentioning

confidence: 99%

A review on conventional and modern breeding approaches for developing climate resilient crop varieties

Prabhu,

Kumar,

Yumkhaibam

et al. 2023

JANS

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The escalating threat of climate change is a major challenge to global food security. One of the ways to mitigate its impact is by developing crops that can withstand environmental stresses such as drought, heat, and salinity. Plant breeders have been employing conventional and modern approaches to achieve climate-resilient crops. Climate-resilient crops refer to both crop and crop varieties that exhibit improved tolerance towards biotic and abiotic stresses. These crops possess the capacity to maintain or even increase their yields when exposed to various stress conditions, such as drought, flood, heat, chilling, freezing and salinity. Conventional breeding entails selecting and crossing plants with desirable traits, while modern breeding deploys molecular techniques to identify and transfer specific genes associated with stress tolerance. However, the effectiveness of both methods is contingent on the crop species and the targeted stress. Advancements in gene editing, such as CRISPER-cas9 and genomics-assisted breeding, offer new opportunities to hasten the development of climate-resilient crops. These new technologies include Marker Assisted Selection, Genome-Wide Association Studies, Mutation breeding, Transcriptomics, Genomics, and more. The review concludes that these cutting-edge techniques have the potential to enhance the speed and precision of developing crops that can endure the challenges posed by climate change.

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Section: Genomic Assisted Breeding For Developing Climate Resilient C...mentioning

confidence: 99%

A review on conventional and modern breeding approaches for developing climate resilient crop varieties

Prabhu,

Kumar,

Yumkhaibam

et al. 2023

JANS

View full text Add to dashboard Cite

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Quantitative Trait Locus Mapping of Clubroot Resistance and Plasmodiophora brassicae Pathotype Banglim-Specific Marker Development in Brassica rapa

Choi

Chhapekar

et al. 2020

IJMS

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Clubroot resistance is an economically important trait in Brassicaceae crops. Although many quantitative trait loci (QTLs) for clubroot resistance have been identified in Brassica, disease-related damage continues to occur owing to differences in host variety and constant pathogen variation. Here, we investigated the inheritance of clubroot resistance in a double haploid population developed by crossing clubroot resistant and susceptible lines “09CR500” and “09CR501”, respectively. The resistance of “09CR500” to Plasmodiophora brassicae pathotype “Banglim” was controlled as a single dominant gene, with the segregation of resistance and susceptibility being nearly 1:1. PbBrA08Banglim was identified as having a logarithm of odds value of 7.9–74.8, and a phenotypic variance of 26.0–97.1% with flanking marker “09CR.11390652” in A08. After aligning QTL regions to the B. rapa reference genome, 11 genes were selected as candidates. PbBrA08Banglim was located near Crr1, CRs, and Rcr9 loci, but differences were validated by marker analysis, gene structural variations, and gene expression levels, as well as phenotypic responses to the pathotype. Genotyping using the “09CR.11390652” marker accurately distinguished the Banglim-resistance phenotypes in the double haploid population. Thus, the developed marker will be useful in Brassica breeding programs, marker-assisted selection, and gene pyramiding to identify and develop resistant cultivars.

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MiR1885 Regulates Disease Tolerance Genes in Brassica rapa during Early Infection with Plasmodiophora brassicae

Pavlidis

Chhapekar

Rameneni

et al. 2021

IJMS

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Clubroot caused by Plasmodiophora brassicae is a severe disease of cruciferous crops that decreases crop quality and productivity. Several clubroot resistance-related quantitative trait loci and candidate genes have been identified. However, the underlying regulatory mechanism, the interrelationships among genes, and how genes are regulated remain unexplored. MicroRNAs (miRNAs) are attracting attention as regulators of gene expression, including during biotic stress responses. The main objective of this study was to understand how miRNAs regulate clubroot resistance-related genes in P. brassicae-infected Brassica rapa. Two Brassica miRNAs, Bra-miR1885a and Bra-miR1885b, were revealed to target TIR-NBS genes. In non-infected plants, both miRNAs were expressed at low levels to maintain the balance between plant development and basal immunity. However, their expression levels increased in P. brassicae-infected plants. Both miRNAs down-regulated the expression of the TIR-NBS genes Bra019412 and Bra019410, which are located at a clubroot resistance-related quantitative trait locus. The Bra-miR1885-mediated down-regulation of both genes was detected for up to 15 days post-inoculation in the clubroot-resistant line CR Shinki and in the clubroot-susceptible line 94SK. A qRT-PCR analysis revealed Bra019412 expression was negatively regulated by miR1885. Both Bra019412 and Bra019410 were more highly expressed in CR Shinki than in 94SK; the same expression pattern was detected in multiple clubroot-resistant and clubroot-susceptible inbred lines. A 5′ rapid amplification of cDNA ends analysis confirmed the cleavage of Bra019412 by Bra-miR1885b. Thus, miR1885s potentially regulate TIR-NBS gene expression during P. brassicae infections of B. rapa.

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Crop Breeding for Sustainable Agriculture, Genomics Interventions in

Cited by 3 publications

References 62 publications

A review on conventional and modern breeding approaches for developing climate resilient crop varieties

A review on conventional and modern breeding approaches for developing climate resilient crop varieties

Quantitative Trait Locus Mapping of Clubroot Resistance and Plasmodiophora brassicae Pathotype Banglim-Specific Marker Development in Brassica rapa

MiR1885 Regulates Disease Tolerance Genes in Brassica rapa during Early Infection with Plasmodiophora brassicae

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