Cross-correlation capillary electrophoresis in unmodified commercial equipment

Seiman, Andrus

doi:10.1016/j.proche.2009.12.011

Cited by 4 publications

(4 citation statements)

References 25 publications

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“…System Setup and Optimization. Seiman and coworkers 65 studied in their cross-correlation approach a system at a relatively high pH of 8.46 with a naturally strong EOF that they argued was subject to drifting. Resulting temporal errors in HT were deemed responsible for unsuccessful deconvolution outcomes.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…That is because they rely on additional custom-made injectors in order to perform alternating sample and blank injections without having to disconnect the system from continuous voltage. Such hardware typically comprises pneumatic autosamplers, , micromachined inlet ports, or switches of various shapes ,,, and setups for optical gating. , To our knowledge, there has yet only been one research report by Seiman and co-workers that examined cross-correlation CE without additional hardware and investigated effects of repeated voltage suspensions. They realized 6-bit PRBS and SNR improvements of 2.8 (4.0 theoretical maximum), however, only with a single analyte present.…”

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confidence: 99%

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Direct Hadamard Transform Capillary Zone Electrophoresis without Instrumental Modifications

et al. 2018

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We report the first successful implementation of a multiplexing method on a standard capillary electrophoresis system with UV detection that is independent of additional hardware. This was achieved using the Hadamard transform approach and employing vial exchange and voltage suspensions for translation of pseudorandom binary sequence elements into sample and background electrolyte injections of a capillary zone electrophoresis separation. Sequences exceeding peak capacity of the capillary were subdivided into shorter subsequences measured successively and realigned afterward based on EOF marker or analyte peaks. This way, we realized and deconvoluted modulation sequences as long as 8-bit (255 injections) for two systems containing either AMP or a mixture of the nucleotides (A,C,G,U)MP resulting in electropherograms of considerably improved signal-to-noise ratio. We achieved factors of intensity enhancement of around 6.9 and 5.2, respectively (theoretical maximum 8.0). This contribution, further, presents experimental and simulation studies on the effects on zones during injection and separation when experiencing voltage suspensions. Besides analysis of EOF behavior and influence of diffusion dispersion, we also provide data on the significance of specific electrophoretic errors such as peak position shift, inconsistent sample injection, and peak broadening on the quality of the inverse Hadamard transform. Moreover, the application of our approach to the practical analysis of a milk sample is described. The results demonstrate the applicability of multiplexing on unmodified standard CE instrumentation and establish a new suitable methodology to enhance the low sensitivity of on-column UV detection in capillary electrophoresis.

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Section: ■ Results and Discussionmentioning

confidence: 99%

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confidence: 99%

Direct Hadamard Transform Capillary Zone Electrophoresis without Instrumental Modifications

et al. 2018

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“…The same group also proposed specific ways of programming the instrument (Beckmann P/ACE 5500) and performing a sequential electrokinetic injection [245]. Using an EOF marker as a sample to find optimal conditions for cross-correlation experiments, S/N ratio improvements of around 2.8 (compared to 4.0 theoretical) were obtained.…”

Section: - Innovative Uses and Modifications Of Classic Ce Instrumenmentioning

confidence: 99%

Recent developments in instrumentation for capillary electrophoresis and microchip‐capillary electrophoresis

2010

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Over the last years there has been an explosion in the number of developments and applications of capillary electrophoresis (CE) and microchip-CE. In part, this growth has been the direct consequence of recent developments in instrumentation associated with CE. This review, which is focused on contributions published in the last five years, is intended to complement the papers presented in this special issue dedicated to Instrumentation and to provide an overview on the general trend and some of the most remarkable developments published in the areas of high voltage power supplies, detectors, auxiliary components, and compact systems. It also includes few examples of alternative uses of and modifications to traditional CE instruments.

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“…cross-correlation CE [4][5][6] or Hadamard transform CE [7,8], both of which measure several signals simultaneously, yielding an electropherogram with improved signal-to-noise).…”

Section: Introductionmentioning

confidence: 99%

Thermal marks as a signal processing aid for a portable capillary electropherograph

2011

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The interpretation of raw signals in capillary CE can be challenging if there are unknown peaks, or the signal is corrupt due to baseline fluctuations, EOF velocity drift, etc. Signal processing could be required before results can be interpreted. A suite of signal processing algorithms has been developed for CE data analysis, specifically for use in field experiments for the detection of nerve agents using portable CE instruments. Everything from baseline correction and electropherogram alignment to peak matching and identification is included in these programs. Baseline correction is achieved by interpolating a new baseline according to points found using all local extremes, by applying an appropriate outliers test. Irreproducible migration times are corrected by compensating for EOF drift, measured with the aid of thermal marks. Thermal marks are small disturbances in the capillary created by punctual heating that move with the velocity of EOF. Peaks in the sample electropherogram are identified using a fuzzy matching algorithm, by comparing peaks from the sample electropherogram to peaks from a reference electropherogram.

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Cross-correlation capillary electrophoresis in unmodified commercial equipment

Cited by 4 publications

References 25 publications

Direct Hadamard Transform Capillary Zone Electrophoresis without Instrumental Modifications

Direct Hadamard Transform Capillary Zone Electrophoresis without Instrumental Modifications

Recent developments in instrumentation for capillary electrophoresis and microchip‐capillary electrophoresis

Thermal marks as a signal processing aid for a portable capillary electropherograph

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