Cross-Correlation Techniques in Chromatography

Annino, Raymond; Grushka, Eli

doi:10.1093/chromsci/14.6.265

Cited by 34 publications

(14 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Certain pseudorandom sequences can be found whose auto-correlations are delta functions at zero delay time (17). For these sequences Equation 7simplifies to: (8) If a member of this special group of input signals is used, the impulse response function (chromatogram) can be found by computing the cross-correlation of the input and output signals.…”

Section: Definition Of a Chromatographicmentioning

confidence: 99%

Multiplex gas chromatography

Phillips¹

1980

Anal. Chem.

View full text Add to dashboard Cite

Output signals of multiplex techniques are not directly interpretable by a human operator, but their greater information content can be recovered with a computerMultiplex gas chromatography is an alternative to the batch separation mode employed in the vast majority of gas chromatographic analytical procedures. Either produces the same information (i.e., the chromatogram), but for certain applications the multiplex mode offers some very important advantages.

show abstract

Section: Definition Of a Chromatographicmentioning

confidence: 99%

Multiplex gas chromatography

Phillips¹

1980

Anal. Chem.

View full text Add to dashboard Cite

show abstract

“…The multiplex advantage of cross-correlation chro-matography has been assumed with out actually mentioning the term by a number of people (7)(8)(9)(15)(16)(17) and has been specifically pointed out for the closely related Hadamard transform chromatography (19). The en coded information can be recovered through a number of data processing techniques, some of which are de scribed in this paper.…”

Section: The Multiplex and Throughput Advantagesmentioning

confidence: 99%

“…Certain pseudorandom sequences can be found whose auto-correlations are delta functions at zero delay time (17). For these sequences Equation 7simplifies to: h(r) = FT"1 FT(Output ® Input) 1 = Output ® Input (8) If a member of this special group of input signals is used, the impulse response function (chromatogram) can be found by computing the cross-correlation of the input and output signals.…”

Section: Definition Of a Chromatographic Systemmentioning

confidence: 99%

Report

1980

Anal. Chem.

View full text Add to dashboard Cite

show abstract

“…gas chromatography (GC), − LC, − ion mobility, , and mass spectrometry (MS). − In separation applications, the modulation of the input information–the analyte signal–is achieved by means of pseudorandom binary sequences (PRBS) that consist of defined consecutions of “1” and “0” coding for the injection of sample and blank, respectively. − Their number of elements m is denoted by the exponent n (-bit) according to m = 2 n – 1. Cross-correlation of the overlapped detector signal with the input sequence allows, subsequently, for the deconvolution of the conventional chromatographic information resulting in an increased SNR. − The principles of Hadamard transform and cross-correlation techniques have been described in detail in the literature. ,,,, …”

mentioning

confidence: 99%

“…42−46 The principles of Hadamard transform and cross-correlation techniques have been described in detail in the literature. 15,16,18,19,42 The HT approach has been realized in modes of CE such as capillary zone electrophoresis (CZE), 47,48 micellar electrokinetic chromatography (MEKC), 49 and microchip CE 50−53 using a variety of different detectors such as UV, 54−57 LIF, 52,58,59 and amperometry. 53 Imasaka was the first to utilize HT-CZE in combination with fluorescence detection to expand the LOD of analytes into the subnano-and even subpicomolar scale.…”

mentioning

confidence: 99%

Direct Hadamard Transform Capillary Zone Electrophoresis without Instrumental Modifications

et al. 2018

View full text Add to dashboard Cite

We report the first successful implementation of a multiplexing method on a standard capillary electrophoresis system with UV detection that is independent of additional hardware. This was achieved using the Hadamard transform approach and employing vial exchange and voltage suspensions for translation of pseudorandom binary sequence elements into sample and background electrolyte injections of a capillary zone electrophoresis separation. Sequences exceeding peak capacity of the capillary were subdivided into shorter subsequences measured successively and realigned afterward based on EOF marker or analyte peaks. This way, we realized and deconvoluted modulation sequences as long as 8-bit (255 injections) for two systems containing either AMP or a mixture of the nucleotides (A,C,G,U)MP resulting in electropherograms of considerably improved signal-to-noise ratio. We achieved factors of intensity enhancement of around 6.9 and 5.2, respectively (theoretical maximum 8.0). This contribution, further, presents experimental and simulation studies on the effects on zones during injection and separation when experiencing voltage suspensions. Besides analysis of EOF behavior and influence of diffusion dispersion, we also provide data on the significance of specific electrophoretic errors such as peak position shift, inconsistent sample injection, and peak broadening on the quality of the inverse Hadamard transform. Moreover, the application of our approach to the practical analysis of a milk sample is described. The results demonstrate the applicability of multiplexing on unmodified standard CE instrumentation and establish a new suitable methodology to enhance the low sensitivity of on-column UV detection in capillary electrophoresis.

show abstract

Cross-Correlation Techniques in Chromatography

Cited by 34 publications

References 0 publications

Multiplex gas chromatography

Multiplex gas chromatography

Report

Direct Hadamard Transform Capillary Zone Electrophoresis without Instrumental Modifications

Contact Info

Product

Resources

About