Cross-genera amplification of Cajanus spp. specific SSR markers in Clitoria ternatea (L.) and their application in genetic diversity studies

Naik, Aparupa; Mishra, Satyasis; Nag, Atul; Soren, Gopal K; Panda, Aditya K.; Panda, Sanjib Kumar; Panigrahi, Jogeswar

doi:10.1007/s12298-020-00907-x

Cited by 2 publications

(1 citation statement)

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“…Although markers such as RAPD and ISSR have been utilized, the comprehensive documentation of DNA marker selection for butterfly pea genetic diversity analysis is lacking [9,10,13]. A recent study on genetic diversity among 14 genotypes of Cajanus spp., in India, showed a moderate variation of 55.1% using SSR primers [27].…”

Section: Discussionmentioning

confidence: 99%

The First Report on the Application of ISSR Markers in Genetic Variance Detection among Butterfly Pea (Clitoria ternatea L.) Accession in North Maluku Province, Indonesia

Nurhasanah,

Hindersah,

Suganda

et al. 2023

Horticulturae

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Butterfly pea (Clitoria ternatea L.) is a leguminous plant with several potential health benefits. The scientific name is derived from its origin on Ternate Island, North Maluku. Therefore, this study aimed to analyze the genetic variability in butterfly pea using Intergenic Simple Sequence Repeat (ISSR) markers in North Maluku. Field surveys, collection trips, and habitat studies of butterfly pea plants were conducted on Ternate, Tidore, Halmahera, and Morotai Islands. Genetic diversity was analyzed based on molecular data from the ISSR method. The molecular analysis results obtained using PCR-ISSR on 18 accessions showed a low degree of similarity. Among these, 15 accessions from Ternate, Tidore, Morotai, and Halmahera were in group A, while 3 from Ternate, Tidore, and Halmahera were in group B. All accessions exhibited a genetic similarity level of 0.709, indicating significant diversity. The arrangement among accessions on the dendrogram was similar to the phylogenetic tree, showing separation and spread at 0.608–0.924 based on the Jaccard coefficient. The results suggested that C. ternatea probably originated from Ternate, and subsequently spread to Tidore, Halmahera, and Morotai due to its use as a herbal medicine and ornamental plant. This information could be used as the basis for butterfly pea conservation and cultivation activities in Indonesia, specifically in Ternate Island, North Maluku.

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