2010
DOI: 10.1016/j.biortech.2010.03.084
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Cross-linked enzyme aggregates of recombinant Pseudomonas putida nitrilase for enantioselective nitrile hydrolysis

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Cited by 54 publications
(29 citation statements)
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“…Thermal stability of LI22-CLEAs can be important for chemical processes conducted at high temperatures. The CLEAs of other enzymes also showed thermal stability as compared to soluble enzyme [30][31][32]. Fig.…”
Section: Cross-linked Enzyme Aggregates (Cleas)mentioning
confidence: 92%
“…Thermal stability of LI22-CLEAs can be important for chemical processes conducted at high temperatures. The CLEAs of other enzymes also showed thermal stability as compared to soluble enzyme [30][31][32]. Fig.…”
Section: Cross-linked Enzyme Aggregates (Cleas)mentioning
confidence: 92%
“…The prevalent application of biocatalysis in non-aqueous 10 media has included stereo-selective synthesis of enantiopure 11 drugs over the past two decades [1][2][3][4]. The production of 12 enantiomerically pure compounds has gained significant impor- 13 tance in chemical and pharmaceutical industry.…”
mentioning
confidence: 99%
“…CLEA was formed by precipitation of enzyme and other proteins (impurities) coupled with cross-linking as described in literature (Kumar et al, 2010). 1 ml enzyme extract (2.16 mg protein, 1.3 U AMG activity) was mixed with appropriate amount of cross-linker to give desired final concentration and volume was made to 8 ml by 50 mM phosphate buffer (pH 7.0).…”
Section: Formation Of Cleamentioning
confidence: 99%