Cross-linked poly(vinyl alcohol) as permanent hydrophilic column coating for capillary electrophoresis

Belder, Detlev; Deege, Alfred; Husmann, H.; Köhler, Frank H.; Ludwig, Martin

doi:10.1002/1522-2683(200109)22:17<3813::aid-elps3813>3.0.co;2-d

Cited by 123 publications

(55 citation statements)

References 29 publications

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“…Currently we are working on a process to generate chemically cross-linked PVAcoatings in microfluidic devices which should exhibit an improved stability and improved robustness of the coating procedure as observed in classical capillaries [38].…”

Section: Resultsmentioning

confidence: 99%

“…Numerous hydrophilic capillary coatings have been developed in classical CE [28][29][30][31][32][33][34][35][36]. Among all the capillary coatings described in the literature those based on poly(vinyl alcohol) (PVA), which has to be considered the most hydrophilic nonionic polymer, have proven to exhibit a particularly good performance [37,38]. In CE separations using capil- laries coated with PVA, extraordinary high separation efficiencies could be achieved, especially for proteins.…”

Section: Introductionmentioning

confidence: 99%

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Poly(vinyl alcohol)-coated microfluidic devices for high-performance microchip electrophoresis

et al. 2002

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The channels of microfluidic glass chips have been coated with poly(vinyl alcohol) (PVA). Applied for microchip electrophoresis, the coated devices exhibited a suppressed electroosmotic flow and improved separation performance. The superior performance of PVA-coated channels could be demonstrated by electrophoretic separations of labeled amines and by video microscopy. While a distorted sample zone is injected using uncoated channels the application of PVA-coated channels results in an improved shape of the sample zone with less band broadening. Applying PVA-coated microchips for the separation of amines labeled with Alexa Fluor 350 even sub-second separations, utilizing a separation length of only 650 microm, could be obtained, while this was not possible using uncoated devices. By using PVA-coated devices rather than an uncoated chip a threefold increase in separation efficiencies could be observed. As the electroosmotic flow (EOF) was suppressed, the anionic compounds were detected at the anode whereas the dominant EOF in uncoated devices resulted in an effective mobility to the cathode. Besides improved separation performance another important feature of the PVA-coated channels was the suppressed adsorption of fluorescent compounds in repetitive runs which results in an improved robustness and detection sensitivity. Applying PVA-coated channels, rinsing or etching steps could be omitted while this was necessary for a reliable operation of uncoated devices.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Poly(vinyl alcohol)-coated microfluidic devices for high-performance microchip electrophoresis

et al. 2002

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“…One obvious possibility to improve the resolution in chiral microchip CE is to increase the length of the separation channel [27]. We are currently working on an approach to improve the resolution without changing the chip-layout by the use of coated micro channels in order to reduce analyte-wall interactions and the electroosmotic flow [28,29]. While for the FITC-labelled amines it was necessary to use blue light for excitation (450-480 nm) excitation, the green spectral region (515-560 nm) was required for the fluorescence detection of rhodamine derivatives.…”

Section: Resultsmentioning

confidence: 99%

Design and performance of a microchip electrophoresis instrument with sensitive variable-wavelength fluorescence detection

et al. 2002

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A modular instrument for high-speed microchip electrophoresis (MCE) equipped with a sensitive variable-wavelength fluorescence detection system was developed and evaluated. The experimental setup consists mainly of a lamp-based epifluorescence microscope for variable-wavelength fluorescence detection and imaging and a programmable four-channel bipolar high-voltage source capable of delivering up to +/- 10 kV per channel. The optical unit was equipped with a high-sensitivity photomultiplier tube and an adjustable aperture. The system was applied to MCE separations of flurescein isothiocyanate (FITC)-labelled amines utilizing blue light (450-480 nm) for excitation as well as for the separation of rhodamines utilizing excitation light in the green spectral region (531-560 nm). At optimized conditions baseline separation of four FITC-labelled amines could be obtained in less than 50 s at a detection limit of 460 ppt (1 nM) with a signal-to-noise ratio of 3:1. Three rhodamines could be baseline-separated in less than 6 s at a detection limit of 240 ppt (500 pM). The relative standard deviations of absolute migration times determined in repetitive MCE separations of FITC-labelled amines were below 2.5% (n= 25). By the application of cyclodextrin-modified electrolytes, chiral separation of FITC-labelled amines could be performed in seconds demonstrating the potential of microchip electrophoresis for chiral high-throughput screening.

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“…Coatings based on PVA, which has to be considered the most hydrophilic nonionic polymer, have proven to exhibit a particularly good performance in classical CE [46,77]. The use of covalently linked PVA coatings in microfluidic devices was mentioned by Zhang et al [74].…”

Section: Pvamentioning

confidence: 99%

Surface modification in microchip electrophoresis

2003

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Different approaches and techniques for surface modification of microfluidic devices applied for microchip electrophoresis are reviewed. The main focus is on the improved electrophoretic separation by reducing analyte-wall interactions and manipulation of electroosmosis. Approaches and methods for permanent and dynamic surface modification of microfluidic devices, manufactured from glass, quartz and also different polymeric substrates, are described.

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Cross-linked poly(vinyl alcohol) as permanent hydrophilic column coating for capillary electrophoresis

Cited by 123 publications

References 29 publications

Poly(vinyl alcohol)-coated microfluidic devices for high-performance microchip electrophoresis

Poly(vinyl alcohol)-coated microfluidic devices for high-performance microchip electrophoresis

Design and performance of a microchip electrophoresis instrument with sensitive variable-wavelength fluorescence detection

Surface modification in microchip electrophoresis

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