Cross-linking of Bovine Plasma Albumin and Wool Keratin

Chemically Reactive Sulfhydryl Groups of 1 -Aminocyclopropane-1 -carboxylate Deaminase

“…ACPC deaminase (1.1 mg/ml) was incubated with 0.8mM N,N'- (I,4-phenylene)bismaleimide 6 ) at pH 8.5 and 20°C for 90 min. The reaction was stopped by adding an equal volume of 2% p-mercaptoethanol.…”

Cross‐linking of ADP‐ribosylated human translocation factor to ribosomes

“…Incubation in the presence of imidates was done for 20 hr(at 0°C in 50 mM phosphate buffer pH 8.5. These incubation conditions were chosen according to studies described [10][11][12][13][14][15][16][17][18][19][20][21]. Throughout the incubation, the salt concentrations of the homogenization buffer were maintained; the reagent concentration was adjusted to 1 mM, if not indicated otherwise.…”

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“…Selective alkylation of keratinous cysteine was carried out by its conjugate addition to Michael acceptors [36,39]. Moore and Ward [40] brought reduction in disulfide bonds and thereafter replaced the crosslink with bismaleimides adduct. Investigators continued using maleimides to the present day for an effective alkylation during modification of keratinous cysteine [41].…”