Cross-reactive anti-human monoclonal antibodies as a tool for B-cell identification in dogs and pigs

Faldyna, Martin; Samankova, P.; Levá, Lenka; Černý, Jan; Oujezdská, Jana; Rehakova, Zuzana; Sinkora, J

doi:10.1016/j.vetimm.2007.06.022

Cited by 34 publications

(24 citation statements)

References 26 publications

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“…The commercial mAb antihuman CD79 seems to label the intracellular portion of the swine molecule in cytometry but not in immunohistochemistry [75]. It is claimed as a pan-B cell but as ascertained by phenotypes warrants further confirmation.…”

Section: Cd79mentioning

confidence: 94%

Membrane markers of the immune cells in swine: an update

Piriou-Guzylack¹,

2008

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-Besides their breeding value, swine are increasingly used as biomedical models. As reported in three international swine clusters of differentiation (CD) workshops and in the animal homologue section of the last workshop for the determination of human leukocyte differentiation antigens (HLDA 8), characterisation of leukocyte surface antigens by monoclonal antibodies and other molecular studies have determined the cell lineages and blood leukocyte subsets implicated in the immune response, including cell adhesion molecules involved in cell trafficking. This review focusses on the current state of knowledge of porcine leukocyte differentiation and major histocompatibility complex (SLA) molecules. Examples of porcine particularities such as the double-positive T lymphocytes with the phenotype CD4 + CD8 low and CD4 − CD8 low T cell subsets and the persistence of SLA class II after T-lymphocyte activation are illustrated, as well as the shared characteristics of the Artiodactyla group, such as the high proportion of TcR (T cell receptor) T cells in blood and other lymphoid tissues. Furthermore, discrepancies between swine and humans, such as CD16 expression on dendritic cells and CD11b (wCD11R1) tissue distribution are outlined. The rapidly growing information should facilitate manipulation of the swine immune system towards improving disease control, and open new avenues for biomedical research using the pig as a model. cluster of differentiation (CD) / monoclonal antibody / immune system / histocompatibility system / pig

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Section: Cd79mentioning

confidence: 94%

Membrane markers of the immune cells in swine: an update

Piriou-Guzylack¹,

2008

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“…1D6, Serotec), anti-canine CD45-FITC or -PE (YKIX716.13, Serotec), anti-canine CD11/18-FITC (YKIX490.6.4, Serotec), anti-human CD14-PE (TÜK4, Serotec), and unconjugated anti-canine CD11b (CA16.3E10, Serotec), anti-human CD22 (RFB4, Abcam, Cambridge, MA), anti-bovine MHC I (H58A, VMRD, Pullman, WA), anti-human HLA-DR (L243, BD Biosciences, San Jose, CA), anti-human CD94 (HP-3DP, BD Biosciences), anti-human CD56 (B-159, BD Biosciences), anti-human Nkp46 (BAB281, Beckman Coulter, Miami, FL), anti-human TIM-3 (FAB2365P, R&D Systems, Minneapolis, MN). The antibodies against human CD14 (Vernau et al, 1999), CD22 (Faldyna et al, 2007), and CD94 (Schuberth et al, 2007) have been previously verified to recognize the corresponding epitopes of the homologous canine proteins. At least 2000 cells were analyzed.…”

Section: Methodsmentioning

confidence: 99%

Isolation and characterization of canine natural killer cells

Michael

Ito

McCullar

et al. 2013

Veterinary Immunology and Immunopathology

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NK cells are non-T, non-B lymphocytes that kill target cells without previous activation. The immunophenotype and function of these cells in humans and mice are well defined, but canine NK cells remain incompletely characterized. Our objectives were to isolate and culture canine peripheral blood NK cells, and to define their immunophenotype and killing capability. PBMC were obtained from healthy dogs and T cells were depleted by immunomagnetic separation. The residual cells were cultured in media supplemented with IL-2, IL-15 or both, or with mouse embryonic liver (EL) feeder cells. Non-T, non-B lymphocytes survived and expanded in these cultures. IL-2 was necessary and sufficient for survival; the addition of IL-15 was necessary for expansion, but IL-15 alone did not support survival. Culture with EL cells and IL-2 also fostered survival and expansion. The non-T, non-B lymphocytes uniformly expressed CD45, MHC I, and showed significant cytotoxic activity against CTAC targets. Expression of MHC II, and of CD11/18 was restricted to subsets of these cells. The data show that cells meeting the criteria for NK cells in other species, i.e., non-T, non-B lymphocytes with cytotoxic activity, can be expanded from canine PBMC by T-cell depletion and culture with cytokines or feeder cells.

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“…The expression of CD25, CD79α and SWC3 can be also used for identification of B cell precursors in primary lymphopoietic centers (Sinkora et al, 2002). Cross-reactive CD20 and CD86 were also recently shown to be useful for phenotyping of porcine B cells(Faldynaetal., 2007). HoweverB1and B2cells cannot be discriminated by CD5 expression in swine.…”

Section: Characterization Of the B Cell Systemmentioning

confidence: 99%

The piglet as a model for B cell and immune system development

Butler

Lager

Šplı́chal

et al. 2009

Veterinary Immunology and Immunopathology

112

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The ability to identify factors responsible for disease in all species depends on the ability to separate those factors which are environmental from those that are intrinsic. This is particularly important for studies on the development of the adaptive immune response of neonates. Studies on laboratory rodents or primates have been ambiguous because neither the effect of environmental nor maternal factors on the newborn can be controlled in mammals that: (i) transmit potential maternal immunoregulatory factors in utero and (ii) are altricial and cannot be reared after birth without their mothers. Employing the newborn piglet model can address each of these concerns. However, it comes at the price of having first to characterize the immune system of swine and its development. This review focuses on the porcine B cell system, especially on the methods used for its characterization in fetal studies and neonatal piglets. Understanding these procedures is important in the interpretation of the data obtained. Studies on neonatal piglets have (a) provided valuable information on the development of the adaptive immune system, (b) lead to important advances in evolutionary biology, (c) aided our understanding of passive immunity and (d) provided opportunities to use swine to address specific issues in veterinary and biomedical research and immunotherapy. This review summarizes the history of the development of the piglet as a model for antibody repertoire development, thus providing a framework to guide future investigators.

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Cross-reactive anti-human monoclonal antibodies as a tool for B-cell identification in dogs and pigs

Cited by 34 publications

References 26 publications

Membrane markers of the immune cells in swine: an update

Membrane markers of the immune cells in swine: an update

Isolation and characterization of canine natural killer cells

The piglet as a model for B cell and immune system development

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