Murine monoclonal antibodies (MAbs) reacting with Pseudomonas syringae lipopolysaccharide (LPS) O polysaccharides (OPS) composed of tetra-and tri-␣-D-rhamnose repeats in the backbone [3)D-Rha(␣1-3)D-Rha (␣1-2)D-Rha(␣1-2)D-Rha(␣1] and [3)D-Rha(␣1-3)D-Rha(␣1-2)D-Rha(␣1]were generated and used for immunochemical analysis and for serological classification of the bacteria. A total of 195 of 358 P. syringae strains tested representing 21 pathovars were shown to share a common epitope, 1a, and were classified into serogroup O1. All strains with pathovars aptata, glycinea, japonica, phaseolicola, and pisi, most of the strains with pathovars atrofaciens and striafaciens, and half of the strains with pathovar syringae were classified into serotypes O1a, O1b, O1c, and O1d within serogroup O1. Serogroup-specific epitope 1a was inferred to be related to the ( All oxidase-negative and arginine dihydrolase-negative fluorescent bacteria are currently classified as Pseudomonas syringae van Hall. This is the most prevalent species within the pseudomonads (36). More than 50 distinct pathovars have been identified to meet the needs of plant pathologists concerned with the differential pathogenicity of P. syringae strains (41, 50, 51). Although the bacterium was originally isolated from lilacs, the pathovars of P. syringae cause diseases on nearly every cultivated plant and on a hitherto-unknown number of wild plant species. The pathovars of P. syringae are becoming appreciated not only as phytopathogens but also as common epiphytes of healthy host and nonhost plant species, as well as ice nucleation agents capable of inciting frost damage in plants (11,24).The taxonomy (classification, nomenclature, and identification) of P. syringae in general and the trinominal nomenclature in particular are confusing, and it is difficult to identify each pathovar solely on the basis of biochemical and physiological features. Many P. syringae pathovars can infect only specific plant species, whereas others, especially P. syringae pv. syringae, possess a wide host range (3,4,9,41). The ability of medical bacteriologists to provide rapid and accurate serological identification of gram-negative bacteria based on the lipopolysaccharide (LPS) diversity (25,26,30,31) indicates a similar potential for the taxonomy of P. syringae. The serological features of P. syringae have not as yet been studied thoroughly (24). None of the earlier (33,37,40) or recent (10) attempts to create a serological classification scheme of P. syringae has been completed. A limited number of P. syringae strains have been studied with monoclonal antibodies (MAbs) (5,35,43,48), known to be a powerful tool for taxonomic purposes (22,23,29,45). The molecular structure of the O-polysaccharide (OPS) repeating units of LPS have recently been elucidated for many strains of P. syringae (1,2,8,14,32,42). However, the discrepancies between the chemical structures of OPS (14) and the previously proposed serological classification scheme (37) could be clearly noticed. A detailed descriptio...