Cross‐reactivity of anti‐chicken IgY antibody with immunoglobulins of exotic avian species

Cray, Carolyn; Villar, David

doi:10.1111/j.1939-165x.2008.00055.x

Cited by 22 publications

(13 citation statements)

References 14 publications

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“…In this study, the use of anti-macaw IgY secondary antibody in the dot-blot ELISA for testing antibody-positive Blue and gold macaw plasma generated appreciably better visible signals with consistently higher signal intensity than the use of antibird, anti-duck, or anti-chicken IgY secondary antibodies ( Figure 2; Table 1). Though some studies have used antichicken secondary antibody in serologic assays in wild birds, 28,29 our results were in agreement with a study that found the use of anti-passerine IgY secondary antibody in a microplate ELISA performed better than anti-chicken and anti-bird secondary antibodies when testing passerines. 25 In fact, we found that the use of antimacaw IgY secondary antibody, in both the Western blot and the dot-blot ELISAs, showed strong species specificity for psittacines.…”

Section: Discussionsupporting

confidence: 91%

<p>Comparison Of Four Anti-Avian IgY Secondary Antibodies Used In Western Blot And Dot-Blot ELISA To Detect Avian Bornavirus Antibodies In Four Different Bird Species</p>

Escandon¹,

Heatley²,

Berghman³

et al. 2019

VMRR

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PurposeThis study evaluated the specificity of different avian secondary antibodies used in Western blot and dot-blot ELISA to detect avian bornavirus antibodies in bird plasma.MethodsPlasma samples were collected from: two Blue and gold macaws, one positive and one negative for avian bornavirus by RT-PCR; a Cockatiel and a Monk parakeet prior to and following experimental infection; and, two Mallards, one positive and one negative for avian bornavirus by RT-PCR Samples were analyzed by Western blot and dot-blot ELISA that incorporated recombinant avian bornavirus nucleoprotein as the target analyte. Four species-specific anti-IgY secondary antibodies were used in the assays: goat anti-macaw IgY, goat anti-bird IgY, goat anti-duck IgY, and rabbit anti-chicken IgY.ResultsIn the Western blot, anti-macaw IgY secondary antibody produced strong signals with Blue and gold macaw and Cockatiel positive plasma, but no signal with Mallard positive plasma. Anti-bird IgY secondary antibody produced strong signals with Blue and gold macaw, Cockatiel, and Mallard positive plasma. Anti-duck and anti-chicken IgY secondary antibody produced a strong and moderate signal, respectively, only with Mallard positive plasma. In the dot-blot ELISA, there was a distinct and significant difference (P<0.05) in the signal intensity between the different secondary antibodies within a bird species. Anti-macaw IgY secondary antibody produced significantly (P<0.05) stronger signals than the other secondary antibodies in Blue and gold macaw, Cockatiel, and Monk parakeet positive plasma, while anti-duck IgY secondary antibody produced significantly (P<0.05) stronger signals than the other secondary antibodies in Mallard positive plasma.ConclusionIn testing psittacines with immunoassays, and especially in assays that incorporate short incubation reaction times such as a dot-blot ELISA, species-specific anti-IgY secondary antibodies provided more accurate results.

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Section: Discussionsupporting

confidence: 91%

<p>Comparison Of Four Anti-Avian IgY Secondary Antibodies Used In Western Blot And Dot-Blot ELISA To Detect Avian Bornavirus Antibodies In Four Different Bird Species</p>

Escandon¹,

Heatley²,

Berghman³

et al. 2019

VMRR

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“…The serum samples were added at 1:500 dilution and after 30 minutes of the incubation and three washings, were incubated with the anti-chicken IgY peroxidase conjugate (Sigma Chemicals) for 1 h at 37 °C. These anti-chicken IgY antibodies had cross-reactivity with IgY of penguin as demonstrated by ELISA (date not show) and Western blotting (Cray et al 2008). The relative level of antibody was determined by calculating the sample to positive ratio (S/P).…”

Section: Elisa To Ibdvmentioning

confidence: 99%

Investigation on Newcastle Disease Virus (NDV), Infectious Bursal Disease Virus (IBDV) and Avian Poxvirus (APV) in magellanic penguins in Southern region of Brazil

Nunes

Fonseca

Leite

et al. 2012

Braz. arch. biol. technol.

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“…Coefficients of variation were calculated in Microsoft Excel for each individual colony (North Dakota and South Dakota) and as a group so we could compare variation of food consumed. To accurately determine the concentrations of antibodies within the samples, we devised a modified direct (serum) and indirect (regurgitate) ELISA test (Crowther 2001;Martinez et al 2003;Cray and Villar 2008). It was necessary to devise a novel ELISA test due to the uncertainty of tested IgA and IgY ELISA commercial kits regarding non-chicken avian species (Crowther 2001;Martinez et al 2003;Cray and Villar 2008).…”

Section: Discussionmentioning

confidence: 99%

American White Pelican (Pelecanus erythrorhynchos) Growth, Nutrition and Immunology

2019

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Limited information about nutrition exists on American White Pelicans (Pelecanus erythrorhynchos) from hatching to fledging. To detail immunity, metabolism and nutrition of juvenile American White Pelicans, during 22-23 July 2011, 103 samples of regurgitate matter were collected at five Chase Lake, North Dakota, USA, and three Bitter Lake, South Dakota, USA, sub-colonies. Regurgitate sample nutrient content was significantly different for organic matter (P = 0.012), crude protein (P = 0.001), neutral detergent fiber (P = 0.014), acid detergent fiber (P = 0.005) and energy (P = 0.034) between North (n = 5) and South (n = 3) Dakota American White Pelican colonies. Average concentrations of immunoglobulins Y (2.74 ± 1.85 ng/mL) and A (9.04 ± 9.41 ng/mL) demonstrated passive transfer of immunity in regurgitate. To enhance information on growth and morphology in hand-reared American White Pelicans (n = 8), a 9-week captive trial was also conducted raising chicks from hatching to fledging. Predictive models were created to describe chick growth for intake, body weight, culmen length and tarsus length. Data collected during this study enhances both American White Pelican general ecology and conservation with implications for both captive and wild bird management.

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Cross‐reactivity of anti‐chicken IgY antibody with immunoglobulins of exotic avian species

Abstract: The results demonstrate that some commercially available anti-chicken IgY antibodies exhibit good cross-reactivity with most avian species.

Cited by 22 publications

References 14 publications

<p>Comparison Of Four Anti-Avian IgY Secondary Antibodies Used In Western Blot And Dot-Blot ELISA To Detect Avian Bornavirus Antibodies In Four Different Bird Species</p>

<p>Comparison Of Four Anti-Avian IgY Secondary Antibodies Used In Western Blot And Dot-Blot ELISA To Detect Avian Bornavirus Antibodies In Four Different Bird Species</p>

Investigation on Newcastle Disease Virus (NDV), Infectious Bursal Disease Virus (IBDV) and Avian Poxvirus (APV) in magellanic penguins in Southern region of Brazil

American White Pelican (Pelecanus erythrorhynchos) Growth, Nutrition and Immunology

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