Cross‐Reactivity of Anti–HIV‐1 T Cell Immune Responses among the Major HIV‐1 Clades in HIV‐1–Positive Individuals from 4 Continents

Coplan, Paul; Gupta, Swati; Dubey, Sheri; Pitisuttithum, Punnee; Nikas, Alex; Mbewe, Bernard; Vardas, Eftyhia; Schechter, Mauro; Kallas, Esper G.; Freed, Dan; Fu, Tong Ming; Mast, T. Christopher; Puthavathana, Pilaipan; Kublin, James G.; Collins, Kathryn S.; Chisi, John; Pendame, Richard; Thaler, Scott J.; Gray, Glenda; McIntyre, James; Straus, Walter L.; Condra, Jon H.; Mehrotra, Devan V.; Guess, Harry A.; Emini, Emilio A.; Shiver, John W.

doi:10.1086/428450

Cited by 69 publications

(35 citation statements)

References 37 publications

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“…7C) most likely the consequence of the internalization of shed viral proteins. This assay was significant because Gag and Nef proteins are expected to be the viral products most commonly recognized by effector lymphocytes from HIV-1-infected patients [25][26][27][28]. We confirmed this finding by evaluating the HIV-1 Ag-presenting activity of iDC infected with WT or Denv HIV-1 in the presence of AZT (data not shown).…”

Section: High Levels Of Hiv-1 Endocytosis Correlate With Strong Hiv-1supporting

confidence: 61%

DC contact with HIV‐1‐infected cells leads to high levels of Env‐mediated virion endocytosis coupled with enhanced HIV‐1 Ag presentation

et al. 2009

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In HIV-infected patients, DC are likely to interact with both cell-free HIV and HIV-infected cells. We were interested in investigating the mechanism of virus transmission occurring upon contact between HIV-1-infected cells and DC, as well as the consequences for HIV-1 Ag-presenting activity. By comparing mixed co-cultures with trans-well cultures, we observed that cell-to-cell contact strongly increased HIV-1 Env-mediated virion endocytosis in target DC. This endocytosis was independent of HIV-1 tropism, de novo infection, HIV-1 Env-CD4-dependent fusion, and immature DC activation/maturation. We also found that augmentation of HIV-1 endocytosis was closely correlated with strong, Env-dependent HIV-1 Ag presentation by DC. Our results provide a better understanding of the mechanisms underlying the induction of the anti-HIV adaptive immune response. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim

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Section: High Levels Of Hiv-1 Endocytosis Correlate With Strong Hiv-1supporting

confidence: 61%

DC contact with HIV‐1‐infected cells leads to high levels of Env‐mediated virion endocytosis coupled with enhanced HIV‐1 Ag presentation

et al. 2009

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“…Putatively, the generation of consensus sequences will erase functional HLA anchor residues and thus explain the distinction seen between the peptide pools. However, in a large recently conducted study (250 patients) (16), intraclade responses were found to be higher than interclade responses using the same reagents, leading to a probable dilution of the aforementioned phenomenon. These results prompted us to analyze amino acid variation in reported CTL epitopes.…”

Section: Discussionmentioning

confidence: 87%

Broad Cross-Clade T-Cell Responses to Gag in Individuals Infected with Human Immunodeficiency Virus Type 1 Non-B Clades (A to G): Importance of HLA Anchor Residue Conservation

Geels

Dubey

Anderson

et al. 2005

J Virol

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“…While CD8 ϩ T-cell cross-clade recognition has been tested extensively (6,11,19,36,48), few studies have addressed the possibility of clade-specific escape from CD8 ϩ T-cell responses. This may be especially relevant where clade consensus sequences differ in immunologically relevant epitopes.…”

Section: Hiv-specific Cd8mentioning

confidence: 99%

Clade-Specific Evolution Mediated by HLA-B*57/5801 in Human Immunodeficiency Virus Type 1 Clade A1 p24

McKinnon

Capiña

Peters

et al. 2009

J Virol

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HLA-B*57-mediated selection pressure leads to a typical escape pathway in human immunodeficiency virus type 1 (HIV-1) CD8 epitopes such as TW10. Whether this T242N pathway is shared by all clades remains unknown. We therefore assessed the nature of HLA-B*57 selection in a large, observational Kenyan cohort where clades A1 and D predominate. While T242N was ubiquitous in clade D HLA-B*57 ؉ subjects, this mutation was rare (15%) in clade A1. Instead, P243T and I247L were selected by clade A1-infected HLA-B*57 subjects but not by HLA-B*5801 ؉ subjects. Our data suggest that clade A1 consensus proline at Gag residue 243 might represent an inherent block to T242N escape in clade A1. We confirmed immunologically that P243T and I247L likely represent escape mutations. HLA-B*57 evolution also differed between clades in the KF11 and IW9 epitopes. A better understanding of clade-specific evolution is important for the development of HIV vaccines in regions with multiple clades.

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Cross‐Reactivity of Anti–HIV‐1 T Cell Immune Responses among the Major HIV‐1 Clades in HIV‐1–Positive Individuals from 4 Continents

Abstract: Cross-clade reactivity of cellular immune responses can be substantial but varies by viral protein.

Cited by 69 publications

References 37 publications

DC contact with HIV‐1‐infected cells leads to high levels of Env‐mediated virion endocytosis coupled with enhanced HIV‐1 Ag presentation

DC contact with HIV‐1‐infected cells leads to high levels of Env‐mediated virion endocytosis coupled with enhanced HIV‐1 Ag presentation

Broad Cross-Clade T-Cell Responses to Gag in Individuals Infected with Human Immunodeficiency Virus Type 1 Non-B Clades (A to G): Importance of HLA Anchor Residue Conservation

Clade-Specific Evolution Mediated by HLA-B*57/5801 in Human Immunodeficiency Virus Type 1 Clade A1 p24

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