Cross talk between heat shock protein 10 and a heat shock factor identified from Marsupenaeus japonicus

Zheng, Jinbin; Mao, Yong; Su, Yongquan; Wang, Jun

doi:10.1016/j.ijbiomac.2019.10.072

Cited by 12 publications

(6 citation statements)

References 59 publications

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“…These results are consistent with findings reported by Guo (2013) who found that Bthsf in Bemisia tabaci can be induced by low but not high temperatures [41]. In contrast, hsf in Marsupenaeus japonicus was induced in response to heat stress [42], which suggests that hsf expression patterns vary in different insect species. In general, there are relatively few studies on the expression pattern of insect HSFs during temperature stress.…”

Section: Discussionsupporting

confidence: 92%

Molecular Characterization of Heat-Induced HSP11.0 and Master-Regulator HSF from Cotesia chilonis and Their Consistent Response to Heat Stress

Zhu

et al. 2021

Insects

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Small heat shock proteins (sHSPs) are members of the heat shock protein (HSP) family that play an important role in temperature stress, and heat shock factors (HSFs) are transcriptional activators that regulate HSP expression. Cotesia chilonis, the major endoparasitoid of Chilo suppressalis, modulates the C. suppressalis population in the field. In this study, we cloned and characterized two genes from C.chilonis: the heat-induced HSP11.0 gene (Cchsp11.0) that consisted of a 306-bp ORF, and the master regulator HSF (Cchsf) containing an 1875-bp ORF. CcHSP11.0 contained a chaperonin cpn10 signature motif that is conserved in other hymenopteran insects. CcHSF is a typical HSF and contains a DNA-binding domain, two hydrophobic heptad repeat domains, and a C-terminal trans-activation domain. Neither Cchsp11.0 or Cchsf contain introns. Real-time quantitative PCR revealed that Cchsp11.0 and Cchsf were highly induced at 36 °C and 6 °C after a 2-h exposure. Overall, the induction of Cchsf was lower than Cchsp11.0 at low temperatures, whereas the opposite was true at high temperatures. In conclusion, both Cchsp11.0 and Cchsf are sensitive to high and low temperature stress, and the expression pattern of the two genes were positively correlated during temperature stress.

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Section: Discussionsupporting

confidence: 92%

Molecular Characterization of Heat-Induced HSP11.0 and Master-Regulator HSF from Cotesia chilonis and Their Consistent Response to Heat Stress

Zhu

et al. 2021

Insects

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“…The concentration and quality of total RNA were examined by NanoDrop™ One C Microvolume UV–Vis Spectrophotometer (Thermo Fisher Scientific). Cloning of the full‐length cDNA sequences of sHSPs was performed as previously reported (Zheng et al, 2020). All primers used for PCR are listed in Table 1.…”

Section: Methodsmentioning

confidence: 99%

“…The eluent was collected and the purity of the recombinant proteins was analysed by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) and Coomassie Blue staining. The recombinant proteins were confirmed by Western blot analysis as previously described (Zheng et al, 2020). Briefly, the recombinant proteins solutions were separated by SDS‐PAGE and transferred onto polyvinylidene fluoride (PVDF) membranes.…”

Section: Methodsmentioning

confidence: 99%

“…Primers for qRT-PCR (Table 1) were designed based on the full-length cDNA sequence of the three sHSPs. The PCR efficiency was calculated as previously reported, and qRT-PCR was conducted according to previous studies (Zheng et al, 2020). Five biological and three technical replicates were performed, and β-actin was used as internal control.…”

Section: Real-time Quantitative Pcr (Qrt-pcr)mentioning

confidence: 99%

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Bacteria binding and antibacterial activities of three small heat shock protein from the swimming crab, Portunus trituberculatus

Zhang

Zheng

et al. 2022

Aquaculture Research

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The swimming crab, Portunus trituberculatus, is one of the most important commercial crabs cultured in China, with a total yield of approximately 100,000 tons (Fisheries Bureau of Agriculture Ministry of China, 2019). In recent decade, despite the increasing development of the P. trituberculatus farming industry, diseases caused by bacterial infection have caused tremendous economic losses and posed serious threats to the sustainable and healthy development of P. trituberculatus aquaculture industry. Excavating immune-related molecules from host, especially antibacterial molecules, will provide theoretical guidance and candidate therapeutic agents to address the disease problem.Heat shock proteins (HSPs), which widely exist in prokaryotes and eukaryotes, are molecular chaperones that play critical roles in maintaining cellular homeostasis (Schlesinger, 1990;Sikora & Grzesiuk, 2007). According to their homology and molecular mass, HSPs can be grouped into several families, including HSP110, HSP100, HSP90, HSP70, HSP60 and small molecular mass HSPs (sHSPs) (Craig et al., 1994;Tang et al., 2011). HSPs play a primary role

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“…The expression levels of HSPs and other heat tolerance related genes in P. monodon changed significantly after the PmHSF1 gene was knocked down (Sornchuer et al, 2018). In M. japonicas, MjHSF1 transcription was upregulated under heat stress (Zheng et al, 2020). To date, most of the studies on the related functions of HSF1 have focused on the interaction between HSF1 and HSPs, and there are few studies on the expression of immune related factors associated with HSF1.…”

Section: Effects Of Low Temperature On the Antioxidant Systemmentioning

confidence: 99%

Effects of Low Temperature on Shrimp and Crab Physiology, Behavior, and Growth: A Review

et al. 2021

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As important aquaculture species worldwide, shrimps and crabs are thermophilic animals with a feeble thermoregulation ability. Changes in environmental factors are the main reason for the decrease in the immunity and disease resistance ability of cultured organisms. Water temperature is one of the most common abiotic stress factors for aquatic ectotherms. It influences nearly all biochemical and physiological processes in crustaceans, resulting in an imbalance in ion and water homeostasis, neuromuscular function loss, cellular dehydration, and altered metabolic pathways. The present review summarizes the current knowledge on the effects of low temperature on the physiological response, and the behavior, development, and growth of shrimp and crab. We suggest a deeper research to understand the physiological processes involved in thermoregulation; this knowledge could be used to reduce the adverse effects in the shrimps and crabs during the culture.

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Cross talk between heat shock protein 10 and a heat shock factor identified from Marsupenaeus japonicus

Cited by 12 publications

References 59 publications

Molecular Characterization of Heat-Induced HSP11.0 and Master-Regulator HSF from Cotesia chilonis and Their Consistent Response to Heat Stress

Molecular Characterization of Heat-Induced HSP11.0 and Master-Regulator HSF from Cotesia chilonis and Their Consistent Response to Heat Stress

Bacteria binding and antibacterial activities of three small heat shock protein from the swimming crab, Portunus trituberculatus

Effects of Low Temperature on Shrimp and Crab Physiology, Behavior, and Growth: A Review

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