Cross-transferability analysis of SSR markers developed from the American Cranberry (Vaccinium macrocarpon Ait.) to other Vaccinium species of agricultural importance

The Genetic Diversity of Cranberry Crop Wild Relatives, Vaccinium macrocarpon Aiton and V. oxycoccos L., in the US, with Special Emphasis on National Forests

“…The number of private alleles per population of V. macrocarpon ranged from 0 to 26. The highest numbers of private alleles were found in populations at Red Run Bog in the Monongahela National Forest in West Virginia (26), Upper Island Lake in the Chequamegon-Nicolet National Forest in Wisconsin (24), Sand Point Bog in the Keweenaw Bay Indian Community in Michigan (23), and Cranberry Glades Site 5 in the Monongahela National Forest (17) (Figure 3). A PCA was performed to understand the genetic relationships among V. macrocarpon populations (Figure 4).…”

“…Molecular markers such as simple sequence repeats (SSR) have been identified for many plant species and successfully used to gain insights into the genetic diversity of CWR of several crops, including apple [18], wheat [19], sugarcane [20], and sweet potato [21]. In cranberry, SSR markers have been used to evaluate the genetic diversity of wild populations in Wisconsin and Minnesota [22,23] and have proven to be transferable across species in the genus Vaccinium [24], which will also facilitate interspecific breeding and fingerprinting. Results from genetic diversity analyses have provided sufficient information to aid in situ conservation efforts in several plant species, such as Borderea chouardii Gaussen and Heslot, an endangered plant in Spain [25], and wild potatoes in Argentina [26].…”

Exploring the Genetic Diversity of Wild Cranberry Populations in the Upper Midwestern United States

“…The pellet was then washed with 700 ml of cold 70% ethanol to clean the DNA. The solution was then vortexed and centrifuged at 14,000 rpm for 4 min, the ethanol was discarded, and the pellet was air dried for 24 h. The DNA was then resuspended in 100 ml TE 10:1 buffer (10 mM Tris-HCl pH 8.0, 1 mM EDTA pH 8.0) plus 5 ml of ribonuclease (RNase 10 mg ml −1 ) in each tube and was incubated at 37°C for 2 h prior in multiple studies, and were the best markers among 697 SSRs tested across different cranberry samples and other Vaccinium species (Schlautman et al, 2015a(Schlautman et al, , 2015b(Schlautman et al, , 2017(Schlautman et al, , 2018Rodriguez-Bonilla et al, 2019). Since all the markers used in this study were previously used in cranberry linkage mapping, the markers have been proven to segregate in a Mendelian fashion (Schlautman et al, 2015a(Schlautman et al, , 2015b(Schlautman et al, , 2017(Schlautman et al, , 2018.…”

“…The multiplex panels were designed by developing SSR primer pairs. These pairs were clustered into combinations of one to three markers, considering nonoverlapping fragment (i.e., alleles) ranges and annealing temperatures based on previous studies (Georgi et al, 2012;Zhu et al, 2012;Schlautman et al, 2015aSchlautman et al, , 2015bSchlautman et al, , 2017Schlautman et al, , 2018Rodriguez-Bonilla et al, 2019). To boost to storage at −20°C.…”

“…A total of 32 markers (supplemental materials) previously designed and assessed for transferability between V. macrocarpon and V. oxycoccos were used in this study (Schlautman et al, 2015a(Schlautman et al, , 2015bRodriguez-Bonilla et al, 2019). Since we are amplifying the markers now in two closely related cranberry species, one of the disadvantages with SSRs is that if a polymorphism is obtained it may not necessarily be due to corresponding homologous chromosomes, particularly when working with polyploids, but due to nonspecific amplification of nonhomologous chromosomes.…”