Exploring the Genetic Diversity of Wild Cranberry Populations in the Upper Midwestern United States

Rodríguez-Bonilla, Lorraine; Bonilla, Fabian Rodríguez; Matusinec, Daniel; Wiesman, Eric; Schoville, Sean D.; Atucha, Amaya; Zalapa, Juan

doi:10.2135/cropsci2019.06.0367

Cited by 7 publications

(9 citation statements)

References 63 publications

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“…Clear separation between the two species has been previously observed using amplified fragment length polymorphism (AFLP) [34] and SSR markers [22]. Rodriguez-Bonilla et al [23] also observed a clear differentiation between species collected in natural areas in the states of Wisconsin and Minnesota, demonstrating the ability of these markers to differentiate between closely related species. 2), which are comparable to those found by Zalapa et al [22] and Rodriguez-Bonilla et al [23], who found high levels of heterozygosity in wild populations in both Wisconsin and Minnesota.…”

Section: Genetic Analysis Shows High Differentiation Between Morphologically Similar Speciesmentioning

confidence: 75%

“…Molecular markers such as simple sequence repeats (SSR) have been identified for many plant species and successfully used to gain insights into the genetic diversity of CWR of several crops, including apple [18], wheat [19], sugarcane [20], and sweet potato [21]. In cranberry, SSR markers have been used to evaluate the genetic diversity of wild populations in Wisconsin and Minnesota [22,23] and have proven to be transferable across species in the genus Vaccinium [24], which will also facilitate interspecific breeding and fingerprinting. Results from genetic diversity analyses have provided sufficient information to aid in situ conservation efforts in several plant species, such as Borderea chouardii Gaussen and Heslot, an endangered plant in Spain [25], and wild potatoes in Argentina [26].…”

Section: Introductionmentioning

confidence: 99%

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The Genetic Diversity of Cranberry Crop Wild Relatives, Vaccinium macrocarpon Aiton and V. oxycoccos L., in the US, with Special Emphasis on National Forests

Rodríguez-Bonilla

Williams

Bonilla

et al. 2020

Plants

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Knowledge of the genetic diversity in populations of crop wild relatives (CWR) can inform effective strategies for their conservation and facilitate utilization to solve agricultural challenges. Two crop wild relatives of the cultivated cranberry are widely distributed in the US. We studied 21 populations of Vaccinium macrocarpon Aiton and 24 populations of Vaccinium oxycoccos L. across much of their native ranges in the US using 32 simple sequence repeat (SSR) markers. We observed high levels of heterozygosity for both species across populations with private alleles ranging from 0 to 26. For V. macrocarpon, we found a total of 613 alleles and high levels of heterozygosity (HO = 0.99, HT = 0.75). We also observed high numbers of alleles (881) and levels of heterozygosity (HO = 0.71, HT = 0.80) in V. oxycoccos (4x). Our genetic analyses confirmed the field identification of a native population of V. macrocarpon on the Okanogan-Wenatchee National Forest in the state of Washington, far outside the previously reported range for the species. Our results will help to inform efforts of the United States Department of Agriculture Agricultural Research Service (USDA-ARS) and the United States Forest Service (USFS) to conserve the most diverse and unique wild cranberry populations through ex situ preservation of germplasm and in situ conservation in designated sites on National Forests.

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Section: Genetic Analysis Shows High Differentiation Between Morphologically Similar Speciesmentioning

confidence: 75%

Section: Introductionmentioning

confidence: 99%

The Genetic Diversity of Cranberry Crop Wild Relatives, Vaccinium macrocarpon Aiton and V. oxycoccos L., in the US, with Special Emphasis on National Forests

Rodríguez-Bonilla

Williams

Bonilla

et al. 2020

Plants

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“…The analyses presented are strongly supported by genome-wide sequence variation revealed by more than 21,000 SNPs, the largest set of markers ever used in cranberry (Figure 1). Compared to previous diversity studies in cranberry (Fajardo et al, 2013;Zalapa et al, 2015;Schlautman et al, 2017a;Schlautman et al, 2018;Rodríguez-Bonilla et al, 2019), both the number of accessions examined and the number of genetic markers are greatly increased.…”

Section: Discussionmentioning

confidence: 99%

“…Cranberry genetics studies that have contributed to the ongoing development of markerbreeding strategies include a genome assembly (Polashock et al, 2014), several high-density linkage maps (Schlautman et al, 2015;Covarrubias-Pazaran et al, 2016;Schlautman et al, 2017a), QTL mapping of fruit rot resistance (Georgi et al, 2013;Daverdin et al, 2017), fruit size and shape (Diaz-Garcia et al, 2018a), and fruit metabolites (Diaz-Garcia et al, 2018b;Fong et al, 2020), and genomic selection studies (Covarrubias-Pazaran et al, 2018). Additionally, only a few studies have investigated the genetic diversity in cranberry cultivars, cranberry wild populations, and native populations (Fajardo et al, 2013;Zalapa et al, 2015;Schlautman et al, 2017b;Schlautman et al, 2018;Rodríguez-Bonilla et al, 2019), most of them based on a limited number of genetic markers.…”

Section: Introductionmentioning

confidence: 99%

Genotyping-by-Sequencing Identifies Historical Breeding Stages of the Recently Domesticated American Cranberry

et al. 2020

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The cranberry (Vaccinium macrocarpon Ait.) is a North American fruit crop domesticated less than 200 years ago. The USDA began the first cranberry breeding program in response to false-blossom disease in 1929, but after the first generation of cultivars were released in the 1950s, the program was discontinued. Decades later, renewed efforts for breeding cranberry cultivars at Rutgers University and the University of Wisconsin yielded the first modern cultivars in the 2000’s. Phenotypic data suggests that current cultivars have changed significantly in terms of fruiting habits compared to original selections from endemic populations. However, due to the few breeding and selection cycles and short domestication period of the crop, it is unclear how much cultivated germplasm differs genetically from wild selections. Moreover, the extent to which selection for agricultural superior traits has shaped the genetic and phenotypic variation of cranberry remains mostly obscure. Here, a historical collection composed of 362 accessions, spanning wild germplasm, first-, second-, and third-generation selection cycles was studied to provide a window into the breeding and domestication history of cranberry. Genome-wide sequence variation of more than 20,000 loci showed directional selection across the stages of cranberry domestication and breeding. Diversity analysis and population structure revealed a partially defined progressive bottleneck when transitioning from early domestication stages to current cranberry forms. Additionally, breeding cycles correlated with phenotypic variation for yield-related traits and anthocyanin accumulation, but not for other fruit metabolites. Particularly, average fruit weight, yield, and anthocyanin content, which were common target traits during early selection attempts, increased dramatically in second- and third-generation cycle cultivars, whereas other fruit quality traits such as Brix and acids showed comparable variation among all breeding stages. Genome-wide association mapping in this diversity panel allowed us to identify marker-trait associations for average fruit weight and fruit rot, which are two traits of great agronomic relevance today and could be further exploited to accelerate cranberry genetic improvement. This study constitutes the first genome-wide analysis of cranberry genetic diversity, which explored how the recurrent use of wild germplasm and first-generation selections into cultivar development have shaped the evolutionary history of this crop species.

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“…Three papers focused on specific crops such as carrots, lettuce and cranberry provide an important speciesspecific approach to plant conservation (Mezghani et al, 2019;Lebeda et al, 2019;Rodríguez-Bonilla et al, 2019). A crop-specific approach can highlight conservation priorities as well as persisting gaps in species-specific knowledge of taxonomy, crossability, distribution and trait characterizations.…”

Section: Why Connect Agriculture Public Gardens and Science?mentioning

confidence: 99%

Crop Science Special Issue: Connecting Agriculture, Public Gardens and Science

Novy

Moreau

2019

Crop Science

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Exploring the Genetic Diversity of Wild Cranberry Populations in the Upper Midwestern United States

Cited by 7 publications

References 63 publications

The Genetic Diversity of Cranberry Crop Wild Relatives, Vaccinium macrocarpon Aiton and V. oxycoccos L., in the US, with Special Emphasis on National Forests

The Genetic Diversity of Cranberry Crop Wild Relatives, Vaccinium macrocarpon Aiton and V. oxycoccos L., in the US, with Special Emphasis on National Forests

Genotyping-by-Sequencing Identifies Historical Breeding Stages of the Recently Domesticated American Cranberry

Crop Science Special Issue: Connecting Agriculture, Public Gardens and Science

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