1993
DOI: 10.1007/bf01839217
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Crossed immunoelectrophoresis of fungal antigens in tissues as a means of diagnosing systemic aspergillosis and zygomycosis in cattle

Abstract: A novel method for diagnosing bovine aspergillosis and zygomycosis is described. Rabbit hyperimmune antisera raised against somatic antigens of Aspergillus fumigatus and Absidia corymbifera were used in crossed immunoelectrophoresis with supernatants from disintegrated tissues from acute necrohaemorrhagic mycotic lesions from cattle. The method specifically identified 4 of 5 lesions with aspergillosis and 2 of 5 lesions with zygomycosis. One lesion dually infected with aspergillosis and zygomycosis was negativ… Show more

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Cited by 5 publications
(2 citation statements)
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“…Microscopic examinations are especially important in cases where other diagnostic strategies have failed, e.g. mycological culture, antibody or antigen tests, chemical, and molecular biological tests (Hotchi 1967;Jensen 1993Jensen , 1994Kaufman 1992b;Kobayashi et al 1988a;LatgP et al 199 1 ;Lehmann & White 1975;Spreadbury et al 1993;Wong & Brauer 1988;Wong et al 1989).…”
mentioning
confidence: 99%
“…Microscopic examinations are especially important in cases where other diagnostic strategies have failed, e.g. mycological culture, antibody or antigen tests, chemical, and molecular biological tests (Hotchi 1967;Jensen 1993Jensen , 1994Kaufman 1992b;Kobayashi et al 1988a;LatgP et al 199 1 ;Lehmann & White 1975;Spreadbury et al 1993;Wong & Brauer 1988;Wong et al 1989).…”
mentioning
confidence: 99%
“…from stomach contents of goats. The stomach contents are usually a good source for isolation of mycotic agents (Jensen, 1990 andJensen et al, 1994 ). 5), most species of Campylobacter fetus subsp.…”
Section: Discussionmentioning
confidence: 99%