Crosslinked Hyaluronic Acid Gels with Blood-Derived Protein Components for Soft Tissue Regeneration

Hinsenkamp, Adél; Ézsiás, Bence; Pál, Éva; Hricisák, László; Fülöp, Ágnes; Besztercei, Balázs; Somkuti, Judit; Smeller, László; Pinke, Balázs; Kardos, Dorottya; Simon, Melinda; Lacza, Zsombor; Hornyák, István

doi:10.1089/ten.tea.2020.0197

Cited by 13 publications

(15 citation statements)

References 54 publications

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“…The in vitro degradation was analyzed in our previous work using both divinyl sulfone (DVS) and butanediol diglycidyl ether (BDDE) in two relevant concentrations. We found that BDDE is slightly more resistant to hyaluronidase-initiated degradation compared to DVS if they are used in the same concentration [ 20 ]; however, further modification can enable significantly more resistance, as described. In the case of biodegradable hydrogels, the aim is to create a scaffold which resists fast degradation; however, it can be remodeled by attaching cells while new tissue is formed and, preferably, it promotes the vascularization of the implanted hydrogel [ 20 ].…”

Section: Introductionmentioning

confidence: 92%

“…It does not induce cellular attachment and proliferation on the hydrogel [ 22 ]. Hybrid hydrogels may be fabricated to promote cellular attachment, for example, with fibrin [ 20 , 23 ]. Fibrinogen and fibrin are essential for several biological functions in the human body, for example, in hemostasis and wound healing [ 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

“…In our previous investigations, we discovered that the natural clotting process that occurs in whole blood also occurs in plasma and cryoprecipitate, as well as in purified fibrinogen, and these can be combined with biomaterials suitable for scaffold preparation [ 20 , 28 ]. In the present study, we aimed to test the biocompatibility, biodegradability, remodeling, and vascularization of differently crosslinked fibrin containing hyaluronic acid hydrogels in vivo in C57BL/6 mice, based on a previous study [ 20 ]. The cell attachment capacity of the hydrogels was improved by fibrin polymerization into the crosslinked HA matrices.…”

Section: Introductionmentioning

confidence: 99%

“…The cell attachment capacity of the hydrogels was improved by fibrin polymerization into the crosslinked HA matrices. The fibrinogen originated from human FFP, and its concentration in the plasma was augmented by cryoprecipitate isolation [ 20 ]. Butanediol diglycidyl ether and divinyl sulfone were used as crosslinking reagents, two chemical crosslinkers acting on the hydroxyl groups of HA [ 32 , 33 ].…”

Section: Introductionmentioning

confidence: 99%

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Application of Injectable, Crosslinked, Fibrin-Containing Hyaluronic Acid Scaffolds for In Vivo Remodeling

Hinsenkamp

Fülöp

Hricisák

et al. 2022

JFB

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The present research aimed to characterize soft tissue implants that were prepared with the use of crosslinked hyaluronic acid (HA) using two different crosslinkers and multiple reagent concentrations, alone or in combination with fibrin. The effect of the implants was evaluated in an in vivo mouse model, after 4 weeks in one group and after 12 weeks in the other. The explants were compared using analytical methods, evaluating microscopic images, and a histology analysis. The kinetics of the degradation and remodeling of explants were found to be greatly dependent on the concentration and type of crosslinker; generally, divinyl sulfone (DVS) resists degradation more effectively compared to butanediol diglycidyl ether (BDDE). The presence of fibrin enhances the formation of blood vessels, and the infiltration of cells and extracellular matrix. In summary, if the aim is to create a soft tissue implant with easier degradation of the HA content, then the use of 2–5% BDDE is found to be optimal. For a longer degradation time, 5% DVS is the more suitable crosslinker. The use of fibrin was found to support the biological process of remodeling, while keeping the advances of HA in void filling, enabling the parallel degradation and remodeling processes.

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Section: Introductionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Application of Injectable, Crosslinked, Fibrin-Containing Hyaluronic Acid Scaffolds for In Vivo Remodeling

Hinsenkamp

Fülöp

Hricisák

et al. 2022

JFB

Self Cite

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show abstract

“…It can also be applied as a three-dimensional scaffold in tissue engineering [ 17 ] because of its biocompatibility, controllable biodegradability, cell attachment promoting properties [ 18 ], and growth factor content [ 19 ]. Platelet-rich fibrin (PRF) can be isolated from serum immediately after blood drawing into glass tubes [ 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Cell Attachment Capacity and Compounds of Fibrin Membranes Isolated from Fresh Frozen Plasma and Cryoprecipitate

et al. 2021

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Fibrin membranes are widely used in regenerative medicine because they are biocompatible, biodegradable, contain growth factors, and support cell attachment. Most commonly they are produced from serum, but they can also be isolated from activated plasma. To increase the fibrinogen concentration of plasma, cryoprecipitate isolation is a possible solution. In this work, cryoprecipitate was prepared from fresh frozen plasma, isolated by plasmapheresis. The concentration of cellular elements, fibrinogen, total protein, and immunoglobulins among others was measured in different concentrations of cryoprecipitates. After activation with Ca-gluconate, fibrin membranes were produced in different thicknesses, and human mesenchymal stem cells were seeded onto the membranes. They were visualized by live-dead staining and their viability was determined by XTT. The platelet-derived growth factor AB content was quantified by ELISA. Our results showed that fibrinogen and platelet concentration can be multiplied in plasma by cryoprecipitate isolation, which affects the thickness and slightly the growth factor content of the membranes. According to live-dead staining, the thickness of the membranes does not influence cell attachment, and XTT measurement did not reveal a significant difference in cell attachment capacity either; however, a growing trend could be observed in the case of some membranes.

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Modification and preparation of four natural hydrogels and their application in biopharmaceutical delivery

Dong

et al. 2022

Polym. Bull.

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Crosslinked Hyaluronic Acid Gels with Blood-Derived Protein Components for Soft Tissue Regeneration

Cited by 13 publications

References 54 publications

Application of Injectable, Crosslinked, Fibrin-Containing Hyaluronic Acid Scaffolds for In Vivo Remodeling

Application of Injectable, Crosslinked, Fibrin-Containing Hyaluronic Acid Scaffolds for In Vivo Remodeling

Cell Attachment Capacity and Compounds of Fibrin Membranes Isolated from Fresh Frozen Plasma and Cryoprecipitate

Modification and preparation of four natural hydrogels and their application in biopharmaceutical delivery

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