Crosslinking strategies for preparation of extracellular matrix-derived cardiovascular scaffolds

Ma, Bing; Wang, Xiaoya; Wu, Chengtie; Chang, Jiang

doi:10.1093/rb/rbu009

Cited by 158 publications

(98 citation statements)

References 74 publications

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“…The responsive drug release of CCM-Cur by 10 mM GSH treatment may facilitate the intracellular delivery of loaded molecules and drug accumulation in tumors. 24,31,41 Besides, the different release behaviors of NCM-Cur and CCM-Cur at pH 5.0 was attributed to the pyridine ring of NCM, which would be deprotonated to the acid stimuli and thus accelerated Cur release.…”

mentioning

confidence: 99%

Folic acid-targeted disulfide-based cross-linking micelle for enhanced drug encapsulation stability and site-specific drug delivery against tumors

Zhou¹,

Yang²,

Wang³

et al. 2016

IJN

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Although the shortcomings of small molecular antitumor drugs were efficiently improved by being entrapped into nanosized vehicles, premature drug release and insufficient tumor targeting demand innovative approaches that boost the stability and tumor responsiveness of drug-loaded nanocarriers. Here, we show the use of the core cross-linking method to generate a micelle with enhanced drug encapsulation ability and sensitivity of drug release in tumor. This kind of micelle could increase curcumin (Cur) delivery to HeLa cells in vitro and improve tumor accumulation in vivo. We designed and synthesized the core cross-linked micelle (CCM) with polyethylene glycol and folic acid-polyethylene glycol as the hydrophilic units, pyridyldisulfide as the cross-linkable and hydrophobic unit, and disulfide bond as the cross-linker. CCM showed spherical shape with a diameter of 91.2 nm by the characterization of dynamic light scattering and transmission electron microscope. Attributed to the core cross-linking, drug-loaded CCM displayed higher Nile Red or Cur-encapsulated stability and better sensitivity to glutathione than noncross-linked micelle (NCM). Cellular uptake and in vitro antitumor studies proved the enhanced endocytosis and better cytotoxicity of CCM-Cur against HeLa cells, which had a high level of glutathione. Meanwhile, the folate receptor-mediated drug delivery (FA-CCM-Cur) further enhanced the endocytosis and cytotoxicity. Ex vivo imaging studies showed that CCM-Cur and FA-CCM-Cur possessed higher tumor accumulation until 24 hours after injection. Concretely, FA-CCM-Cur exhibited the highest tumor accumulation with 1.7-fold of noncross-linked micelle Cur and 2.8-fold of free Cur. By combining cross-linking of the core with active tumor targeting of FA, we demonstrated a new and effective way to design nanocarriers for enhanced drug encapsulation, smart tumor responsiveness, and elevated tumor accumulation.

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mentioning

confidence: 99%

Folic acid-targeted disulfide-based cross-linking micelle for enhanced drug encapsulation stability and site-specific drug delivery against tumors

Zhou¹,

Yang²,

Wang³

et al. 2016

IJN

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“…39,40 Substrates of low stiffness are more favorable for the induction of ADSC differentiation into an NP-like phenotype. 22 As the concentration of genipin increases, the binding sites of integrin b1 on collagen scaffold reduce and the activation of integrin b1 decreases, which leads to the decrease of ADSCs proliferation and differentiation into an NP-like phenotype. This is likely to be the cause of increased Col1 and Gdf10 expressions.…”

Section: Discussionmentioning

confidence: 99%

“…15 Biological cross-linking is another form of cross-linking which can be carried out under physiological conditions with enzymatical crosslinking agent. 20,21 Genipin is obtained from the natural compound geniposide, which is extracted from the fruit of the Gardenia jasminoides Ellis plant 22 and is regarded as a kind of natural cross-linking agent. 16 But enzymatical cross-linking procedure could also cause unpredictable consequences in specific conditions.…”

Section: Introductionmentioning

confidence: 99%

Genipin‐cross‐linked type II collagen scaffold promotes the differentiation of adipose‐derived stem cells into nucleus pulposus‐like cells

Zhou

Tao

Chen

et al. 2018

J Biomedical Materials Res

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Adipose-derived stem cells (ADSCs)-based tissue engineering was a promising method to treat intervertebral disc degeneration. Type II collagen is a native component in the nucleus pulposus (NP), and has the ability to promote ADSCs to differentiate into NP-like cells. In this article, we aimed to establish a genipin-cross-linked three-dimensional (3D) type II collagen scaffold, and determine the biological effects of the scaffold on ADSCs differentiating into a NP-like phenotype. Different concentrations of genipin were used to cross-link the 3D type II collagen scaffold. Microstructure, surface topography, mechanical strength, porosity, swelling property, and biological stability of the scaffolds were detected to evaluate the scaffold properties. Cell proliferation, gene and protein expression were measured to access the biological effects of the scaffolds on ADSCs, and the related molecular mechanism was investigated. Cross-linking by genipin increased the stability of the type II collagen scaffolds, but deformed the configuration of scaffolds and changed the intrinsic properties of type II collagen. scaffold cross-linked with 0.1% genipin improved the biostability on the basis of maintaining the configuration of scaffold. In addition, the 0.1% genipin-cross-linked scaffold promoted ADSCs proliferation and differentiation into NP-like cells, along with the increasing gene and protein expressions of Sonic Hedgehog (Shh). All these results suggested that 0.1% genipin was the optimal concentration to establish a bio-stable 3D type II collagen scaffold, which inducing ADSC proliferation and differentiation toward a NP-like phenotype through the activation of Shh signaling pathway. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1258-1268, 2018.

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“…This calcification occurs partially due to the use of chemical cross-linkers like glutaraldehyde, which can have toxic effects. However, the use of natural cross-linking agents including genipin and procyanides has been shown to inhibit calcification of vascular grafts [65]. The creation of larger-scale vascular grafts using ECM has proven the potential of ECM as a material for the creation of vascular channels.…”

Section: In Vitro Control Systems To Evaluate Cell-cecm Interplay At mentioning

confidence: 99%

Cardiac Extracellular Matrix Modification as a Therapeutic Approach

Hall

Ogle

2018

Advances in Experimental Medicine and Biology

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The cardiac extracellular matrix (cECM) is comprised of proteins and polysaccharides secreted by cardiac cell types, which provide structural and biochemical support to cardiovascular tissue. The roles of cECM proteins and the associated family of cell surface receptor, integrins, have been explored in vivo via the generation of knockout experimental animal models. However, the complexity of tissues makes it difficult to isolate the effects of individual cECM proteins on a particular cell process or disease state. The desire to further dissect the role of cECM has led to the development of a variety of in vitro model systems, which are now being used not only for basic studies but also for testing drug efficacy and toxicity and for generating therapeutic scaffolds. These systems began with 2D coatings of cECM derived from tissue and have developed to include recombinant ECM proteins, ECM fragments, and ECM mimics. Most recently 3D model systems have emerged, made possible by several developing technologies including, and most notably, 3D bioprinting. This chapter will attempt to track the evolution of our understanding of the relationship between cECM and cell behavior from in vivo model to in vitro control systems. We end the chapter with a summary of how basic studies such as these have informed the use of cECM as a direct therapy.

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Crosslinking strategies for preparation of extracellular matrix-derived cardiovascular scaffolds

Cited by 158 publications

References 74 publications

Folic acid-targeted disulfide-based cross-linking micelle for enhanced drug encapsulation stability and site-specific drug delivery against tumors

Folic acid-targeted disulfide-based cross-linking micelle for enhanced drug encapsulation stability and site-specific drug delivery against tumors

Genipin‐cross‐linked type II collagen scaffold promotes the differentiation of adipose‐derived stem cells into nucleus pulposus‐like cells

Cardiac Extracellular Matrix Modification as a Therapeutic Approach

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