Crosstalk between Methylation and ncRNAs in Breast Cancer: Therapeutic and Diagnostic Implications

Liu, Yitong; Leng, Ping; Liu, Yan; Guo, Jiao; Zhou, Hao

doi:10.3390/ijms232415759

Cited by 13 publications

(6 citation statements)

References 138 publications

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“…For example, in cervical cancer, m6A methylation was shown to alter circRNA modifications, thereby increasing the risk of cervical cancer metastasis 9 . Liu et al [ 34 ] discovered that methylation of noncoding RNAs due to m6A methylation in breast cancer can be utilized to predict relevant therapeutic drug targets and target sites for RNA interactions. Although the regulatory influence of N6-methyladenosine (m6A) on RNA has been extensively documented, microRNAs (miRNAs) offer an additional layer of post-transcriptional control.…”

Section: Discussionmentioning

confidence: 99%

YTHDF2-Mediated m6A methylation inhibition by miR27a as a protective mechanism against hormonal osteonecrosis in BMSCs

Yuan,

Liu,

Abudoukadier

et al. 2024

BMC Musculoskelet Disord

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Background With the increasing incidence of steroid-induced necrosis of the femoral head (SNFH), numerous scholars have investigated its pathogenesis. Current evidence suggests that the imbalance between lipogenesis and osteoblast differentiation in bone marrow mesenchymal stem cells (BMSCs) is a key pathological feature of SNFH. MicroRNAs (miRNAs) have strong gene regulatory effects and can influence the direction of cell differentiation. N6-methyladenosine (m6A) is a prevalent epigenetic modification involved in diverse pathophysiological processes. However, knowledge of how miRNAs regulate m6A-related factors that affect BMSC differentiation is limited. Objective We aimed to investigate the role of miR27a in regulating the expression of YTHDF2 in BMSCs. Methods We compared miR27a, YTHDF2, and total m6A mRNA levels in SNFH-affected and control BMSCs. CCK-8 and TUNEL assays were used to assess BMSC proliferation and apoptosis. Western blotting and qRT‒PCR were used to measure the expression of osteogenic (ALP, RUNX2, and OCN) and lipogenic (PPARγ and C/EBPα) markers. Alizarin Red and Oil Red O staining were used to quantify osteogenic and lipogenic differentiation, respectively. miR27a was knocked down or overexpressed to evaluate its impact on BMSC differentiation and its relationship with YTHDF2. Bioinformatics analyses identified YTHDF2 as a differentially expressed gene in SNFH (ROC analysis) and revealed potential signaling pathways through GSEA. The effects of YTHDF2 silencing on the lipogenic and osteogenic functions of BMSCs were assessed. Results miR27a downregulation and YTHDF2 upregulation were observed in the SNFH BMSCs. miR27a knockdown/overexpression modulated YTHDF2 expression, impacting BMSC differentiation. miR27a silencing decreased m6A methylation and promoted osteogenic differentiation, while YTHDF2 silencing exerted similar effects. GSEA suggested potential signaling pathways associated with YTHDF2 in SNFH. Conclusion miR27a regulates BMSC differentiation through YTHDF2, affecting m6A methylation and promoting osteogenesis. This finding suggests a potential therapeutic target for SNFH.

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Section: Discussionmentioning

confidence: 99%

YTHDF2-Mediated m6A methylation inhibition by miR27a as a protective mechanism against hormonal osteonecrosis in BMSCs

Yuan,

Liu,

Abudoukadier

et al. 2024

BMC Musculoskelet Disord

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“…With the in-depth exploration of ncRNAs, mounting evidence suggests that ncRNAs play a substantial role in BC biological behaviors through diverse mechanisms, including acting as miRNA sponges or decoys, interacting with RNA-binding proteins (RBPs), translating into functional peptides, and undergoing epigenetic modifications. [23][24][25] Nevertheless, the mechanisms and biological functions of ncRNAs reliant on m6A methylation in BC remain incompletely understood (Figure 1, Table 1).…”

Section: Regulation Of M6a By Ncrnas In Bcmentioning

confidence: 99%

Unraveling the cross‐talk between N6‐methyladenosine modification and non‐coding RNAs in breast cancer: Mechanisms and clinical implications

Liu,

Xie,

Sui

et al. 2024

Intl Journal of Cancer

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In recent years, breast cancer (BC) has surpassed lung cancer as the most common malignant tumor worldwide and remains the leading cause of cancer death in women. The etiology of BC usually involves dysregulation of epigenetic mechanisms and aberrant expression of certain non‐coding RNAs (ncRNAs). N6‐methyladenosine (m6A), the most prevalent RNA modification in eukaryotes, widely exists in ncRNAs to affect its biosynthesis and function, and is an important regulator of tumor‐related signaling pathways. Interestingly, ncRNAs can also regulate or target m6A modification, playing a key role in cancer progression. However, the m6A‐ncRNAs regulatory network in BC has not been fully elucidated, especially the regulation of m6A modification by ncRNAs. Therefore, in this review, we comprehensively summarize the interaction mechanisms and biological significance of m6A modifications and ncRNAs in BC. Meanwhile, we also focused on the clinical application value of m6A modification in BC diagnosis and prognosis, intending to explore new biomarkers and potential therapeutic targets.

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“… 2021 , Liu et al . 2022 ). Proteins and protein complexes that epigenetically modify DNA and histones were classified into epigenetic writers, readers , and erasers (Biswas and Rao 2018 ).…”

Section: Brief Description Of Epigenetic Changesmentioning

confidence: 99%

Epigenetic effects of short-chain fatty acids from the large intestine on host cells

Stein

Riber

2023

microLife

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Adult humans harbor at least as many microbial cells as eukaryotic ones. The largest compartment of this diverse microbial population, the gut microbiota, encompasses the collection of bacteria, archaea, viruses, and eukaryotic organisms that populate the gastrointestinal tract, and represents a complex and dynamic ecosystem that has been increasingly implicated in health and disease. The gut microbiota carries ∼100-to-150-times more genes than the human genome and is intimately involved in development, homeostasis, and disease. Of the several microbial metabolites that have been studied, short-chain fatty acids emerge as a group of molecules that shape gene expression in several types of eukaryotic cells by multiple mechanisms, which include changing histone acetylation, inducing DNA methylation, or through microRNA-mediated signaling. Butyric acid, one of the most extensively studied short-chain fatty acids, reaches higher concentrations in the colonic lumen, where it can be used as fuel by healthy colonocytes, than in the colonic crypts, where stem cells reside. In colon cancer cells, which preferentially metabolize glucose by glycolysis, butyric acid induces epigenetic modifications, leading to cell cycle progression inhibition and apoptosis. The lower butyrate concentration in the colonic crypts epigenetically inhibits negative regulators of the cell cycle, pointing towards the importance of the crypts in providing a protective niche for stem cells. A better understanding of the interface between the gut microbiota metabolites and epigenetic changes in eukaryotic cells promises to unravel in more detail processes that occur physiologically and, as part of disease, help develop novel biomarkers, and identify new therapeutic modalities.

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Crosstalk between Methylation and ncRNAs in Breast Cancer: Therapeutic and Diagnostic Implications

Cited by 13 publications

References 138 publications

YTHDF2-Mediated m6A methylation inhibition by miR27a as a protective mechanism against hormonal osteonecrosis in BMSCs

YTHDF2-Mediated m6A methylation inhibition by miR27a as a protective mechanism against hormonal osteonecrosis in BMSCs

Unraveling the cross‐talk between N6‐methyladenosine modification and non‐coding RNAs in breast cancer: Mechanisms and clinical implications

Epigenetic effects of short-chain fatty acids from the large intestine on host cells

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