Crosstalk between the Purinergic and Immune Systems: Implications for the Glutathione Antioxidant System in Health and Disease

Assmann, Charles Elias; Stefanello, Naiara; Bottari, Nathieli B.; Baldissarelli, Jucimara; Schetinger, Maria Rosa Chitolina; Morsch, Vera Maria; Bagatini, Margarete Dulce

doi:10.5772/intechopen.92881

Cited by 2 publications

(2 citation statements)

References 134 publications

(177 reference statements)

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“…Glutathione is an antioxidant and has been linked to the development and progression of several diseases, such as cancer, rheumatoid arthritis, insulin-dependent diabetes mellitus, and multiple sclerosis [29]. The pyrimidine signaling and glutathione networks are closely related and regulate inflammatory processes [30]. These findings further indicate a role of pyrimidine signaling in conditions causing damage to the salivary glands.…”

Section: Discussionmentioning

confidence: 95%

Saliva Metabolomics in Dry Mouth Patients with Head and Neck Cancer or Sjögren’s Syndrome

Hynne

Sandås

Elgstøen

et al. 2022

Cells

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The etiology of dry mouth conditions is multi-faceted. Patients radiated after head and neck cancer (HNC) and those with primary Sjögren’s syndrome (pSS) share many of the same symptoms despite different causes. With the aim of better understanding the pathophysiology and biochemical processes behind dry mouth with different etiologies, we investigated the metabolic profile of 10 HNC patients, 9 pSS patients and 10 healthy controls using high-performance liquid chromatography-high resolution mass spectrometry (HPLC-MS) metabolomics. Principal component analysis (PCA) revealed different metabolic profiles when comparing all subjects included in the study. Both patient groups showed higher ratios of several pyrimidine nucleotides and nucleosides when compared to controls. This finding may indicate that purinergic signaling plays a role in dry mouth conditions. Moreover, significantly increased levels of DL-3-aminoisobutyric acid were found in HNC patients when compared to controls, and a similar tendency was observed in the pSS patients. Furthermore, a dysregulation in amino acid metabolism was observed in both patient groups. In conclusion, metabolomics analysis showed separate metabolic profiles for HNC and pSS patients as compared to controls that could be useful in diagnostics and for elucidating the different pathophysiologies. The demonstrated dysregulation of pyrimidine nucleotides and levels of metabolites derived from amino acids in the patient groups should be studied further.

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Section: Discussionmentioning

confidence: 95%

Saliva Metabolomics in Dry Mouth Patients with Head and Neck Cancer or Sjögren’s Syndrome

Hynne

Sandås

Elgstøen

et al. 2022

Cells

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“…AMP is hydrolyzed by ecto-5nucleotidase/CD73 to adenosine. Adenosine is mainly recognized as an immunosuppressive nucleotide and is targeted for immune treatments [8]. Finally, adenosine is converted to inosine by adenosine deaminase (ADA) [9].…”

Section: Introductionmentioning

confidence: 99%

Curcumin and Vinblastine Disturb Ectonucleotides Enzymes Activity and Promote ROS Production in Human Cutaneous Melanoma Cells

Lunkes

Palma

Assmann

et al. 2022

Braz. arch. biol. technol.

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Cancer is the leading cause of death. Melanoma skin cancer originates in melanocytes and represents 80% of the deaths associated with skin cancer. Vinblastine (VIN) is a chemotherapeutic agent used in the treatment of cancer through disrupting mitotic spindle and tumor development. Curcumin (CUR), a compound extracted from the rhizomes of the Curcuma longa plant, has beneficial effects in preventing the development and progression of cancer while modulating the immune response and oxidative stress. The expression of purinergic receptors, ecto-enzymes, and adenosine can modulate the inflammatory responses in cancer. The activity of enzymes, the markers of cell damage in oxidative stress, the generation of reactive oxygen species (ROS), and the activities of ecto-enzymes in the melanoma cell line were investigated. The human melanoma cell line was treated with curcumin (40 µM), vinblastine (VIN) (20 nM), or a combination of both for 24h. Oxidative stress enzymes and byproducts were measured and compared against the activity of ecto-enzymes. There was a marked increase in ROS production in all groups, but an increase in protein carbonylation was only detected in the VIN group. CUR had an inhibitory effect on extracellular ADP HIGHLIGHTS• Melanoma is a cancer with poor prognosis, and metastasis.• Curcumin is a compound with many beneficial effects regarding prevention and adjuvant treatment of cancer • Vinblastine and curcumin greatly promoted production of ROS in melanoma cells• Curcumin disrupted adenosine formation cascade, reducing imunosupression at tumoral site.

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Crosstalk between the Purinergic and Immune Systems: Implications for the Glutathione Antioxidant System in Health and Disease

Cited by 2 publications

References 134 publications

Saliva Metabolomics in Dry Mouth Patients with Head and Neck Cancer or Sjögren’s Syndrome

Saliva Metabolomics in Dry Mouth Patients with Head and Neck Cancer or Sjögren’s Syndrome

Curcumin and Vinblastine Disturb Ectonucleotides Enzymes Activity and Promote ROS Production in Human Cutaneous Melanoma Cells

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