Saliva Metabolomics in Dry Mouth Patients with Head and Neck Cancer or Sjögren’s Syndrome

Abstract

The etiology of dry mouth conditions is multi-faceted. Patients radiated after head and neck cancer (HNC) and those with primary Sjögren’s syndrome (pSS) share many of the same symptoms despite different causes. With the aim of better understanding the pathophysiology and biochemical processes behind dry mouth with different etiologies, we investigated the metabolic profile of 10 HNC patients, 9 pSS patients and 10 healthy controls using high-performance liquid chromatography-high resolution mass spectrometry (HPLC-MS) metabolomics. Principal component analysis (PCA) revealed different metabolic profiles when comparing all subjects included in the study. Both patient groups showed higher ratios of several pyrimidine nucleotides and nucleosides when compared to controls. This finding may indicate that purinergic signaling plays a role in dry mouth conditions. Moreover, significantly increased levels of DL-3-aminoisobutyric acid were found in HNC patients when compared to controls, and a similar tendency was observed in the pSS patients. Furthermore, a dysregulation in amino acid metabolism was observed in both patient groups. In conclusion, metabolomics analysis showed separate metabolic profiles for HNC and pSS patients as compared to controls that could be useful in diagnostics and for elucidating the different pathophysiologies. The demonstrated dysregulation of pyrimidine nucleotides and levels of metabolites derived from amino acids in the patient groups should be studied further.

Effects of medwakh smoking on salivary metabolomics and its association with altered oral redox homeostasis among youth

“…The untargeted salivary metabolite profiling revealed evidence of perturbation in nucleotide metabolites in medwakh smokers. Elevated levels of pyrimidine nucleotides and nucleosides in medwakh smokers point to changes in the purinergic signaling, which have been linked to the pathophysiology of salivary hypofunction 29 , a condition that often result in dry mouth or xerostomia.…”

Saliva Metabolomic Profile in Dental Medicine Research: A Narrative Review

“…The complete set of small molecular metabolites is called the “metabolome” [ 17 ]. “Metabolomics” is defined as the latest of the –omics technologies, investigating metabolites within biofluids, cells and tissues [ 18 ].…”

Genômica funcional: engenharia reversa por meio de métodos de altaperformance na saliva e sangue de pacientes com síndrome de Sjögren

“…Esses quatro compostos foram relatados como aumentados em todos os tempos estudados quando comparados ao nível estabelecido como linha basal do grupo controle. Hynne et al (2022b), compararam, por meio de cromatografia líquida acoplada a espectrometria de massa, a saliva total não estimulada de pacientes com SS, indivíduos com neoplasia de cabeça e pescoço e indivíduos saudáveis, encontrando diferença significante de 17 metabólitos aumentados na SS e 3 no grupo controle saudável.…”

“…Quando comparados os grupos de pacientes com neoplasia de cabeça e pescoço e SS, 15 compostos se revelaram diferencialmente aumentados na SS e 3 no grupo de neoplasias (Hynne et al, 2022b). Bellocchi et al (2019) Fonte: a autora.…”

“…O estudo de Kageyama et al (2015), demonstrou 83 metabólitos diferencialmente abundantes na saliva dos pacientes com SS, entre eles o 4-Hidroxibutirato (↓) e a hipoxantina (↑), ambos apresentaram o mesmo padrão de abundância encontrados em nossas análises. Não foram encontrados metabólitos em comum com os outros dois estudos que utilizaram saliva para a análise do metaboloma em pacientes com SS (Herrala et al, 2021;Hynne et al, 2022b).…”