Crude extract of Trichoderma elicits agarwood substances in cell suspensionculture of the tropical tree, Aquilaria malaccensis Lam.

Jayaraman, Shashita; Mohamed, Rozi

doi:10.3906/tar-1404-63

Cited by 24 publications

(21 citation statements)

References 28 publications

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“…24 Wood samples (10 g) were extracted with methanol (150 mL) for 6 h. Then, the methanol solvent was evaporated and dried extract samples were kept at -18 °C until further experiments. [26][27][28][29] Tab 1. Hence this solvent is used favorably to extract polar components including phenolic compounds and flavonoids.…”

Section: Extractionmentioning

confidence: 99%

DNA protection, antioxidant, antibacterial and enzyme inhibition activities of heartwood and sapwood extracts from juniper and olive woods

et al. 2015

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In this study, DNA protective, antioxidant, antibacterial and enzyme inhibiting properties of methanol extracts obtained from juniper and olive heartwood and sapwood were determined. These extracts were tested by five antioxidant methods (DPPH scavenging, FRAP, CUPRAC, metal chelating and phosphomolybdenum). Generally, heartwood extracts of both species are more efficient for DPPH radical scavenging activity, cupric ion reducing activity, ferric reducing antioxidant power and metal chelating activity than sapwood extracts. When compared to heartwood extracts, sapwood extracts have larger inhibition zone in disk diffusion test. In addition, all extracts showed high antibacterial activity against Staphylococcus aureus. DNA protection of both extracts had a capacity to inhibit the DNA damage arisen from Fenton's reagent. The highest DNA protective activity was observed in juniper sapwood extract with 84%. Furthermore, other extracts also indicated more than 60% of DNA protective activity. Olive wood extracts displayed the strongest enzyme inhibition activities against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). Although juniper heartwood extracts showed highest anti-amylase, anti-glucosidase and anti-tyrosinase effects, they had no ability for inhibition BChE.The methanol extracts of olive samples demonstrated the most antioxidant activity (DPPH, CUPRAC and FRAP). In addition, juniper samples showed the highest anti-amylase, anti-tyrosinase, metal chelating and DNA protective activity. According to these results, the extracts of juniper and olive wood can be considered as a source of natural bio active agents for dietary, pharmacological and medicinal applications. This research will also serve as a base for future studies about biological activities of wood extracts.

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Section: Extractionmentioning

confidence: 99%

DNA protection, antioxidant, antibacterial and enzyme inhibition activities of heartwood and sapwood extracts from juniper and olive woods

et al. 2015

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“…Meanwhile, Study 3 used methanol to extract dry cell of agarwood that harvested from tissue culture. Jayaraman and Mohamed (2015) were developed plant tissue culture from agarwood leaves cell for rapid mass production to regenerate agarwood plant and produce secondary metabolites from the plant such as aromatic compounds. Suzuki et al (2017) extracted powdered Aquilaria filaria heartwood with 95% methanol (room temperature, 72 hr).…”

Section: Methanolmentioning

confidence: 99%

“…Interestingly, cell culture technique has been used by Study 3 to produce the aroma of agarwood in the laboratory. Jayaraman and Mohamed (2015) were established a cell suspension culture of Aquilaria malaccensis from leaf-derived callus and used a fungal elicitor to induce agarwood production in the culture. Cell suspension cultures were treated with Trichoderma extract; a fungal elicitor to increase fragrance intensity comparable to the aroma of agarwood.…”

Section: Methanolmentioning

confidence: 99%

Solvent used in extraction process of agarwood: a systematic review

Zakaria¹,

Talip²,

Kahar³

et al. 2020

Food Res.

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The method of extraction of Agarwood is usually dependent on the purpose of the extract. This systematic review aims to look at the number of studies that use different types of solvents in the process of extraction. There are 3 types of solvents commonly used in the extraction of agarwood essential oils namely methanol, ethanol and water. Each solvent produces different extracts in terms of quantity and quality of the final product. However, there is controversy over the use of solvents as it may cause a cytotoxic effect on the user. Another common type of solvent is water. Although water is a cheap solvent and relatively safe, aqueous extracts have more impurities that make isolating the desired compound difficult. After the extraction process, the crude extract was fractionated into the desired compounds and this technique is widely applied, especially in the whole process of extraction of the agarwood. In conclusion, extraction solvents have certain advantages and disadvantages, and this is one of the reasons why this extraction method depends on the purpose of using the final product such as agarwood essential oil.

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“…Menurut Jayaraman and Mohamed (2015), pada kombinasi 8 g/L inokulum kalus pada suspensi A. malaccensis dan 8 mg/L ekstrak Trichoderma terdeteksi beberapa senyawa penting gaharu seperti 8 -epi-gama-eudesmol, -guaiena, dan aloaromadendrena oksida-1.…”

Section: Induksi Biak Kalus Dan Biak Suspensi Sel Aquilaria Malaccensunclassified

INDUKSI BIAK KALUS DAN BIAK SUSPENSI SEL Aquilaria malaccensis Lam.

Leksonowati¹,

Witjaksono²,

Ratnadewi³

2017

BERITABIOLOGI

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Aquilaria malaccensis Lam. is a plant species producing fragrant woody material that contains some resin. The compounds can be used as medicine and perfume. Sesquiterpenoid, one group of compounds has been found being synthesized and subsequently extracted from callus and cell suspension culture of Aquilaria species. The aim of this research was to find a method of producing friable calli and cell suspension cultures from leaves or internodes of A. malaccensis in vitro by using suitable plant growth regulators; cell suspension that will suitably serve as material to produce sesquiterpenoid afterwards. Calli were established in almost all treatments of auxin-cytokinin on both leaves and internod explants. The treatment of 10 mg/L IBA induced the highest percentage of callus coverage from leaves with a rather compact structure. The combined treatment of 1–2 mg/L 2.4-D and 0.2–0.3 mg/L BA induced friable callus formation in more than 80% of cultures with 27–32% callus coverage percentage. The use of 2,4-D induced a better formation of cell suspension than Picloram, with maximum volume up to 7 mL. Cell suspension culture with fine and homogenous aggregate could be established in the medium supplemented with 0.5 –1 mg/L 2,4-D.

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Crude extract of Trichoderma elicits agarwood substances in cell suspensionculture of the tropical tree, Aquilaria malaccensis Lam.

Cited by 24 publications

References 28 publications

DNA protection, antioxidant, antibacterial and enzyme inhibition activities of heartwood and sapwood extracts from juniper and olive woods

DNA protection, antioxidant, antibacterial and enzyme inhibition activities of heartwood and sapwood extracts from juniper and olive woods

Solvent used in extraction process of agarwood: a systematic review

INDUKSI BIAK KALUS DAN BIAK SUSPENSI SEL Aquilaria malaccensis Lam.

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