Crude Oil Degradation by a Novel Strain Pseudomonas aeruginosa AQNU-1 Isolated from an Oil-Contaminated Lake Wetland

Abstract

In this study, a novel crude oil degrading bacterium was isolated from an oil-contaminated freshwater lake using crude oil as the sole carbon source. The strain was named Pseudomonas aeruginosa AQNU-1 based on the analyses of its morphological characteristics and 16S rRNA gene sequencing. Gas chromatography-mass spectrometry (GC-MS) was carried out to investigate the degradation of crude oil fractions under dynamic (37 °C, 180 r/min) and static (37 °C, 0 r/min) cultivation over three months of continuous enrichment in the laboratory. It was found that strain AQNU-1 exhibited stronger biodegradation efficiency for n-alkanes of C13–C35 under dynamic cultivation with degradation ratios of 87–100% compared to ratios of 74–100% under static cultivation. Furthermore, this strain could fully utilize alkylcyclohexane (M/Z 82), alkylbenzene (M/Z 92) and alkyltoluene (M/Z 106) in crude oil under both conditions. It also had better biodegradability of partial aromatic compounds in the crude oil, showing differences within compound families of aromatic hydrocarbons. Further, the potential degradation ability of this isolated strain decreased with increasing molecular weight, with the dynamic condition performing better in general. These results suggest that the isolated strain has great potential to assimilate indigestible crude-oil contaminants under different hydrological conditions, providing a valuable microbiological resource for in situ remediation of natural wetlands.

Exploiting bacterial isolates for diesel degrading potential under in vitro conditions

“…Additionally, in the current experiment indicate the presence of various functional groups and bond strengths in the samples subjected to analysis, another study reported that the diesel oil act as a medium distillate of petroleum possessing different proportions of branched alkanes, nalkanes, olefins with low concentration of aromatic polycyclic components (13,33). According to the analysis, the absence of characteristic peaks in the region of 1610-2042 in extracted samples indicated absence of nitro compound linkage.…”

Bacterial Communities Associated with Crude Oil Bioremediation through Composting Approaches with Indigenous Bacterial Isolate

“…Pseudomonas is one of the well-known hydrocarbon degraders using the terminal oxidation pathway [31]. Recently, a member of this genus, P. aeruginosa, was isolated from an oil-contaminated lake wetland and reported to degrade up to 100% of C13-C35 hydrocarbons in crude oil [32]. In another work, this species was reported to degrade n-alkanes and polycyclic aromatic hydrocarbons at efficiencies of 80% and 98%, respectively [31].…”

Succession Patterns of Microbial Composition and Activity following the Diesel Spill in an Urban River

“…Pseudomonas sp. has been observed as a main oil hydrocarbon responder in fresh water from lake wetlands [ 55 ] and rivers [ 56 ]. At day 7, the relative abundance of these genera slightly decreases and they are outperformed by Aquabacterium , which is the most abundant genus.…”