Crustacean Brain Lipofuscin: An Examination of the Morphological Pigment in the Fresh-Water Crayfish Cherax cuspidatus (Parastacidae)

Sheehy, M. R. J.

doi:10.2307/1548562

Cited by 43 publications

(41 citation statements)

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“…Nelson and Morton, 1979;Moore et al, 1980;Mathew and Damodaran, 1997), crustaceans (e.g. Sheehy, 1989;Wahle et al, 1996;Medina et al, 2000;Bluhm et al, 2001a,b) and vertebrates (Eldred et al, 1982). This supports the view that the connective tissue granules in E. exalbida are lipofuscin.…”

Section: Is the Substance Found In E Exalbida Lipofuscin?mentioning

confidence: 66%

“…Donato and Sohal, 1978;Moore et al, 1980;Bassin et al, 1982; 0022-0981/02/$ -see front matter D 2002 Elsevier Science B.V. All rights reserved. PII: S 0 0 2 2 -0 9 8 1 ( 0 2 ) 0 0 2 4 0 -X Sheehy, 1989;Mathew and Damodaran, 1997;Sheehy et al, 1998;Bluhm et al, 2001a). The rate of lipofuscin formation is assumed to depend mainly on cellular oxygen consumption (Sohal, 1981;Zs-Nagy, 1988;Katz et al, 1984).…”

Section: Introductionmentioning

confidence: 99%

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Survivorship of juvenile surf clams Donax serra (Bivalvia, Donacidae) exposed to severe hypoxia and hydrogen sulphide

Laudien

Schiedek

Brey

et al. 2002

Journal of Experimental Marine Biology and Ecology

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In the hard clam Eurhomalea exalbida, autofluorescent granules were detected in high concentrations in the connective tissue around the intestine and in other tissues. Autofluorescence combined with Sudan black B and PAS positive reactions suggested that these granules were lipofuscin-like. The concentration of this material in the connective tissue (CT) around the intestine was quantified by image analysis and expressed as total area fraction occupied by lipofuscin granules. Lipofuscin concentration was distinctly better related with individual age as determined from stable isotope-validated shell growth bands than with any morphometric parameter. This relationship was described best by a Gompertz model: Lipofuscin t = 24.79e À e À 0.029(Age t À 58.578) (N = 38; r nl 2 = 0.882). Age was predicted from lipofuscin CT concentration by a von Bertalanffy model: Age t = 68.00 (1 À e À 0.146(Lipofuscin t + 0.028) ) 0.664 (N = 38; r nl 2 = 0.933). Our findings suggest that lipofuscin CT concentration in E. exalbida is a function of individual age. If this holds true for bivalves in general, lipofuscin may be a suitable proxy for age in species with less clear shell growth band patterns. D

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Section: Is the Substance Found In E Exalbida Lipofuscin?mentioning

confidence: 66%

Section: Introductionmentioning

confidence: 99%

Survivorship of juvenile surf clams Donax serra (Bivalvia, Donacidae) exposed to severe hypoxia and hydrogen sulphide

Laudien

Schiedek

Brey

et al. 2002

Journal of Experimental Marine Biology and Ecology

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“…Once in the laboratory, they were removed from the fixative solution, washed in running water, weighed (whole body weight, in g), and the cephalothorax was separated from the abdomen. The brain was removed under a stereomicroscope, leaving the circum-esophagus commissura longer than the optical nerves, in order to allow the proper orientation of the piece during the inclusion (SHEEHY, 1989;ENCARNACÃO & CASTRO, 2001). The sample was thereafter embedded in Bio Plast-extra paraffin.…”

Section: Materials and ªEthodsmentioning

confidence: 99%

Evidence of lipofuscin accumulation in the deep-water red shrimp Aristaeomorpha foliacea (Risso, 1827)

Mezzasalma¹,

Zagra²,

Stefano³

et al. 2008

Medit. Mar. Sci.

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Lipofuscin, a non-degradable, degenerative fluorescent pigment which accumulates in post-mitotic cells, represents a promising method for ageing marine crustaceans. The presence and accumulation of lipofuscin has been studied in the deep-water red shrimp Aristaeomorpha foliacea (Risso, 1827) to assess its use as a tool for ageing larger (i.e., older) specimens and thus improve knowledge of the growth and longevity of this species. Specimens, gathered during experimental trawl surveys carried out in the Strait of Sicily (Mediterranean Sea), were stored directly on-board in 10% buffered formaldehyde solution; their brain was thereafter removed, prepared with various current histological techniques and examined with a binocular microscope. Thin sections of the olfactory lobe cell mass were also analyzed using fluorescence microscopy, and the lipofuscin concentration was measured through image analysis. Various indices were computed for each individual by pooling data from many images: number and coverage of the lipofuscin granules per unit area, and mean individual area of the granules. Lipofuscin was detected in all specimens investigated with characteristics (grain typology and dimension) strictly resembling those already described in other crustacean species. The present preliminary results encourage further studies to develop and validate a methodology based on the use of lipofuscin for improving the relative ageing of large A. foliacea shrimps.

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“…Brains of the investigated species were prepared for lipofuscin measurement essentially as described for various decapod species by Sheehy (1989Sheehy ( , 1990a and Sheehy and Wickins (1994). The brains were dissected and dehydrated in ascending ethanol concentrations from 70% to 100%.…”

Section: Sample Preparationmentioning

confidence: 99%

“…In decapods, the (posterior) lateral somacluster of the olfactory lobe was localised (nomenclature after Sandeman et al 1992); this is composed of cell bodies of ascending olfactory projection neurons (Schmidt and Harzsch 1999). Lipofuscin has proven to be especially dense in this area in decapod crustaceans (Sheehy 1989(Sheehy , 1990bSheehy et al 1995Sheehy et al , 1998. In amphipods, we identi®ed regions of high lipofuscin density, so far not described in the literature, and afterwards used them for analysis.…”

Section: Fluorescence Microscopymentioning

confidence: 99%

Occurrence of the autofluorescent pigment, lipofuscin, in polar crustaceans and its potential as an age marker

et al. 2001

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In crustaceans, the lack of reliable methods often prevents the determination of individual age. The quanti®cation of the auto¯uorescent age pigment, lipofuscin, has revealed promising results in boreal and tropical species. We studied the presence of morphological lipofuscin and its possible application as an age marker in ®ve Arctic and ®ve Antarctic species, comprising decapods, amphipods and a euphausiid. Lipofuscin granules were located in the brain, using confocal¯uorescence microscopy, and quanti®ed from digital images. The pigment was found in 94 of 100 individuals and in all 10 species, and granules occurred in easily detectable amounts in 5 species. Two scavenging amphipod species, the Antarctic Waldeckia obesa and the Arctic Eurythenes gryllus, revealed the most conspicuous and numerous granules. There was a broad, though weak, correlation of lipofuscin concentration with individual body size within a species, but not with absolute body size of one species compared to another. In larvae of the decapod Chorismus antarcticus, lipofuscin accumulation was quanti®ed over the 1st 4 months after larval release. Morphological lipofuscin is a potential index of age in those investigated species with a su cient accumulation rate of the pigment.

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Crustacean Brain Lipofuscin: An Examination of the Morphological Pigment in the Fresh-Water Crayfish Cherax cuspidatus (Parastacidae)

Cited by 43 publications

References 0 publications

Survivorship of juvenile surf clams Donax serra (Bivalvia, Donacidae) exposed to severe hypoxia and hydrogen sulphide

Survivorship of juvenile surf clams Donax serra (Bivalvia, Donacidae) exposed to severe hypoxia and hydrogen sulphide

Evidence of lipofuscin accumulation in the deep-water red shrimp Aristaeomorpha foliacea (Risso, 1827)

Occurrence of the autofluorescent pigment, lipofuscin, in polar crustaceans and its potential as an age marker

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