Cryo-preservation of Pollen for Hybrid Seed Production in Hot Pepper

Mathad, Rakesh C.; Vasudevan, S. N.; Patil, S. B.

doi:10.1007/978-81-322-0810-5_32

Cited by 5 publications

(5 citation statements)

References 1 publication

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“…It is also important to gain a better understanding of pollen viability and longevity and how it can be controlled. This will have positive outcomes for hybrid breeding, where increased pollen viability will improve cross-pollination efficiency and offer better options for pollen storage, e.g., cryogenic storage (Fritz and Lukaszewski, 1989; Mathad et al, 2013; Palupi et al, 2017).…”

Section: Conclusion: Da’s Role In Pollen Viability and Abiotic Stressmentioning

confidence: 99%

Pollen Developmental Arrest: Maintaining Pollen Fertility in a World With a Changing Climate

Pacini

Dolferus

2019

Front. Plant Sci.

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During evolution of land plants, the haploid gametophytic stage has been strongly reduced in size and the diploid sporophytic phase has become the dominant growth form. Both male and female gametophytes are parasitic to the sporophyte and reside in separate parts of the flower located either on the same plant or on different plants. For fertilization to occur, bi-cellular or tri-cellular male gametophytes (pollen grains) have to travel to the immobile female gametophyte in the ovary. To survive exposure to a hostile atmosphere, pollen grains are thought to enter a state of complete or partial developmental arrest (DA). DA in pollen is strongly associated with acquisition of desiccation tolerance (DT) to extend pollen viability during air travel, but occurrence of DA in pollen is both species-dependent and at the same time strongly dependent on the reigning environmental conditions at the time of dispersal. Several environmental stresses (heat, drought, cold, humidity) are known to affect pollen production and viability. Climate change is also posing a serious threat to plant reproductive behavior and crop productivity. It is therefore timely to gain a better understanding of how DA and pollen viability are controlled in plants and how pollen viability can be protected to secure crop yields in a changing environment. Here, we provide an overview of how DA and pollen viability are controlled and how the environment affects them. We make emphasis on what is known and areas where a deeper understanding is needed.

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Section: Conclusion: Da’s Role In Pollen Viability and Abiotic Stressmentioning

confidence: 99%

Pollen Developmental Arrest: Maintaining Pollen Fertility in a World With a Changing Climate

Pacini

Dolferus

2019

Front. Plant Sci.

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“…As previously demonstrated, the decrease in pollen germination can be attributed to the storage conditions and storage time [45,46]. Mathad et al [47] observed variable germination rates, fruit set, and seed development of cryo-preserved hot pepper pollens under different temperatures. Moreover, our results depicted significant differences between the two treatments used for lyophilization.…”

Section: Discussionmentioning

confidence: 89%

First Report of a Successful Development of Yam Hybrids (Dioscorea alata L.) from Lyophilized and Long-Term Stored Pollens

Mal閐on,

Nudol,

Perrot

et al. 2023

Phyton

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Various biological constraints including erratic and asynchronous flowering between male and female plants hinder successful hybrid development and genetic gains in greater yam breeding programs. Therefore, pollen storage has gained much attention to facilitate artificial pollinations and increase the genetic gains. This 4-year study aimed at developing a practical long-term pollen storage technique for the successful development of yam hybrids. Fresh pollens were collected from two Dioscorea alata males, then lyophilized (two lyophilization treatments were applied), followed by storage at room temperature (24°C-25°C) for 12 months. Moreover, the lyophilized and stored pollens were tested for viability by crossing with four female varieties. Our results showed that lyophilization is effective for achieving viable pollens after 12 months of storage. Treatment 1 (48 h drying) showed higher pollen germination and fertility rates than Treatment 2 (72 h drying). Although we observed a reduction in viability of lyophilized pollens after 12 months of storage, we generated hybrid seedlings with success rates from 12% to 21% compared to 21%-31% when using fresh pollens. Paternity testing based on molecular genotyping confirmed the hybrid status of the obtained seedlings, which grew well in a greenhouse. Lyophilization is a practical approach for a long-term storage of greater yam pollen samples. This protocol will positively impact yam breeding programs particularly in developing countries.

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“…As previously demonstrated, the decrease in pollen germination can be attributed to the storage conditions and storage time [45,46]. Mathad et al, [47] observed variable germination rates, fruit set, and seed development of cryo-preserved hot pepper pollens under different temperatures. Moreover, our results depicted significant differences between the two treatments used for lyophilization.…”

Section: Discussionmentioning

confidence: 95%

“…Mathad et al, [47] observed variable germination rates, fruit set, and seed development of cryo-preserved hot pepper pollens under different temperatures. Moreover, our results depicted significant differences between the two treatments used for lyophilization.…”

Section: Discussionmentioning

confidence: 99%

First report of a successful development of yam hybrids (Dioscorea alataL.) from lyophilized and long-term stored pollens

Maledon

Perrot

Gravillon

et al. 2023

Preprint

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Background: Greater yam, Dioscorea alata L., is a significant food security crop in tropical areas. However, low genetic diversity and various biological constraints, including susceptibility to viruses, ploidy, erratic and low flowering intensity, and asynchronous flowering hinder successful hybrid development and genetic gains in greater yam breeding programs. Therefore, pollen storage has gained much attention to facilitate genetic material exchanges, artificial pollinations and to increase the genetic gains in breeding programs. This 4-year study aimed at developing a practical long-term pollen storage technique for the successful development of yam hybrids. Fresh pollens were collected from two D. alata males, then lyophilized (two lyophilization treatments were applied), followed by storage at room temperature (24-25 oC) for 12 months. Moreover, the lyophilized and stored pollens were tested for viability by crossing with four female varieties. Results: Our results showed that lyophilization is effective for achieving viable pollens after 12 months of storage. Treatment 1 (48 h drying) showed higher pollen germination and fertility rates than Treatment 2 (72 h drying). Although we observed a reduction in viability of lyophilized pollens after 12 months of storage, we generated hybrid seedlings with success rates from 12 to 21% compared to 21-31% when using fresh pollens. Paternity testing based on molecular genotyping confirmed the hybrid status of the obtained seedlings, which grew well in a greenhouse. Conclusions: The results signify the importance of pollen lyophilization for yam breeding programs.

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Cryo-preservation of Pollen for Hybrid Seed Production in Hot Pepper

Cited by 5 publications

References 1 publication

Pollen Developmental Arrest: Maintaining Pollen Fertility in a World With a Changing Climate

Pollen Developmental Arrest: Maintaining Pollen Fertility in a World With a Changing Climate

First Report of a Successful Development of Yam Hybrids (Dioscorea alata L.) from Lyophilized and Long-Term Stored Pollens

First report of a successful development of yam hybrids (Dioscorea alataL.) from lyophilized and long-term stored pollens

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