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The present investigation was carried out to find faster chemical methods to determine genetic purity in ten pigeon pea varieties. The faster methods are Ferrous Sulphate test, Sodium hydroxide test, peroxidase test, seedling growth response to GA 3, kinetin and 2-4D. Histogram using pixel luminance in ferrous sulphate test based on the grey scale produces values ranging from 17377 to 90616 which can be used to distinguish pigeon pea varieties. Similarly, sodium hydroxide test produced six coloured palette based on RGB values can distinguish varieties in pigeon pea. Also in peroxidase test, values for all ten pigeon pea varieties based on absorbance (0.252-2.372), peroxidase enzyme activity (0.791-7.442 IU/mg) and peroxidase enzyme volume (1.581-14.883 U/g) can be used for distinguishing varieties. Among the seedling growth response tests, in GA 3 test two varieties Asha (-2) and GC-11-39 (-6) were not responded and were recorded lowest values than the control. But remaining varieties can be distinguished as medium to high response types. Also in kinetin test two varieties not responded (GRG-811 and Asha) and all the other varieties can be distinguished based on this test. In case of 2-4D test all the varieties were recorded as susceptible. The co-efficient of variation for all the three tests based on the increase in coleoptile length over the control was ranged from 0.20 (Kinetin test) to 0.37 (GA 3 test).

SUMMARY :Investigations were carried out to study the varietal characterization of paddy hybrid and its parents through chemical tests and using iamge analyzer. Among the different chemical tests, phenol and modified phenol test showed differences in which male seeds had no change in colour development whereas, seeds of female and hybrid showed brown colouration. In case of FeSO 4 , KOH and NaOH tests both parents and hybrid showed similar colour reaction. The results obtained through image analysis with regard to seed length and seed width could be useful for varietal characterization of paddy genotypes. Based on seed length both parents and hybrid were grouped as medium length, based on seed width, parents and hybrid were grouped as broad seeds. But based on Seed length/ width ratio of seeds, female and hybrid seeds were grouped as elongated seeds whereas, male as semi elongated.

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