Cryoenzymology in Aqueous Media

Douzou, Pierre

doi:10.1002/9780470122969.ch1

Cited by 10 publications

(2 citation statements)

References 57 publications

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“…Elaborated and systematic development of low-temperature methods for biochemistry by Pierre Douzou and his colleagues produced dozens seminal publications, summarized in many excellent reviews (24-26,35,84-87) and a book (20), which describe in detail theoretical basis and numerous indispensable details of experimental methods. The works of Roy Daniel and collaborators also provide a useful insight into the role of enzyme dynamics in the catalytic activity by using low-temperature methods and cryosolvents (27,28,88).…”

Section: Additional Readingmentioning

confidence: 99%

“…Despite the fact that some buffers, such as acetate and phosphate have much smaller pK a temperature dependence than amine buffers, a general recommendation for the most stable buffer system is difficult because of the changes of ionization enthalpies of these groups in organic and mixed solvents. Several reviews by Douzou provide tabulated reference data for several buffers in mixed aqueous – organic solvents (24, 29, 36). …”

Section: Introductionmentioning

confidence: 99%

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Cryoradiolysis and Cryospectroscopy for Studies of Heme-Oxygen Intermediates in Cytochromes P450

Denisov

Grinkova

Sligar

2012

Methods in Molecular Biology

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Cryogenic radiolytic reduction is one of the most simple and convenient methods of generation and stabilization of reactive iron-oxygen intermediates for mechanistic studies in chemistry and biochemistry. The method is based on one-electron reduction of the precursor complex in frozen solution via exposure to the ionizing radiation at cryogenic temperatures. Such approach allows for accumulation of the fleeting reactive complexes which otherwise could not be generated at sufficient amount for structural and mechanistic studies. Application of this method allowed for characterizing of peroxoferric and hydroperoxo-ferric intermediates, which are common for the oxygen activation mechanism in cytochromes P450, heme oxygenases and nitric oxide synthases, as well as for the peroxide metabolism by peroxidases and catalases.

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Section: Additional Readingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cryoradiolysis and Cryospectroscopy for Studies of Heme-Oxygen Intermediates in Cytochromes P450

Denisov

Grinkova

Sligar

2012

Methods in Molecular Biology

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The Rapid‐Flow‐Quench Method in the Study of Fast Reactions in Biochemistry: Extension to Subzero Conditions

Barman

Travers

1985

Methods of Biochemical Analysis

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Anomalien des flüssigen Wassers

Lang

Lüdemann

1982

Angewandte Chemie

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Bei tiefen Temperaturen (T< 300 K) zeigt flussiges Wasser viele ungewohnliche physikalisch-chemische Eigenschaften, die durch die starke Direktivitat der Wasserstoffbriickenbindung und die damit verbundene energetische Bevorzugung einer tetraedrischen Nahordnung rnit schlechter Raumausfiillung verursacht werden. Diese Anomalien werden im metastabilen Bereich unterhalb der Schmelzdruckkurve wesentlich ausgepragter. Im vorliegenden Beitrag werden die Experimentalbefunde anhand der neueren Theorien diskutiert und rnit den Ergebnissen von Computer-Simulationen am kalten Wasser verglichen. Die Anomalien des Wassers, die sich z. B. im Dichtemaximum der flussigen Phase oder in der Abnahme der Viskositat rnit dem Druck manifestieren, sind auf den Bereich T<300K und p 5 2 0 0 MPa beschrankt. Bei diesen Drucken zeigt das unterkuhlte Wasser mit fallender Temperatur ein ungewohnliches Verhalten: Alle isobaren Eigenschaften deuten auf einen Phasenubergang bei T = ( TK -50 K), dessen physikalische Natur noch nicht eindeutig beschrieben werden kann. Dieses Phanomen ist bis jetzt ausschliefllich im flussigen Wasser beobachtet worden. -Kunftig wird man unterkuhlte wlBrige Losungen fur kinetische Untersuchungen verwenden und dadurch den zuganglichen dynamischen Bereich der Untersuchung waBriger Losungen erheblich erweitern konnen.

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Cryoenzymology in Aqueous Media

Cited by 10 publications

References 57 publications

Cryoradiolysis and Cryospectroscopy for Studies of Heme-Oxygen Intermediates in Cytochromes P450

Cryoradiolysis and Cryospectroscopy for Studies of Heme-Oxygen Intermediates in Cytochromes P450

The Rapid‐Flow‐Quench Method in the Study of Fast Reactions in Biochemistry: Extension to Subzero Conditions

Anomalien des flüssigen Wassers

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