Cryopreservation: an alternative for the long-term storage of old potato varieties

Schäfer-Menuhr, Angelika; Müller, Ellruth; Mix-Wagner, Gunda

doi:10.1007/bf02358469

Cited by 44 publications

(24 citation statements)

References 5 publications

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“…The vigour was not increasingly reduced compared to the regular variation occuring in the control plants, which had not been cryopreserved (Schäfer-Menuhr et al 1997). RFLP analysis and flow cytometry did not show genetic abnormalities within 161 samples of regrown plants (Schäfer-Menuhr et al 1996a, 1996b. Storage in LN for up to 10 years was found to have no adverse effect on the regeneration rates MixWagner et al 2003).…”

Section: Genomic Dna Stabilitymentioning

confidence: 85%

“…Morphological and phenotypic (Barandalla et al 2003;Benson et al 1996;Harding and Benson 1994;Hirai and Sakai 2000;Mix-Wagner 1999;Perazzo et al 1999Perazzo et al -2000Schäfer-Menuhr 1996a, 1996bZarghami et al 2008), cytological (Barandalla et al 2003;Benson et al 1996;Mix-Wagner 1999;Schäfer-Menuhr et al 1996a, 1996bWard et al 1993;Zarghami et al 2008) and molecular (Harding 1991;Harding and Benson 2000;Harding and Benson 2001;Hirai and Sakai 1999;Mix-Wagner 1999;Schäfer-Menuhr et al 1996a, 1996bZarghami et al 2008) comparisons were conducted revealing that plant material was genetically stable as a result of cryopreservation.…”

Section: Genomic Dna Stabilitymentioning

confidence: 99%

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Potato Shoot Tip Cryopreservation. A Review

2010

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Potato is one of the most important crops worldwide. Genetic resources of potato (Solanum tuberosum L. ssp. tuberosum) and related cultivated species are conserved through storage of tubers, in vitro plants and in cryopreservation. Cryopreservation, storage in or above liquid nitrogen, is the best option to maintain vegetatively propagated plants in the long term. The present review gives comprehensive information about various cryopreservation techniques for potato published from 1977 until the present. It discusses factors that affect the process and success of cryopreservation, such as donor culture conditions, preculture, cooling, warming and post-culture treatments. Studies are presented that analyse the histological and ultrastructural changes after different cryopreservation steps and the morphological pathways during regeneration of plants after rewarming. The maintenance of genetic stability in potato after cryopreservation has also been demonstrated by various phenotypic and molecular methods. The first thermal analyses on potato shoot tips are presented using differential scanning calorimetry to analyse the state of water during cooling and warming. Biochemical analyses of different compounds, such as soluble sugars and proteins, have been performed to understand and improve existing cryogenic methods. Potato is an example where successful virus elimination has been obtained via cryopreservation of shoot tips (cryotherapy). There are already cryopreserved collections of potato shoot tips in

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Section: Genomic Dna Stabilitymentioning

confidence: 85%

Section: Genomic Dna Stabilitymentioning

confidence: 99%

Potato Shoot Tip Cryopreservation. A Review

2010

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“…Cryoprotocol for potato has to solve the problem of how to overcome temperature between 0 °C and -130 °C during cooling and warming without ice crystal growth and cell damage. Cold acclimation is not appropriate as pre-cultivation for potato plant (Hirai & Sakai, 1999;Schafer-Menuhr et al, 1996;Kaczmarczyk, 2008). The only method for potato vitrification is a water content decrease in samples, and than the rapid cooling and warming rate.…”

Section: Methods Based On Dehydrationmentioning

confidence: 99%

Comparison of Cryopreservation Methods of Vegetatively Propagated Crops Based on Thermal Analysis

Zámečnı́k¹,

Faltus²,

Bilavčík³

et al. 2012

Current Frontiers in Cryopreservation

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“…28 throughput of samples. The droplet method for cryopreserving Solarium species (Schafer-Menuhr et al 1996) fulfils this requirement. This study investigated whether the success of this protocol as applied to potato could equally apply to asparagus, using the well established tissue culture method for asparagus (Abernethy & Conner 1992) for the recovery phase.…”

Section: Resultsmentioning

confidence: 99%

“…reliable tool. In this study we describe a simple cryopreservation method for asparagus, using in vitro cultured shoot tips, by the "droplet" method originally developed for potato (Schafer-Menuhr et al 1996;Mix-Wagner 2000). This method has the added advantage that it requires neither controlled freezing equipment nor expensive apparatus.…”

Section: Introductionmentioning

confidence: 99%

Survival and recovery of asparagus shoot tips after cryopreservation using the “droplet” method

Mix-Wagner¹,

Conner

Cross³

2000

New Zealand Journal of Crop and Horticultural Science

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In vitro cultured shoot tips of asparagus(Asparagus officinalis L.) were successfully cryopreserved using the "droplet" method. Excised shoot tips, pre-treated with liquid cryoprotective medium, were suspended in small droplets (c. 5 (0,1) of cryoprotectant on aluminium foil and then rapidly frozen by direct immersion in liquid nitrogen. Shoot tips of all eight asparagus genotypes tested were successfully cryopreserved, and within 7 days had resumed growth into well developed shoots (5-8 mm long) without intermediate callus formation. High survival and regrowth was achieved without any cold hardening or pre-growth, and ranged from 58% for clone M120 to 96% for cultivar 'Rutgers Beacon', with a mean frequency of c. 80% across all eight genotypes.

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Cryopreservation: an alternative for the long-term storage of old potato varieties

Cited by 44 publications

References 5 publications

Potato Shoot Tip Cryopreservation. A Review

Potato Shoot Tip Cryopreservation. A Review

Comparison of Cryopreservation Methods of Vegetatively Propagated Crops Based on Thermal Analysis

Survival and recovery of asparagus shoot tips after cryopreservation using the “droplet” method

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