Cryopreservation and fertility of ejaculated and epididymal stallion sperm

Monteiro, Gabriel Augusto; Papa, Federico; Zahn, Fabíola Soares; Dell’Aqua, J.A.; Melo, Cely Marini; Maziero, R. R. D.; Avanzi, Bruno Ribeiro; Alvarenga, Marco Antônio; Guasti, Priscilla Nascimento

doi:10.1016/j.anireprosci.2011.08.002

Cited by 82 publications

(60 citation statements)

References 17 publications

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“…The collection and cryopreservation of epididymal spermatozoa from stallions has been proposed over several years [7,9,10,[20][21][22][23], to allow the conservation of semen from stallions with high genetic value which had died suddenly or become permanently unable to Table 1 Sperm parameters (mean AE standard deviation) after collection (C), first washing (W1), second washing (W2), equilibration at 5 C for 75 minutes (EQ), and freezing/thawing (FT), in samples collected from the epididymis of stallions after colic (IS group, n ¼ 5) or routine castration (HS group, n ¼ 7). reproduce.…”

Section: Discussionmentioning

confidence: 99%

Characteristics of frozen epididymal spermatozoa from stallions that died 12 to 36 hours after colic surgery

et al. 2016

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Section: Discussionmentioning

confidence: 99%

Characteristics of frozen epididymal spermatozoa from stallions that died 12 to 36 hours after colic surgery

et al. 2016

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“…The recovery and cryopreservation of spermatozoa from the epididymides of deceased animals (recovery postmortem) is a viable option in maintaining their germplasm available for future use (Tittarelli et al 2006). Studies have shown the efficacy and potential of epididymal spermatozoa to result in fertilization in vitro and in vivo by using these spermatozoa in artificial insemination, in vitro fertilization of embryos and intracytoplasmic sperm injection with satisfactory results (SantiagoMoreno et al 2006;Martins et al 2007;Costa et al 2011;Monteiro et al 2011;Ringleb et al 2011). The cauda epididymis in the mammals is responsible for the storage of viable spermatozoa until the moment of ejaculation and proportions a favorable environment for the preservation of its fertilization capacity for several weeks (Jones 2004).…”

Section: Introductionmentioning

confidence: 99%

Viability of bull spermatozoa collected from the epididymis stored at 18-20°C

Bertol

Weiss

Soccol

et al. 2013

Braz. arch. biol. technol.

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“…Mobility, morphology, DNA integrity, viability of spermatozoa in ejaculated and epididymal sperm have been studied by many researches [7,[24][25][26]. The methodological approaches used by us for the first time made it possible to carry out a comparative analysis of the ultrastructure of germ cells (obtained by different methods and after cryopreservation) on biological material from the same stallions, which eliminated the influence of the individual characteristics of different individuals on the feature to be compared.…”

Section: Introductionmentioning

confidence: 99%

“…A total of 15 to 25 billion spermatozoa are contained in the epididymis [23,24]. Therefore, extraction of spermatozoa from the epididymis allows obtaining a sufficient number of gametes for artificial insemination of both freshly diluted and chilled and cryopreserved sperm.…”

Section: Introductionmentioning

confidence: 99%

Comparative Study of the Structural Integrity of Spermatozoa in Epididymal, Ejaculated and Cryopreserved Semen of Stallions

Atroshchenko¹,

Kalashnikov²,

Bragina³

et al. 2017

S-h. biol.

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A b s t r a c tCryopreservation of semen is an important way to preserve genetic resources. It is the most actual in horse breeding than in other livestock industries, as currently in many horse breeds, especially unique domestic breeds, the number is approaching a critical level. For many native breeds due to a specific management, year-round outdoors in herds, it is impossible to obtain sperm for cryopreservation by a traditional method using an artificial vagina, and the only cost-effective way to create cryobanks is getting epididymal semen. The technology for cryopreservation of stallion semen includes some critical steps that are characterized by a decrease in sperm quality. These are procedure for semen donation, dilution, temperature shock during freezing and thawing. For the first time a comparative transmission electron microscopy study of the ultrastructural integrity of spermatozoa was done for ejaculated and epididymal sperm in the same stallions thus avoiding the influence of different individuals on the compared parameter. Structural damage caused by cryoconservation of epididymal and ejaculated sperm was studied. It was found that acrosomes were the most susceptible and undergone the greatest impact. Its predominant pathology is the absence of internal contents (acrosome hypoplasia), resulted in enzyme deficiency. The frequency of this pathology was 12.4 % and 14.0 % for fresh epididymal and ejaculated sperm, respectively, and increased almost twofold after freezing and thawing, reaching 26.5 and 27.4 %, respectively. The rate of spermatozoa with the second most common pathology, acrosome degradation (premature release of sperm enzymes that dissolve the oocyte membrane, resulting in the loss of the ability to fertilize), after cryopreservation increased by 5.9 % (p < 0.05) and 8.9 % (p < 0.01) for epididymal and ejaculated semen, respectively. The nucleus of the sperm is one of the most resistant to cryopreservation among the organelles, though in two stallions we observed changes in the shape of the nucleus and vacuolation of chromatin after cryopreservation with a frequency of 1.6 to 6.1 %. Less than 10 % of the sperm had pathology of mitochondria. The axoneme of sperm is sufficiently resistant to cryopreservation. Outer dense fibers and fibrous membrane are almost not damaged during semen collecting as well as under semen freezing and thawing. Higher rates of ultrastructural integrity found for epididymal semen were not statistically significant. Thus, the sperm collecting results in minimal ultrastructural damage, whereas the main pathology is caused by cryopreservation. The ultrastructural integrity of spermatozoa in epididymal semen allow us to recommend this sperm collecting technique to organize cryobanks in case of impossibility of sperm collecting from stallions in traditional ways, or in need for early castration of stallions, in particular in sporting and productive horse breeding.

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Cryopreservation and fertility of ejaculated and epididymal stallion sperm

Cited by 82 publications

References 17 publications

Characteristics of frozen epididymal spermatozoa from stallions that died 12 to 36 hours after colic surgery

Characteristics of frozen epididymal spermatozoa from stallions that died 12 to 36 hours after colic surgery

Viability of bull spermatozoa collected from the epididymis stored at 18-20°C

Comparative Study of the Structural Integrity of Spermatozoa in Epididymal, Ejaculated and Cryopreserved Semen of Stallions

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