Cryopreservation effects on sperm function and fertility in two threatened crane species

Brown, Megan E.; Singh, Ramveer; Pukazhenthi, Budhan S.; Keefer, Carol L.; Songsasen, Nucharin

doi:10.1016/j.cryobiol.2018.01.010

Cited by 17 publications

(14 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In reference to the type of cryoprotectant, our results showed that DMA had a considerably more damaging effect on motility parameters than DMSO. Although, in a general sense, DMSO is a less explored and used cryoprotectant, our findings also agree with those obtained in turkeys, whooping cranes (Grus americana), and white-naped cranes (Antigone vipio), in which better post-thaw sperm motility, sperm viability, and inner perivitelline membrane binding rates were obtained when DMSO was compared with DMA [21,22]. In contrast, Blanco et al [16] reported an increase in sperm viability and fertility when DMA was used as a cryoprotectant in sandhill crane sperm.…”

Section: Discussionsupporting

confidence: 90%

Optimization of Sperm Cryopreservation Protocol for Peregrine Falcon (Falco peregrinus)

Cardoso

Sánchez-Ajofrín

Castaño³

et al. 2020

Animals

View full text Add to dashboard Cite

Simple Summary: The process of semen cryopreservation can have multiple advantages in an ex situ conservation program. However, there is a necessity to adapt the protocol to the specificity of each species. With that in mind, we aimed to optimize the sperm freezing/thawing process and study the effect of different cryoprotectants in the peregrine falcon.Abstract: Sperm cryopreservation is a complex process that needs to be adapted to wild and domestic avian species to ensure proper efficiency. Because of its accessibility, the peregrine falcon may be used as a good model for studying other raptor species. To find the most optimal cryopreservation protocol for peregrine falcon ejaculates, sperm parameters such as motility, viability, DNA fragmentation, acrosome integrity, and mitochondrial activity were analyzed under different conditions by varying the freezing method (slow freezing in straws vs. ultrarapid freezing in pellets), thawing conditions (37 • C for 30 s vs. 5 • C for 1 min), type of cryoprotectant (DMA vs. DMSO), and concentration of DMSO (4% vs. 8%). Results show that slow cryopreservation in straws yielded greater percentages (p < 0.05) of motile spermatozoa (22.5% ± 4.4% vs. 0.0% ± 4.1%), viable spermatozoa with intact acrosomes (84.6% ± 4.3% vs. 77.4% ± 4.3%), and spermatozoa with active mitochondria (41.0% ± 6.7% vs.12.8% ± 6.7%), compared with those obtained by the ultrarapid freezing in pellets. However, no differences were found between different thawing conditions. Moreover, all sperm motility parameters were greater (p < 0.05) when DMSO was used during freezing compared with DMA, although the use of 3% and 8% DMSO produced similar results. In conclusion, these results represent important progress in the study of falcon semen cryopreservation protocol, highlighting the crucial steps of the process and the most suitable conditions. Animals 2020, 10, 691 2 of 11 existent species. A viable presented solution is the creation of a germplasm bank that contains the sperm, oocytes, or even embryos of the largest possible number of species [2,3].Since the storage of eggs or embryos from birds poses a difficult challenge because of their large size and complexity, sperm cells are a good alternative [4]. Sperm is fairly easy and inexpensive to acquire, and its cryopreservation is much more accessible compared with the oocyte/embryo, because of the small volume of water inside of the sperm [5]. Furthermore, existing technologies allow the semen of many animal species to be collected and used [6]. Frozen semen can be a very important part of ex situ conservation efforts, since it allows for the storage of genetic material from multiple species for a long period of time. Stored semen can be used in reintroduction projects or to enlarge the genetic pool of a fragmented population. It also allows for the selection of individuals of genetic interest and the exchange of samples between distant locations [7,8]. In birds, frozen semen could become an increasingly relevant tool, because captive breeding programs a...

show abstract

Section: Discussionsupporting

confidence: 90%

Optimization of Sperm Cryopreservation Protocol for Peregrine Falcon (Falco peregrinus)

Cardoso

Sánchez-Ajofrín

Castaño³

et al. 2020

Animals

View full text Add to dashboard Cite

show abstract

“…The interaction between the IPVM and spermatozoa can be tested in vitro and thus serves as a functional sperm assay [4,6,7,8,23]. The detection of perivitelline-membrane-bound sperm with fluorescence microscopy is another option to verify the penetration ability of spermatozoa and indicate germ-cell-compatibility.…”

Section: Discussionmentioning

confidence: 99%

“…This technique avoids the problem of separating different parts of the perivitelline membrane, as no separation is necessary. Results in various species, including birds [9,23,24,25], chelonians [26], and crocodiles [27], prove this test to be highly useful.…”

Section: Discussionmentioning

confidence: 99%

“…Surprisingly, the number of penetrating spermatozoa was greater in the investigation with frozen–thawed spermatozoa, which indicates better motility. In general, cryopreservation reduces the quality of an ejaculate [35,36], and there are species-specific differences in susceptibility to cryopreservation [20,23]. The difference in quality may be due to different reasons: the roosters used for collection of the fresh samples had also been allowed to perform natural service intermittent to the investigation.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Modification and Clinical Application of the Inner Perivitelline Membrane Test in Different Avian Species

Krohn

Fischer

Schneider

et al. 2019

Veterinary Sciences

View full text Add to dashboard Cite

The aim of this study was to adapt an inner perivitelline membrane (IPVM) test as an interspecies penetration assay for avian spermatozoa. The IPVM of different bird species was evaluated to test the penetrating ability of avian spermatozoa in an intra- and interspecies design. Isolation of the IPVM via acid hydrolysis was tested in pre-incubated chicken eggs and in six other avian species. The separation protocol was modified (time, acid concentration) to facilitate practicability. Separated membranes were evaluated with dark field microscopy for the presence of holes produced by penetrating spermatozoa. In chicken eggs, the influence of different membrane storage conditions was tested. In the penetration assay, the IPVM of chicken eggs was used as a model for fresh and frozen–thawed rooster sperm and for fresh spermatozoa of cockatiels and falcons. Results demonstrated that the time of egg-incubation had a significantly negative influence on the isolation ability of the IPVM (p < 0.0001). IPVM-separation was successful for a maximum of two days after preincubation. In the experiments with eggs from other avian species, results were heterogenous: there was no isolation in geese and cockatiels, 20% in the European kestrel, and 40% in pheasant, quail, and duck. In the penetration assay, holes were found in 100% of the IPVM of chicken eggs after incubation with native and frozen–thawed rooster semen and in 10% with fresh cockatiel semen. Falcon spermatozoa failed to produce visible holes. In conclusion, the IPVM of chicken eggs seems to be unsuitable to establish a functional sperm assay in other species tested but is suitable for quality evaluation of cryopreserved rooster sperm.

show abstract

“…Different disaccharides (sucrose/trehalose) and/or trisaccharides (raffinose) have been successfully incorporated into ram, red deer, and bull sperm cryopreservation extenders (El-Sheshtawy et al., 2015; Fernandez-Santos et al., 2007; Bucak et al., 2010; Tuncer et al., 2010, 2011; Bucak et al., 2013). In avian species, disaccharides (sucrose) showed contrasted effects on the success of sperm cryopreservation (Blanco et al., 2011; Mosca et al., 2016; Miranda et al., 2017; Brown et al., 2018) while nothing is known about the effect of raffinose on sperm cryopreservation.…”

Section: Introductionmentioning

confidence: 99%

Sucrose increases the quality and fertilizing ability of cryopreserved chicken sperms in contrast to raffinose

Thananurak¹,

Chuaychu-noo²,

Thélie³

et al. 2019

Poultry Science

View full text Add to dashboard Cite

Chicken semen conservation is an important tool for programs of genetic diversity management and of endangered breeds’ conservation. However, the method still needs to be improved in order to be applied in a wide variety of environments and breeds. Our objective was to compare the effects of 2 external cryoprotectants saccharides (sucrose and raffinose) on the sperm freezability of a Thai local breed, Pradu Hang Dum, in which semen was frozen with a simple freezing method using nitrogen vapors and dimethyl formamide (DMF). Thirty-six males were selected on their motility vigor score for the experiments. In a first experiment, a large range of sucrose and raffinose doses were tested. Semen quality was evaluated after incubation at 5°C or after cryopreservation in straws in the saline Blumberger Hahnen Sperma Verdünner diluent + DMF (6% v/v) with or without sucrose/raffinose. The best targeted doses of sucrose and raffinose were then kept for experiment 2 that was focused on cryopreserved semen. In this experiment, semen quality was measured on frozen-thawed sperm: different objective motility data evaluated by computer-assisted sperm analysis (CASA), membrane integrity, acrosome integrity, mitochondria function evaluated using flow cytometry, lipid peroxide production assessed by the thiobarbituric acid test. Fertility obtained with frozen-thawed semen supplemented or not with sucrose or raffinose was also evaluated after artificial insemination of laying hens. The presence of sucrose at the osmotically inactive dose 1 mmol significantly increased the vigor motility, membrane integrity, acrosome integrity, and mitochondrial functions of frozen-thawed sperm ( P < 0.05), and showed the highest levels of fertility after sperm cryopreservation (91% vs. control 86%, P < 0.001). Raffinose showed negative effects on in vitro semen quality from 1 to 100 mmol. Fertility was also negatively ( P < 0.001) affected by raffinose (fertility rate 66 to 70%). We thus showed in the present study the high success of a simple chicken sperm cryopreservation method with an external cryoprotectant easily available and cheap, the sucrose, used at an osmotically inactive low concentration.

show abstract

Cryopreservation effects on sperm function and fertility in two threatened crane species

Cited by 17 publications

References 46 publications

Optimization of Sperm Cryopreservation Protocol for Peregrine Falcon (Falco peregrinus)

Optimization of Sperm Cryopreservation Protocol for Peregrine Falcon (Falco peregrinus)

Modification and Clinical Application of the Inner Perivitelline Membrane Test in Different Avian Species

Sucrose increases the quality and fertilizing ability of cryopreserved chicken sperms in contrast to raffinose

Contact Info

Product

Resources

About