Cryopreservation of adult primate testes

Pothana, Lavanya; Venna, Naresh Kumar; Devi, Lalitha; Singh, Anju; Chatterjee, Ipsita; Goel, Sandeep

doi:10.1007/s10344-016-1024-y

Cited by 5 publications

(5 citation statements)

References 42 publications

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“…17 Among wild species (Table 2), promising results with slow-freezing methods have been reached in nonhuman primates, such as adult white-headed marmosets (Callithrix geoffroyi), mandrills (Mandrillus sphinx), and chimpanzees, where tissues derived from these three species were able to express sperm-and spermatid-specific proteins (PRM2 and TNP1, respectively), proving maintenance of spermiogenesis after cryopreservation. 29 Studies have also highlighted differences between species since mandrill and marmoset testicular tissues can be effectively cryopreserved in media containing 10% dimethyl sulfoxide (DMSO) and 80% fetal bovine serum (FBS), whereas chimpanzee requires only 20% DMSO without FBS. The most promising result 9 who demonstrated the birth of a healthy female derived from intracytoplasmic injection of a sperm produced by the autologous graft of cryopreserved testicular tissue.…”

Section: Slow-and Fast-freezing Methodsmentioning

confidence: 99%

“…Various media can be effectively used for this purpose, such as the sterile saline supplemented with 1% penicillin-streptomycin used in the Thai study 16 or phosphate-buffered saline (PBS) used for Indian spotted mouse deer 15 and chimpanzee (Pan troglodytes). 29 As an alternative, Dulbecco's modified Eagle medium (Gibco) was used for transport of ice-cooled testes of domestic species, such as pigs 30 and dogs. 31 Another important factor related to the cryopreservation is the size of testicular tissue fragments used for the procedure.…”

Section: Collecting and Processing Testicular Tissues Before Cryopreservationmentioning

confidence: 99%

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Cryopreservation and Culture of Testicular Tissues: An Essential Tool for Biodiversity Preservation

Silva

Pereira

Comizzoli

2020

Biopreservation and Biobanking

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Systematic cryo-banking of reproductive tissues could enhance reproductive management and ensure sustainability of rare mammalian genotypes. Testicular tissues contain a vast number of germ cells, including at early stages (spermatogonia and spermatocytes), that can potentially develop into viable spermatozoa after grafting or culture in vitro, and the resulting sperm cells then can be used for assisted reproductive techniques. The objective of this review was to describe current advances, limitations, and perspectives related to the use of testicular tissue preservation as a strategy for the conservation of male fertility. Testes can be obtained from mature or prepubertal individuals, immediately postmortem or by orchiectomy, but testicular biopsies could also be an alternative to collect samples from living individuals. Testicular fragments can be then cryopreserved by using slow or ultra-rapid freezing, or even vitrification methods. The composition of cryopreservation media can vary according to species-specific characteristics, especially regarding the cryoprotectant type and concentration. Finally, spermatozoa have been usually obtained after xenografting of testicular fragments into severely immunodeficient mice, while this method still has to be optimized after in vitro culture conditions.

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Section: Slow-and Fast-freezing Methodsmentioning

confidence: 99%

Section: Collecting and Processing Testicular Tissues Before Cryopreservationmentioning

confidence: 99%

Cryopreservation and Culture of Testicular Tissues: An Essential Tool for Biodiversity Preservation

Silva

Pereira

Comizzoli

2020

Biopreservation and Biobanking

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“…In non-human primates, to the best of our knowledge, there are no comparatives studies between testicular cryopreservation methods. Promising results have been previously obtained using slow freezing in diverse non-human primates, generating also healthy offspring from graft-derived spermatozoa ( 36 , 37 ). Our findings agree with these earlier reports based on the greater viability and DNA integrity found in rounded cells after slow freezing in comparison to vitrification.…”

Section: Discussionmentioning

confidence: 99%

DNA integrity and viability of testicular cells from diverse wild species after slow freezing or vitrification

Peris-Frau

Benito-Blanco²,

Martínez‐Nevado³

et al. 2023

Front. Vet. Sci.

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Introduction and objectiveCryopreservation of testicular tissues offers new possibilities to protect endangered species, genetically valuable individuals or even the fertility potential of prepubertal individuals who have died unexpectedly. However, the use of this technique still remains a challenge. In this study, slow freezing and vitrification of testicular tissue was investigated to find out which cryopreservation method could better preserve the viability and DNA integrity of testicular germ cells in diverse wild species.MethodsTestes were obtained post-mortem from 18 artiodactyls (wild boar, roe deer, dwarf goat, mhor gazelle, European mouflon, African forest buffalo, Malayan tapir, dorcas gazelle, Iberian ibex, gnu, red river hog), 5 primates (colobus monkey, capuchin monkey, mandrill), 8 carnivores (gray wolf, Persian leopard, binturong, European mink, American black bear, suricata), and 2 rodents (Patagonian mara). The testicles belonged to adult individuals and were cut into small pieces and cryopreserved by needle immersed vitrification or uncontrolled slow freezing using a passive cooling device. After warming or thawing, testicular tissues were enzymatically digested and two germ cell types were differentiated based on their morphology: rounded cells (spermatogonia, spermatocytes, and early spermatids) and elongated cells (elongated spermatids and spermatozoa). Cell viability was assessed by SYBR-14/propidium iodide while DNA fragmentation by TUNEL assay with fluorescence microscope.Results and discussionOur preliminary results revealed that our uncontrolled slow freezing method better preserved the viability and DNA integrity of elongated cells than vitrification. Such trend was observed in all species, being significant in artiodactyls, carnivores, and primates. Similarly, the viability and DNA integrity of rounded cells was also better maintained in primates by uncontrolled slow freezing, while in carnivores, vitrification by needle immersion showed better results in this type of cells. In artiodactyls and rodents both techniques preserved the viability of rounded cells in a similar manner, although the DNA integrity of these cells was greater after needle immersed vitrification in artiodactyls.ConclusionsIn conclusion, the effectiveness of each cryopreservation method is affected by the phylogenetic diversity between species and cell type.

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“…Slow freezing methods for testicular tissue have shown promising results with tissue showing maintenance of spermiogenesis after cryopreservation for nonhuman primates including white-headed marmoset ( Calllithrix geoffroyi ) , mandrill ( Mandrillus sphinx ) and chimpanzee ( Pan troglodytes ) ( Pothana et al 2016 ). Following cryopreservation, conditions need to be met to enable the thawed tissue to resume spermatogenesis.…”

Section: Gamete and Reproductive Tissue Cryopreservationmentioning

confidence: 99%

“…The cryopreservation of testicular tissue has been achieved for many wild mammalian species (Table 1), although this is more complex than cell cryopreservation due to increased requirements of permeation of cryoprotectant and increased heterogeneity of the tissue (Pothana et al, 2017). Slow-freezing methods for testicular tissue have shown promising results with tissue showing maintenance of spermiogenesis after cryopreservation for nonhuman primates including white-headed marmoset (Calllithrix geoffroyi), mandrill (Mandrillus sphinx) and chimpanzee (Pan troglodytes) (Pothana et al, 2016). Following cryopreservation, conditions need to be met to enable the thawed tissue to resume spermatogenesis.…”

Section: Introductionmentioning

confidence: 99%

Resurrecting biodiversity: advanced assisted reproductive technologies and biobanking

Bolton¹,

Mooney²,

Pettit

et al. 2022

Reproduction and Fertility

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Biodiversity: the variety of all living organisms on Earth is essential for human survival. However, anthropogenic activities are causing the sixth mass extinction, threatening even our own species. For many animals, dwindling numbers are becoming fragmented populations with low genetic diversity, threatening long-term species viability. With extinction rates 1,000-10,000 times greater than is natural, ex situ and in situ conservation programmes need additional support to save species. The indefinite storage of cryopreserved (-196oC) viable cells and tissues (cryobanking), followed by assisted or advanced assisted reproductive technology (ART: utilisation of oocytes and spermatozoa to generate offspring; aART: utilisation of somatic cell genetic material to generate offspring), may be the only hope for species long-term survival. As such, cryobanking should be considered a necessity for all future conservation strategies. Following cryopreservation, ART/aART can be used to reinstate lost genetics back into a population, resurrecting biodiversity. However, for this to be successful, species-specific protocol optimisation and increased knowledge of basic biology for many taxa is required. Current ART/aART is primarily focused on mammalian taxa, however, this needs to be extended to all, including to some of the most endangered: amphibians. Gamete, reproductive tissue and somatic cell cryobanking can fill the gap between losing genetic diversity today, and future technological developments. This review explores species prioritisation for cryobanking and the successes and challenges of cryopreservation and multiple ARTs/aARTs. We discuss the value of cryobanking before more species are lost and the potential of advanced reproductive technologies not only to halt, but reverse biodiversity loss.

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Cryopreservation of adult primate testes

Cited by 5 publications

References 42 publications

Cryopreservation and Culture of Testicular Tissues: An Essential Tool for Biodiversity Preservation

Cryopreservation and Culture of Testicular Tissues: An Essential Tool for Biodiversity Preservation

DNA integrity and viability of testicular cells from diverse wild species after slow freezing or vitrification

Resurrecting biodiversity: advanced assisted reproductive technologies and biobanking

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