Cryopreservation of ayu spermatozoa

Tsutaka, Madoka; Yamamoto, Shinichi; Naka, Kazuhiro; Shimizu, Tadayori; M, Nakamura; Takii, Kenji; Ohta, Hiromi

doi:10.2331/suisan.72.34

Cited by 8 publications

(6 citation statements)

References 27 publications

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“…It is well known that MeOH is less toxic and that its high permeability provides good survivability with spermatozoa in many teleost species (Harvey, 1983;Leung, 1987;Rana & McAndrew, 1989;Lahnsteiner et al, 1997). Several studies have shown FBS to be an excellent cryopreservation diluent (Aoki et al, 1997;Ohta et al, 2001;Tsutaka et al, 2006). In the present study, no conspicuous differences in post-thaw motility were found between ASP and FBS when 10% MeOH was used as a cryoprotectant.…”

Section: Discussioncontrasting

confidence: 39%

“…Several studies have shown FBS to be an excellent cryopreservation diluent (Aoki et al , 1997; Ohta et al , 2001; Tsutaka et al , 2006). In the present study, no conspicuous differences in post‐thaw motility were found between ASP and FBS when 10% MeOH was used as a cryoprotectant.…”

Section: Discussionmentioning

confidence: 99%

“…Optimum cooling rates for spermatozoa vary among species; for example, from −5 to −15° C min −1 in the oily bitterling Tanakia limbata (Temminck & Schlegel) (Ohta et al , 2001), −40° C min −1 in the Colorado pikeminnow Ptychocheilus lucius Girard (Tiersch et al , 2004) and −42·5° C min –1 in the ayu Plecoglossus altivelis altivelis (Temminck & Schlegel) (Tsutaka et al , 2006). Tiersch (2001) recommended a cooling rate of −40° C min –1 for sperm cryopreservation in a broad range of species.…”

Section: Discussionmentioning

confidence: 99%

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Sperm motility and cryopreservation of spermatozoa in freshwater gobies

Yokoi

Ohta

Hosoya

2008

Journal of Fish Biology

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Testicular sperm motility and methods for the cryopreservation of spermatozoa in the freshwater goby Rhinogobius sp. CB (Cross Band type) were examined. Spermatozoa were almost immotile upon dilution with 300 mOsm kg À1 of NaCl, KCl and mannitol solutions but began to swim in solutions with concentrations <200 mOsm kg À1 . The highest percentage and longest duration of motility was obtained in the 0 and 100-200 mOsm kg À1 solutions, respectively. The highest post-thaw motility, c. 50% of motility before cryopreservation, was obtained when spermatozoa were diluted with an extender of 10% methanol and 90% artificial seminal plasma, cooled at À10Á0 AE 1Á1°C min À1 (mean AE S.E.) to À50°C and plunged into liquid nitrogen. Spermatozoa were cryopreserved in a 50 ml acrylic haematocrit tube to store the small amount of milt. As the cryopreservation method described above was applicable to the endangered Rhinogobius sp. BI (Bonin Island type), it is probable that this method can be used for other species of freshwater gobies.

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Section: Discussioncontrasting

confidence: 39%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Sperm motility and cryopreservation of spermatozoa in freshwater gobies

Yokoi

Ohta

Hosoya

2008

Journal of Fish Biology

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“…The low osmotic pressure of FBS might be unsuitable for sea cucumber sperm. In fish sperm cryopreservation, FBS is reported to be an effective extender for the cryoprotectant (Aoki, Okamoto, Tatsumi, & Ishikawa, ; Ohta, Kawamura, Unuma, & Takegoshi, ; Tsutaka et al, ). In the present study, when FBS was added to seawater at a ratio of 20%, a significantly higher post‐thaw motility was obtained than with ASW alone.…”

Section: Discussionmentioning

confidence: 99%

Motility and fertility of cryopreserved spermatozoa of the Japanese sea cucumber Apostichopus japonicus

et al. 2018

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We developed both a cryopreservation method for Japanese sea cucumber spermatozoa and an artificial fertilization method using post-thaw spermatozoa. Twenty per cent dimethyl sulfoxide (DMSO), 16% foetal bovine serum, and 64% artificial seawater were suitable cryodiluent, and the diluent was pre-cooled to 0°C. Semen was diluted with the solution and enclosed in a 250 μl straw, cooled to −50°C at 10.4 ± 0.4°C/ min, and immediately immersed in liquid nitrogen. Although this method showed the highest post-thaw motility in all the conditions we examined, its post-thaw motility was still less than approximately 15%. Artificial fertilization was carried out by adding post-thaw semen with a cryodiluent to the oocytes. The fertilization rate of 200 oocytes/ml seawater increased with the amount of post-thaw semen from 1 to 5 μl but showed a significant decrease at 25 μl. This decrease was considered to be due to DMSO in the cryodiluent, because the fertilization rate of the fresh semen decreased sharply when the DMSO concentration around the oocytes was 1.0% or more. Further improvement in increasing post-thaw motility and lowering the cryoprotectant concentration is necessary for commercial-scale artificial fertilization.

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“…Cryopreservation was conducted as described previously (Tsutaka, Yamamoto, Naka, Shimizu, Nakamura, Takii & Ohta 2006). Milt was diluted 1:49 with a cryopreservation extender comprising 10% methanol and 90% foetal bovine serum (F6178, Sigma‐Aldrich Japan, Tokyo, Japan) at room temperature (∼20 °C), and the mixture was dispensed into 150 μL acrylic capillary tubes (110 mm in length, 2 mm in diameter; Hematlon ‐ l ; Hayashi Science, Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

Hormone injections enhance the tolerance of land-locked ayu spermatozoa to cryopreservation

Yokoi

Kuwada

Yamane

et al. 2009

Aquaculture Research

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We evaluated the e¡ects of maturation-stimulating hormones on the post-thaw motility of land-locked form ayu (Plecoglossus altivelis) spermatozoa. Male ayu were administered three intraperitoneal injections of either salmon pituitary extract (SPE; 0.2 or 0.6 mg g À 1 BWday À 1 ) or of 17, 20b-dihydroxy-4pregnen-3-one (DHP; 2 or 10 mg g À 1 BWday À 1 ), the maturation-inducing steroid (MIS) in ayu. Before cryopreservation, the motility of spermatozoa of the SPE-and DHP-treated groups was signi¢cantly higher than that of the control group. Similarly, the comparative post-thaw motility (presented as a percentage of the motility obtained before cryopreservation) was signi¢cantly higher in the SPE group than in the control; however, there was no signi¢cant di¡erence between the DHP group and the control. The e¡ect of SPE and DHP on pre-and post-cryopreservation motility was not dose dependent. Our results suggest that the hormone(s) present in salmon pituitary are e¡ective in enhancing the tolerance of ayu sperm cells to cryopreservation and that the MIS (DHP) is not involved in this process.

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Cryopreservation of ayu spermatozoa

Cited by 8 publications

References 27 publications

Sperm motility and cryopreservation of spermatozoa in freshwater gobies

Sperm motility and cryopreservation of spermatozoa in freshwater gobies

Motility and fertility of cryopreserved spermatozoa of the Japanese sea cucumber Apostichopus japonicus

Hormone injections enhance the tolerance of land-locked ayu spermatozoa to cryopreservation

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